Restriction map for
pFN21AE4144
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# NO CUT ENZYMES ( 108 enzymes) #
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AatII Acc65I AccI AclI AflII
AflIII AgeI Alw44I ApaLI AscI
AseI AsnI Asp700I Asp718I AvaI
AviII BamHI BbeI BbvCI BcgI
BfrI BglI BglII BlpI Bpu1102I
BsaBI BsaI BsiEI BsiWI BsmI
BsoBI Bsp1407I BspDI BspLU11I BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstXI BstZ17I CelII Cfr10I
ClaI CpoI DraIII DrdI EagI
EclXI Eco52I EcoNI EcoRV FseI
FspI HincII HindII HindIII HpaI
KasI KpnI KspI MamI MfeI
MluI MunI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PinAI PmeI
PshBI Psp1406I PstI PvuI PvuII
RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
Sse8387I SspBI SwaI XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 68 enzymes) #
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AccII 730 CGCG CG^CG
AccIII 918 TCCGGA T^CCGGA
AcyI 1057 GGCGTC G[GA]^CG[CT]C
AhdI 1655 GACAGCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 314 CAGAATCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1603 CCTCAGG CC^T[ACGT]AGG
ApaI 912 GGGCCC GGGCC^C
AspEI 1655 GACAGCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1310 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 781 CCTAGG C^CTAGG
BalI 1247 TGGCCA TGG^CCA
BanI 1080 GGTGCC G^G[CT][GA]CC
BbrPI 1159 CACGTG CAC^GTG
BbsI 1561 GAAGAC GAAGAC(2/6)
BciVI 154 GGATAC GTATCC(6/5)
BclI 824 TGATCA T^GATCA
BcnI 72 CCGGG CC^[GC]GG
BlnI 781 CCTAGG C^CTAGG
BpuAI 1561 GAAGAC GAAGAC(2/6)
BsaAI 1159 CACGTG [CT]AC^GT[GA]
BsaHI 1057 GGCGTC G[GA]^CG[CT]C
BseAI 918 TCCGGA T^CCGGA
BsmAI 980 GAGAC GTCTC(1/5)
BsmBI 980 GAGACG CGTCTC(1/5)
BspEI 918 TCCGGA T^CCGGA
BspHI 590 TCATGA T^CATGA
BsrBI 568 GAGCGG CCGCTC(-3/-3)
BsrDI 608 CATTGC GCAATG(2/0)
BstEII 193 GGTAACC G^GT[ACGT]ACC
BstPI 193 GGTAACC G^GT[ACGT]ACC
BstUI 730 CGCG CG^CG
BstYI 833 AGATCC [GA]^GATC[CT]
Bsu36I 1603 CCTCAGG CC^T[ACGT]AGG
DraI 655 TTTAAA TTT^AAA
Eam1105I 1655 GACAGCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1603 CCTCAGG CC^T[ACGT]AGG
EcoO65I 193 GGTAACC G^GT[ACGT]ACC
EcoRI 241 GAATTC G^AATTC
EcoT22I 650 ATGCAT ATGCA^T
FbaI 824 TGATCA T^GATCA
HgaI 1058 GCGTC GACGC(5/10)
Hin1I 1057 GGCGTC G[GA]^CG[CT]C
MflI 833 AGATCC [GA]^GATC[CT]
MluNI 1247 TGGCCA TGG^CCA
MroI 918 TCCGGA T^CCGGA
MscI 1247 TGGCCA TGG^CCA
MseI 656 TTAA T^TAA
MslI 1422 CACCCCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 438 CCGCTG C[AC]G^C[GT]G
MvnI 730 CGCG CG^CG
NciI 72 CCGGG CC^[GC]GG
NsiI 650 ATGCAT ATGCA^T
NspI 933 GCATGC [GA]CATG^[CT]
PflFI 1310 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1159 CACGTG CAC^GTG
PmlI 1159 CACGTG CAC^GTG
PpuMI 1502 AGGTCCC [GA]G^G[AT]CC[CT]
PshAI 436 GACCGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 590 TCATGA T^CATGA
SacI 1368 GAGCTC GAGCT^C
SfcI 1545 CTACAG C^T[GA][CT]AG
SphI 933 GCATGC GCATG^C
SspI 1646 AATATT AAT^ATT
Tru9I 656 TTAA T^TAA
Tsp45I 1583 GTCAC ^GT[GC]AC
TspRI 642 CACTG CA[GC]TG[ACGT][ACGT]^
Tth111I 1310 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 833 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 20 enzymes) #
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AcsI 241 GAATTC [GA]^AATT[CT]
329 AAATTC
Aor51HI 25 AGCGCT AGC^GCT
1594 AGCGCT
ApoI 241 GAATTC [GA]^AATT[CT]
329 AAATTC
AspHI 1292 GAGCAC G[AT]GC[AT]^C
1368 GAGCTC
BsgI 67 CTGCAC GTGCAG(16/14)
13 GTGCAG
BsiHKAI 1292 GAGCAC G[AT]GC[AT]^C
1368 GAGCTC
DdeI 144 CTCAG C^T[ACGT]AG
1604 CTCAG
DsaI 614 CCACGG C^C[GA][CT]GG
1026 CCGTGG
EaeI 1023 TGGCCG [CT]^GGCC[GA]
1247 TGGCCA
EarI 632 CTCTTC CTCTTC(1/4)
1013 GAAGAG
Eco47III 25 AGCGCT AGC^GCT
1594 AGCGCT
FauI 508 CCCGC CCCGC(4/6)
1277 GCGGG
HaeII 25 AGCGCT [GA]GCGC^[CT]
1594 AGCGCT
Ksp632I 632 CTCTTC CTCTTC(1/4)
1013 GAAGAG
MaeII 1160 ACGT A^CGT
1681 ACGT
MaeIII 194 GTAAC ^GT[ACGT]AC
1583 GTCAC
PflMI 871 CCAGATGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
903 CCAGGTCATGG
PleI 1314 GAGTC GAGTC(4/5)
1581 GAGTC
StuI 993 AGGCCT AGG^CCT
1553 AGGCCT
Van91I 871 CCAGATGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
903 CCAGGTCATGG
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# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BanII BbvI BfaI BmyI BpmI
BsaJI BsaWI BseRI BsiYI BslI
BsmFI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI Cac8I CfoI Cfr13I
DpnII DraII EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
MaeI MboI MboII MnlI MspI
MvaI MwoI NdeII NlaIII NlaIV
RsaI Sau3AI Sau96I ScrFI SfaNI
StyI TaqI TfiI TseI Tsp509I
TthHB8I XcmI