Restriction map for
pFN21AE3150
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# NO CUT ENZYMES ( 154 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcsI AcyI AflII AflIII AhdI
Alw44I AlwNI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp718I
AspEI AspHI AspI AvaI AviII
AvrII BalI BamHI BanI BanII
BbeI BbrPI BbvCI BciVI BclI
BcnI BfaI BfrI BglI BlnI
BlpI BmyI Bpu1102I BsaAI BsaHI
BsaI BseAI BsiHKAI BsiWI BsmBI
BsoBI Bsp1286I Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstUI BstXI BstZ17I Cac8I
CelII CfoI ClaI CpoI DraI
DraII DraIII DrdI DsaI Eam1105I
Eco47III EcoNI EcoO109I EcoRI FauI
FbaI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I KasI KpnI
KspI MaeI MfeI MluI MluNI
MroI MscI MunI MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NlaIV NotI NruI NspV
PacI PaeR7I PflFI PflMI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
StuI SwaI Tth111I Van91I XbaI
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccI 848 GTAGAC GT^[AC][GT]AC
AgeI 722 ACCGGT A^CCGGT
AocI 752 CCTTAGG CC^T[ACGT]AGG
AvaII 207 GGACC G^G[AT]CC
BbsI 790 GAAGAC GAAGAC(2/6)
BcgI 951 GCATTTCTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpmI 21 CTGGAG CTGGAG(16/14)
BpuAI 790 GAAGAC GAAGAC(2/6)
BsaBI 71 GATGTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaJI 305 CCTTGG C^C[ACGT][ACGT]GG
BsaWI 722 ACCGGT [AT]^CCGG[AT]
BseRI 554 GAGGAG GAGGAG(10/8)
BsiEI 1010 CGGCCG CG[GA][CT]^CG
BsmFI 740 GGGAC GGGAC(10/14)
BsrFI 722 ACCGGT [GA]^CCGG[CT]
BstEII 762 GGTGACC G^GT[ACGT]ACC
BstPI 762 GGTGACC G^GT[ACGT]ACC
Bsu36I 752 CCTTAGG CC^T[ACGT]AGG
Cfr10I 722 ACCGGT [GA]^CCGG[CT]
Cfr13I 207 GGACC G^G[ACGT]CC
EaeI 1010 CGGCCG [CT]^GGCC[GA]
EagI 1010 CGGCCG C^GGCCG
EclXI 1010 CGGCCG C^GGCCG
Eco52I 1010 CGGCCG C^GGCCG
Eco81I 752 CCTTAGG CC^T[ACGT]AGG
EcoO65I 762 GGTGACC G^GT[ACGT]ACC
EcoRV 352 GATATC GAT^ATC
EcoT14I 305 CCTTGG C^C[AT][AT]GG
EcoT22I 185 ATGCAT ATGCA^T
HapII 723 CCGG C^CGG
HincII 408 GTTAAC GT[CT]^[GA]AC
HindII 408 GTTAAC GT[CT]^[GA]AC
HindIII 811 AAGCTT A^AGCTT
HpaI 408 GTTAAC GTT^AAC
HpaII 723 CCGG C^CGG
MaeII 772 ACGT A^CGT
MamI 71 GATGTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspI 723 CCGG C^CGG
MwoI 627 GCAACAACAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NsiI 185 ATGCAT ATGCA^T
NspI 701 ACATGC [GA]CATG^[CT]
PinAI 722 ACCGGT A^CCGGT
PleI 988 GACTC GAGTC(4/5)
PstI 966 CTGCAG CTGCA^G
SapI 172 GAAGAGC GCTCTTC(1/4)
Sau96I 207 GGACC G^G[ACGT]CC
SfaNI 187 GCATC GCATC(5/9)
SmlI 336 CTCAAG C^T[CT][GA]AG
StyI 305 CCTTGG C^C[AT][AT]GG
TfiI 76 GAATC G^A[AT]TC
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# TWO CUT ENZYMES ( 16 enzymes) #
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AciI 1045 CCGC CCGC(-3/-1)
1009 GCGG
Asp700I 438 GAATTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
610 GAAACTATTC
BglII 25 AGATCT A^GATCT
510 AGATCT
BsgI 697 CTGCAC GTGCAG(16/14)
1002 CTGCAC
BsmAI 941 GAGAC GTCTC(1/5)
1041 GTCTC
BsmI 542 GCATTC GAATGC(1/-1)
971 GCATTC
BsrI 20 ACTGG ACTGG(1/-1)
405 CCAGT
HinfI 76 GAATC G^A[ACGT]TC
988 GACTC
MslI 178 CATGAATATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1032 CACGCCTGTG
MspA1I 799 CAGCTG C[AC]G^C[GT]G
1023 CAGCTG
PvuII 799 CAGCTG CAG^CTG
1023 CAGCTG
SfcI 637 CTACAG C^T[GA][CT]AG
966 CTGCAG
SspI 159 AATATT AAT^ATT
589 AATATT
TaqI 190 TCGA T^CGA
1058 TCGA
TthHB8I 190 TCGA T^CGA
1058 TCGA
XmnI 438 GAATTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
610 GAAACTATTC
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# 3 OR MORE CUT ENZYMES ( 38 enzymes) #
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AfaI AluI AlwI BbvI BsiYI
BslI BssKI BstF5I BstNI BstYI
DdeI DpnII EarI EcoRII Fnu4HI
FokI HaeIII HphI ItaI Ksp632I
MaeIII MboI MboII MflI MnlI
MseI MvaI NdeII NlaIII RsaI
Sau3AI ScrFI Tru9I TseI Tsp45I
Tsp509I TspRI XhoII