##################################
# NO CUT ENZYMES (  194 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcyI        AflII       AflIII      AgeI        AhdI        
AlwI        AocI        Aor51HI     ApaI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspI        AvaI        AvaII       AviII       AvrII       
BalI        BamHI       BanI        BanII       BbeI        
BbrPI       BbvCI       BcgI        BciVI       BclI        
BcnI        BfrI        BglI        BlnI        BlpI        
Bpu1102I    BsaAI       BsaBI       BsaHI       BsaI        
BsaJI       BsaWI       BseAI       BseRI       BsiEI       
BsiWI       BsiYI       BslI        BsmAI       BsmBI       
BsmFI       BsoBI       Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BspMI       BsrBI       BsrFI       
BsrGI       BssHII      BssKI       BssSI       Bst1107I    
BstBI       BstEII      BstF5I      BstNI       BstPI       
BstUI       BstXI       BstZ17I     Bsu36I      CelII       
CfoI        Cfr10I      Cfr13I      ClaI        CpoI        
DraI        DraII       DraIII      DrdI        DsaI        
EaeI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO109I    EcoO65I     
EcoRI       EcoRII      EcoRV       EcoT14I     EcoT22I     
FbaI        FokI        FseI        FspI        HaeII       
HaeIII      HapII       HgaI        HhaI        Hin1I       
HinP1I      HpaI        HpaII       HphI        KasI        
KpnI        KspI        MaeII       MamI        MluI        
MluNI       MroI        MscI        MslI        MspI        
MvaI        MvnI        NaeI        NarI        NciI        
NcoI        NgoMIV      NheI        NlaIII      NlaIV       
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PleI        PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PvuI        RcaI        
RsrII       SacI        SacII       SalI        SapI        
Sau96I      SbfI        ScaI        ScrFI       SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        StyI        SwaI        
TaqI        Tth111I     TthHB8I     Van91I      XcmI        
XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   36 enzymes) #
###################################

AccI           79  GTAGAC               GT^[AC][GT]AC
AciI          616  CCGC                 CCGC(-3/-1)
AcsI          327  AAATTT               [GA]^AATT[CT]
AfaI           69  GTAC                 GT^AC
Alw44I        357  GTGCAC               G^TGCAC
ApaLI         357  GTGCAC               G^TGCAC
ApoI          327  AAATTT               [GA]^AATT[CT]
AspHI         357  GTGCAC               G[AT]GC[AT]^C
BbsI          751  GAAGAC               GAAGAC(2/6)
BfaI          586  CTAG                 C^TAG
BglII         405  AGATCT               A^GATCT
BmyI          357  GTGCAC               G[AGT]GC[ACT]^C
BpuAI         751  GAAGAC               GAAGAC(2/6)
BsgI            5  GTGCAG               GTGCAG(16/14)
BsiHKAI       357  GTGCAC               G[AT]GC[AT]^C
Bsp1286I      357  GTGCAC               G[AGT]GC[ACT]^C
BsrDI         157  GCAATG               GCAATG(2/0)
BstAPI          7  GCAGCTTCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI         405  AGATCT               [GA]^GATC[CT]
Cac8I          96  GCGTGC               GC[ACGT]^[ACGT]GC
FauI          615  CCCGC                CCCGC(4/6)
HindIII       724  AAGCTT               A^AGCTT
MaeI          586  CTAG                 C^TAG
MaeIII        317  GTGAC                ^GT[ACGT]AC
MflI          405  AGATCT               [GA]^GATC[CT]
MspA1I        394  CAGCTG               C[AC]G^C[GT]G
MwoI            7  GCAGCTTCTGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeI           40  CATATG               CA^TATG
PstI          742  CTGCAG               CTGCA^G
PvuII         394  CAGCTG               CAG^CTG
RsaI           69  GTAC                 GT^AC
SfaNI         423  GCATC                GCATC(5/9)
SfcI          742  CTGCAG               C^T[GA][CT]AG
Tsp45I        317  GTGAC                ^GT[GC]AC
XbaI          585  TCTAGA               T^CTAGA
XhoII         405  AGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   11 enzymes) #
###################################

BsmI          652  GAATGC               GAATGC(1/-1)
              438  GCATTC              
BsrI          569  ACTGG                ACTGG(1/-1)
               76  CCAGT               
EarI          289  GAAGAG               CTCTTC(1/4)
              481  GAAGAG              
HincII        162  GTTGAC               GT[CT]^[GA]AC
              487  GTTGAC              
HindII        162  GTTGAC               GT[CT]^[GA]AC
              487  GTTGAC              
HinfI         262  GAATC                G^A[ACGT]TC
              582  GATTC               
Ksp632I       289  GAAGAG               CTCTTC(1/4)
              481  GAAGAG              
MfeI           91  CAATTG               C^AATTG
              505  CAATTG              
MunI           91  CAATTG               C^AATTG
              505  CAATTG              
TfiI          262  GAATC                G^A[AT]TC
              582  GATTC               
TspRI         355  CAGTG                CA[GC]TG[ACGT][ACGT]^
              687  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (   17 enzymes) #
#########################################

AluI        AlwNI       BbvI        BpmI        DdeI        
DpnII       Fnu4HI      ItaI        MboI        MboII       
MnlI        MseI        NdeII       Sau3AI      Tru9I       
TseI        Tsp509I