Restriction map for
pFN21AE3310
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# NO CUT ENZYMES ( 110 enzymes) #
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Acc65I AclI AflIII AgeI Alw44I
Aor51HI ApaI ApaLI AscI Asp718I
AviII AvrII BalI BbeI BbrPI
BcgI BclI BglI BlnI BlpI
Bpu1102I BsaAI BsiEI BsiWI BsmBI
BsmI Bsp1407I BspDI BspHI BspLU11I
BspMI BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstXI BstZ17I CelII Cfr10I ClaI
CpoI DraI DsaI EaeI EagI
EclXI Eco47III Eco52I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HaeII HindIII HpaI KasI KpnI
KspI MfeI MluI MluNI MscI
MunI NaeI NarI NcoI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflMI PinAI PmaCI
PmeI PmlI PshAI Psp1406I PstI
PvuI PvuII RcaI RsrII SacII
SbfI ScaI SfiI SfoI SfuI
SgrAI SmaI SnaBI Sse8387I SspBI
SwaI Van91I XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 67 enzymes) #
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AatII 1672 GACGTC GACGT^C
AccI 2357 GTCGAC GT^[AC][GT]AC
AccII 1639 CGCG CG^CG
AccIII 112 TCCGGA T^CCGGA
AcsI 1459 GAATTT [GA]^AATT[CT]
AcyI 1672 GACGTC G[GA]^CG[CT]C
AfaI 55 GTAC GT^AC
AflII 1792 CTTAAG C^TTAAG
AocI 655 CCTCAGG CC^T[ACGT]AGG
ApoI 1459 GAATTT [GA]^AATT[CT]
AseI 2040 ATTAAT AT^TAAT
AsnI 2040 ATTAAT AT^TAAT
Asp700I 796 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 487 GAGCTC G[AT]GC[AT]^C
AspI 1669 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 890 CCCGAG C^[CT]CG[GA]G
BamHI 837 GGATCC G^GATCC
BbvCI 1711 GCTGAGG CCTCAGC(-5/-2)
BciVI 1020 GGATAC GTATCC(6/5)
BcnI 1899 CCGGG CC^[GC]GG
BfrI 1792 CTTAAG C^TTAAG
BsaBI 334 GATGTCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1672 GACGTC G[GA]^CG[CT]C
BsaWI 112 TCCGGA [AT]^CCGG[AT]
BseAI 112 TCCGGA T^CCGGA
BsiHKAI 487 GAGCTC G[AT]GC[AT]^C
BsmFI 2260 GTCCC GGGAC(10/14)
BsoBI 890 CCCGAG C^[CT]CG[GA]G
BspEI 112 TCCGGA T^CCGGA
BsrDI 1397 GCAATG GCAATG(2/0)
BstAPI 1923 GCAAGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 1639 CGCG CG^CG
Bsu36I 655 CCTCAGG CC^T[ACGT]AGG
CfoI 1626 GCGC GCG^C
DraIII 2416 CACGCAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2281 GACTATGTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 655 CCTCAGG CC^T[ACGT]AGG
EcoNI 2187 CCTGGAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 463 CCCGC CCCGC(4/6)
HgaI 958 GCGTC GACGC(5/10)
HhaI 1626 GCGC GCG^C
Hin1I 1672 GACGTC G[GA]^CG[CT]C
HinP1I 1626 GCGC G^CGC
HincII 2357 GTCGAC GT[CT]^[GA]AC
HindII 2357 GTCGAC GT[CT]^[GA]AC
MamI 334 GATGTCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 112 TCCGGA T^CCGGA
MslI 424 CATGCAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 18 CAGCGG C[AC]G^C[GT]G
MvnI 1639 CGCG CG^CG
NciI 1899 CCGGG CC^[GC]GG
NspI 423 GCATGC [GA]CATG^[CT]
PflFI 1669 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 2040 ATTAAT AT^TAAT
RsaI 55 GTAC GT^AC
SacI 487 GAGCTC GAGCT^C
SalI 2357 GTCGAC G^TCGAC
SapI 796 GAAGAGC GCTCTTC(1/4)
SexAI 384 ACCTGGT A^CC[AT]GGT
SfcI 1778 CTGTAG C^T[GA][CT]AG
SpeI 157 ACTAGT A^CTAGT
SphI 423 GCATGC GCATG^C
SspI 11 AATATT AAT^ATT
StuI 2059 AGGCCT AGG^CCT
Tth111I 1669 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 2124 TCTAGA T^CTAGA
XmnI 796 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 12 enzymes) #
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AhdI 1384 GACAGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1570 GACTTTCTGTC
AspEI 1384 GACAGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1570 GACTTTCTGTC
BanII 217 GAGCCC G[GA]GC[CT]^C
487 GAGCTC
BbsI 1299 GAAGAC GAAGAC(2/6)
1717 GAAGAC
BpmI 490 CTCCAG CTGGAG(16/14)
2228 CTGGAG
BpuAI 1299 GAAGAC GAAGAC(2/6)
1717 GAAGAC
Eam1105I 1384 GACAGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1570 GACTTTCTGTC
HapII 113 CCGG C^CGG
1899 CCGG
HpaII 113 CCGG C^CGG
1899 CCGG
MspI 113 CCGG C^CGG
1899 CCGG
NdeI 1060 CATATG CA^TATG
1564 CATATG
SmlI 1792 CTTAAG C^T[CT][GA]AG
1891 CTCAAG
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# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AciI AluI AlwI AlwNI AvaII
BanI BbvI BfaI BglII BmyI
BsaI BsaJI BseRI BsgI BsiYI
BslI BsmAI Bsp1286I BsrI BssKI
BstF5I BstNI BstYI Cac8I Cfr13I
DdeI DpnII DraII EarI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HinfI HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MvaI MwoI NdeII
NlaIII NlaIV PleI PpuMI Sau3AI
Sau96I ScrFI SfaNI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII