Restriction map for
pFN21AE2476
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# NO CUT ENZYMES ( 132 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AgeI AhdI Alw44I AlwNI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AviII
AvrII BalI BamHI BanI BbeI
BbrPI BbvCI BciVI BfaI BfrI
BglII BlnI BlpI BpmI Bpu1102I
BsaAI BsaBI BsaHI BsaI BseAI
BsiEI BsiWI BsmBI BsmFI Bsp1407I
BspDI BspEI BspLU11I BsrBI BsrGI
BssHII BssSI Bst1107I BstAPI BstEII
BstPI BstZ17I CelII ClaI CpoI
DraI DrdI EaeI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoO65I
EcoRV EcoT22I FspI HaeII HgaI
Hin1I HindIII HpaI KasI KpnI
KspI MaeI MamI MfeI MluI
MluNI MroI MscI MunI NarI
NcoI NdeI NheI NotI NruI
NsiI PacI PaeR7I PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RsrII SacI SacII SapI
SbfI ScaI SexAI SfiI SfoI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccI 976 GTCGAC GT^[AC][GT]AC
AccII 45 CGCG CG^CG
AflIII 258 ACGTGT A^C[GA][CT]GT
AocI 418 CCTGAGG CC^T[ACGT]AGG
AspI 1261 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 1351 GGACC G^G[AT]CC
BanII 1209 GAGCCC G[GA]GC[CT]^C
BcgI 1279 GCAAGCAACTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 891 TGATCA T^GATCA
BcnI 95 CCGGG CC^[GC]GG
BglI 91 GCCTCCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaWI 1180 ACCGGA [AT]^CCGG[AT]
BsmI 1406 GAATGC GAATGC(1/-1)
BspHI 783 TCATGA T^CATGA
BsrFI 100 GCCGGC [GA]^CCGG[CT]
BstBI 197 TTCGAA TT^CGAA
BstUI 45 CGCG CG^CG
BstXI 497 CCAATGATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 321 AGATCC [GA]^GATC[CT]
Bsu36I 418 CCTGAGG CC^T[ACGT]AGG
Cfr10I 100 GCCGGC [GA]^CCGG[CT]
DraIII 715 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 562 CTCTTC CTCTTC(1/4)
Eco81I 418 CCTGAGG CC^T[ACGT]AGG
EcoRI 787 GAATTC G^AATTC
EcoT14I 1256 CCAAGG C^C[AT][AT]GG
FbaI 891 TGATCA T^GATCA
FseI 99 GGCCGGCC GGCCGG^CC
Ksp632I 562 CTCTTC CTCTTC(1/4)
MflI 321 AGATCC [GA]^GATC[CT]
MspA1I 761 CAGCTG C[AC]G^C[GT]G
MvnI 45 CGCG CG^CG
NaeI 100 GCCGGC GCC^GGC
NciI 95 CCGGG CC^[GC]GG
NgoMIV 100 GCCGGC G^CCGGC
NspI 1231 GCATGT [GA]CATG^[CT]
NspV 197 TTCGAA TT^CGAA
PflFI 1261 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 586 CCAAGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 752 GAGTC GAGTC(4/5)
PpuMI 1350 AGGACCT [GA]G^G[AT]CC[CT]
PstI 107 CTGCAG CTGCA^G
PvuII 761 CAGCTG CAG^CTG
RcaI 783 TCATGA T^CATGA
SalI 976 GTCGAC G^TCGAC
SfuI 197 TTCGAA TT^CGAA
StyI 1256 CCAAGG C^C[AT][AT]GG
Tsp45I 721 GTGAC ^GT[GC]AC
Tth111I 1261 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 586 CCAAGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 321 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AcsI 787 GAATTC [GA]^AATT[CT]
1149 AAATTT
ApoI 787 GAATTC [GA]^AATT[CT]
1149 AAATTT
AspHI 34 GTGCTC G[AT]GC[AT]^C
284 GTGCTC
BseRI 1418 CTCCTC GAGGAG(10/8)
553 GAGGAG
BsgI 49 CTGCAC GTGCAG(16/14)
598 GTGCAG
BsiHKAI 34 GTGCTC G[AT]GC[AT]^C
284 GTGCTC
BsiYI 586 CCAAGCTGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1181 CCGGAAAGTGG
BslI 586 CCAAGCTGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1181 CCGGAAAGTGG
BsmAI 225 GAGAC GTCTC(1/5)
1399 GAGAC
BspMI 600 GCAGGT ACCTGC(4/8)
814 GCAGGT
BsrDI 567 CATTGC GCAATG(2/0)
776 CATTGC
BstNI 218 CCTGG CC^[AT]GG
1365 CCAGG
DraII 557 AGGCCCT [GA]G^G[ACGT]CC[CT]
1350 AGGACCT
EcoO109I 557 AGGCCCT [GA]G^G[ACGT]CC[CT]
1350 AGGACCT
EcoRII 218 CCTGG ^CC[AT]GG
1365 CCAGG
MaeII 203 ACGT A^CGT
258 ACGT
MaeIII 721 GTGAC ^GT[ACGT]AC
1076 GTTAC
MvaI 218 CCTGG CC^[AT]GG
1365 CCAGG
NlaIV 40 GGGGCC GG[ACGT]^[ACGT]CC
97 GGGGCC
#########################################
# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaI
BbsI BbvI BmyI BpuAI BsaJI
BsoBI Bsp1286I BsrI BssKI BstF5I
Cac8I CfoI Cfr13I DdeI DpnII
DsaI FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HincII HindII
HinfI HpaII HphI ItaI MboI
MboII MnlI MseI MslI MspI
MwoI NdeII NlaIII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI TaqI
TfiI Tru9I TseI Tsp509I TspRI
TthHB8I