Restriction map for
pFN21AE9397
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# NO CUT ENZYMES ( 21 enzymes) #
##################################
AatII AclI AscI BsaBI BspDI
BssHII Bst1107I BstZ17I ClaI MamI
MluI NotI NruI PacI PmeI
Psp1406I PvuI SalI SpeI SspI
SwaI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AccIII 10739 TCCGGA T^CCGGA
AflII 12636 CTTAAG C^TTAAG
AgeI 3570 ACCGGT A^CCGGT
AhdI 2902 GACCCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AseI 6073 ATTAAT AT^TAAT
AsnI 6073 ATTAAT AT^TAAT
Asp700I 8752 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 2902 GACCCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BfrI 12636 CTTAAG C^TTAAG
BglII 3620 AGATCT A^GATCT
BseAI 10739 TCCGGA T^CCGGA
BsiWI 5749 CGTACG C^GTACG
BspEI 10739 TCCGGA T^CCGGA
BstEII 4509 GGTGACC G^GT[ACGT]ACC
BstPI 4509 GGTGACC G^GT[ACGT]ACC
CpoI 2209 CGGTCCG CG^G[AT]CCG
DraI 11928 TTTAAA TTT^AAA
DrdI 4108 GACAAATTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 2902 GACCCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 4509 GGTGACC G^GT[ACGT]ACC
EcoRI 6670 GAATTC G^AATTC
FseI 5380 GGCCGGCC GGCCGG^CC
HpaI 3613 GTTAAC GTT^AAC
KspI 7493 CCGCGG CCGC^GG
MfeI 10180 CAATTG C^AATTG
MroI 10739 TCCGGA T^CCGGA
MunI 10180 CAATTG C^AATTG
PinAI 3570 ACCGGT A^CCGGT
PshAI 15079 GACATGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 6073 ATTAAT AT^TAAT
RsrII 2209 CGGTCCG CG^G[AT]CCG
SacII 7493 CCGCGG CCGC^GG
SbfI 833 CCTGCAGG CCTGCA^GG
ScaI 4745 AGTACT AGT^ACT
SnaBI 4537 TACGTA TAC^GTA
Sse8387I 833 CCTGCAGG CCTGCA^GG
XbaI 14016 TCTAGA T^CTAGA
XmnI 8752 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 25 enzymes) #
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AviII 6615 TGCGCA TGC^GCA
16169 TGCGCA
BbrPI 1198 CACGTG CAC^GTG
3563 CACGTG
BclI 10499 TGATCA T^GATCA
15992 TGATCA
Bsp1407I 8646 TGTACA T^GTACA
10596 TGTACA
BsrGI 8646 TGTACA T^GTACA
10596 TGTACA
BssSI 3211 CACGAG CACGAG(-5/-1)
3941 CTCGTG
BstBI 4756 TTCGAA TT^CGAA
11114 TTCGAA
EagI 1459 CGGCCG C^GGCCG
15709 CGGCCG
EclXI 1459 CGGCCG C^GGCCG
15709 CGGCCG
Eco52I 1459 CGGCCG C^GGCCG
15709 CGGCCG
EcoRV 9187 GATATC GAT^ATC
10252 GATATC
EcoT22I 4585 ATGCAT ATGCA^T
15400 ATGCAT
FbaI 10499 TGATCA T^GATCA
15992 TGATCA
FspI 6615 TGCGCA TGC^GCA
16169 TGCGCA
NdeI 7127 CATATG CA^TATG
10060 CATATG
NheI 10368 GCTAGC G^CTAGC
15493 GCTAGC
NsiI 4585 ATGCAT ATGCA^T
15400 ATGCAT
NspV 4756 TTCGAA TT^CGAA
11114 TTCGAA
PaeR7I 5506 CTCGAG C^TCGAG
9640 CTCGAG
PmaCI 1198 CACGTG CAC^GTG
3563 CACGTG
PmlI 1198 CACGTG CAC^GTG
3563 CACGTG
SfuI 4756 TTCGAA TT^CGAA
11114 TTCGAA
SgrAI 3569 CACCGGTG C[GA]^CCGG[CT]G
6431 CGCCGGCG
SspBI 8646 TGTACA T^GTACA
10596 TGTACA
XhoI 5506 CTCGAG C^TCGAG
9640 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 174 enzymes) #
#########################################
Acc65I AccI AccII AciI AcsI
AcyI AfaI AflIII AluI Alw44I
AlwI AlwNI AocI Aor51HI ApaI
ApaLI ApoI Asp718I AspHI AspI
AvaI AvaII AvrII BalI BamHI
BanI BanII BbeI BbsI BbvCI
BbvI BcgI BciVI BcnI BfaI
BglI BlnI BlpI BmyI BpmI
Bpu1102I BpuAI BsaAI BsaHI BsaI
BsaJI BsaWI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsmI BsoBI Bsp1286I BspHI
BspLU11I BspMI BsrBI BsrDI BsrFI
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstXI BstYI Bsu36I Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
EarI Eco47III Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HinfI HpaII HphI ItaI KasI
KpnI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NarI
NciI NcoI NdeII NgoMIV NlaIII
NlaIV NspI PflFI PflMI PleI
PpuMI PstI PvuII RcaI RsaI
SacI SapI Sau3AI Sau96I ScrFI
SexAI SfaNI SfcI SfiI SfoI
SmaI SmlI SphI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Tth111I TthHB8I Van91I
XcmI XhoII XmaCI XmaI