Restriction map for
pFN21AE4028
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# NO CUT ENZYMES ( 159 enzymes) #
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AatII Acc65I AccI AccII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AviII
AvrII BanI BbeI BbrPI BbsI
BbvCI BcgI BclI BcnI BfrI
BglI BglII BlnI BlpI BpmI
Bpu1102I BpuAI BsaHI BsaI BseRI
BsgI BsiEI BsiHKAI BsiWI BsmBI
BsmI Bsp1407I BspDI BspLU11I BspMI
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstF5I
BstUI BstXI BstZ17I Bsu36I CelII
CfoI Cfr10I ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoRV
EcoT22I FbaI FokI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HincII HindII HindIII HpaI KasI
KpnI KspI MfeI MluI MseI
MunI MvnI NaeI NarI NciI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI RsrII SacI SacII
SalI SapI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SphI Sse8387I SspBI SspI StuI
SwaI Tru9I Tth111I Van91I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AccIII 683 TCCGGA T^CCGGA
AcsI 514 GAATTC [GA]^AATT[CT]
ApoI 514 GAATTC [GA]^AATT[CT]
AvaII 309 GGACC G^G[AT]CC
BalI 623 TGGCCA TGG^CCA
BamHI 233 GGATCC G^GATCC
BanII 100 GGGCTC G[GA]GC[CT]^C
BciVI 273 GGATAC GTATCC(6/5)
BfaI 121 CTAG C^TAG
BmyI 100 GGGCTC G[AGT]GC[ACT]^C
BsaAI 596 TACGTA [CT]AC^GT[GA]
BsaWI 683 TCCGGA [AT]^CCGG[AT]
BseAI 683 TCCGGA T^CCGGA
BsmAI 535 GAGAC GTCTC(1/5)
Bsp1286I 100 GGGCTC G[AGT]GC[ACT]^C
BspEI 683 TCCGGA T^CCGGA
BspHI 198 TCATGA T^CATGA
BssKI 30 CCAGG ^CC[ACGT]GG
BstEII 480 GGTCACC G^GT[ACGT]ACC
BstNI 30 CCAGG CC^[AT]GG
BstPI 480 GGTCACC G^GT[ACGT]ACC
BstYI 233 GGATCC [GA]^GATC[CT]
Cac8I 252 GCCTGC GC[ACGT]^[ACGT]GC
DraII 36 AGGCCCC [GA]G^G[ACGT]CC[CT]
DsaI 626 CCATGG C^C[GA][CT]GG
EaeI 623 TGGCCA [CT]^GGCC[GA]
EcoO109I 36 AGGCCCC [GA]G^G[ACGT]CC[CT]
EcoO65I 480 GGTCACC G^GT[ACGT]ACC
EcoRI 514 GAATTC G^AATTC
EcoRII 30 CCAGG ^CC[AT]GG
HapII 684 CCGG C^CGG
HpaII 684 CCGG C^CGG
MaeI 121 CTAG C^TAG
MflI 233 GGATCC [GA]^GATC[CT]
MluNI 623 TGGCCA TGG^CCA
MroI 683 TCCGGA T^CCGGA
MscI 623 TGGCCA TGG^CCA
MslI 159 CATGACCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 684 CCGG C^CGG
MvaI 30 CCAGG CC^[AT]GG
NcoI 626 CCATGG C^CATGG
PleI 410 GAGTC GAGTC(4/5)
RcaI 198 TCATGA T^CATGA
ScaI 618 AGTACT AGT^ACT
ScrFI 30 CCAGG CC^[ACGT]GG
SfcI 84 CTGTAG C^T[GA][CT]AG
SnaBI 596 TACGTA TAC^GTA
SpeI 120 ACTAGT A^CTAGT
Tsp45I 481 GTCAC ^GT[GC]AC
Tsp509I 515 AATT ^AATT
TspRI 654 CAGTG CA[GC]TG[ACGT][ACGT]^
XcmI 41 CCAAGAGGACAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 233 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AvaI 19 CTCGGG C^[CT]CG[GA]G
509 CCCGAG
BsaBI 196 GATCATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
228 GATCAGGATC
BsiYI 581 CCCTTGTACGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
582 CCTTGTACGGG
BslI 581 CCCTTGTACGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
582 CCTTGTACGGG
BsmFI 308 GGGAC GGGAC(10/14)
524 GTCCC
BsoBI 19 CTCGGG C^[CT]CG[GA]G
509 CCCGAG
DdeI 472 CTAAG C^T[ACGT]AG
641 CTAAG
EarI 150 GAAGAG CTCTTC(1/4)
663 GAAGAG
FauI 94 CCCGC CCCGC(4/6)
306 GCGGG
HphI 482 TCACC GGTGA(8/7)
676 TCACC
Ksp632I 150 GAAGAG CTCTTC(1/4)
663 GAAGAG
MaeII 417 ACGT A^CGT
597 ACGT
MamI 196 GATCATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
228 GATCAGGATC
PvuII 278 CAGCTG CAG^CTG
519 CAGCTG
SfaNI 126 GATGC GCATC(5/9)
217 GATGC
TaqI 366 TCGA T^CGA
408 TCGA
TthHB8I 366 TCGA T^CGA
408 TCGA
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# 3 OR MORE CUT ENZYMES ( 29 enzymes) #
#########################################
AciI AfaI AluI AlwI BbvI
BsaJI BsrI Cfr13I DpnII EcoT14I
Fnu4HI HaeIII HinfI ItaI MaeIII
MboI MboII MnlI MspA1I MwoI
NdeII NlaIII NlaIV RsaI Sau3AI
Sau96I StyI TfiI TseI