Restriction map for
pFN21AE1959
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# NO CUT ENZYMES ( 110 enzymes) #
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Acc65I AccIII AclI AcsI AflII
AhdI Alw44I AocI ApaI ApaLI
ApoI AscI AseI AsnI Asp718I
AspEI AviII AvrII BamHI BanII
BbrPI BbvCI BciVI BfrI BglI
BlnI BsaAI BsaBI BseAI BsgI
BsiEI BsiWI BsmBI Bsp1407I BspDI
BspEI BsrDI BsrGI BssHII Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
Bsu36I ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FspI HgaI HindIII HpaI
KpnI KspI MamI MfeI MluI
MroI MunI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RsrII SacI SacII
SapI SbfI ScaI SexAI SfiI
SfuI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI TfiI
Tsp509I XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AatII 220 GACGTC GACGT^C
AccI 1465 GTCGAC GT^[AC][GT]AC
AccII 1526 CGCG CG^CG
AfaI 993 GTAC GT^AC
AflIII 1515 ACATGT A^C[GA][CT]GT
AgeI 390 ACCGGT A^CCGGT
Aor51HI 209 AGCGCT AGC^GCT
Asp700I 1188 GAATGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 958 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 410 GGCGCC GGCGC^C
BbsI 1219 GTCTTC GAAGAC(2/6)
BcgI 1305 CGATGGCACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 515 TGATCA T^GATCA
BfaI 884 CTAG C^TAG
BglII 1400 AGATCT A^GATCT
BpuAI 1219 GTCTTC GAAGAC(2/6)
BsaI 1120 GAGACC GGTCTC(1/5)
BsaWI 390 ACCGGT [AT]^CCGG[AT]
BsmI 423 GAATGC GAATGC(1/-1)
BspHI 669 TCATGA T^CATGA
BspLU11I 1515 ACATGT A^CATGT
BssSI 655 CTCGTG CACGAG(-5/-1)
BstUI 1526 CGCG CG^CG
DsaI 133 CCATGG C^C[GA][CT]GG
EarI 1408 CTCTTC CTCTTC(1/4)
Eco47III 209 AGCGCT AGC^GCT
EcoT14I 133 CCATGG C^C[AT][AT]GG
FbaI 515 TGATCA T^GATCA
FseI 319 GGCCGGCC GGCCGG^CC
HinfI 382 GACTC G^A[ACGT]TC
KasI 410 GGCGCC G^GCGCC
Ksp632I 1408 CTCTTC CTCTTC(1/4)
MaeI 884 CTAG C^TAG
MseI 1319 TTAA T^TAA
MslI 246 CACGCCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 1526 CGCG CG^CG
NarI 410 GGCGCC GG^CGCC
NcoI 133 CCATGG C^CATGG
PflFI 958 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 974 CCATCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 390 ACCGGT A^CCGGT
PleI 382 GACTC GAGTC(4/5)
PvuII 1090 CAGCTG CAG^CTG
RcaI 669 TCATGA T^CATGA
RsaI 993 GTAC GT^AC
SalI 1465 GTCGAC G^TCGAC
SfoI 410 GGCGCC GGC^GCC
StyI 133 CCATGG C^C[AT][AT]GG
Tru9I 1319 TTAA T^TAA
Tth111I 958 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 974 CCATCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 1188 GAATGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 25 enzymes) #
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AcyI 220 GACGTC G[GA]^CG[CT]C
410 GGCGCC
AlwI 231 GATCC GGATC(4/5)
1026 GATCC
AspHI 79 GTGCTC G[AT]GC[AT]^C
808 GAGCAC
AvaI 98 CTCGGG C^[CT]CG[GA]G
151 CTCGGG
BlpI 261 GCTGAGC GC^T[ACGT]AGC
648 GCTCAGC
Bpu1102I 261 GCTGAGC GC^T[ACGT]AGC
648 GCTCAGC
BsaHI 220 GACGTC G[GA]^CG[CT]C
410 GGCGCC
BsiHKAI 79 GTGCTC G[AT]GC[AT]^C
808 GAGCAC
BsmAI 682 GAGAC GTCTC(1/5)
1120 GAGAC
BsoBI 98 CTCGGG C^[CT]CG[GA]G
151 CTCGGG
BsrBI 1060 GAGCGG CCGCTC(-3/-3)
1288 GAGCGG
CelII 261 GCTGAGC GC^T[ACGT]AGC
648 GCTCAGC
Hin1I 220 GACGTC G[GA]^CG[CT]C
410 GGCGCC
MaeII 221 ACGT A^CGT
593 ACGT
MaeIII 964 GTCAC ^GT[ACGT]AC
1000 GTGAC
MwoI 418 GCCCAGAATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
1065 GCGGCCCCGGC
NaeI 320 GCCGGC GCC^GGC
407 GCCGGC
NgoMIV 320 GCCGGC G^CCGGC
407 GCCGGC
PpuMI 101 GGGTCCC [GA]G^G[AT]CC[CT]
1265 AGGACCC
SfaNI 645 GATGC GCATC(5/9)
1344 GATGC
SgrAI 406 CGCCGGCG C[GA]^CCGG[CT]G
1366 CGCCGGTG
SmlI 82 CTCAAG C^T[CT][GA]AG
1329 CTTGAG
TaqI 557 TCGA T^CGA
1466 TCGA
Tsp45I 964 GTCAC ^GT[GC]AC
1000 GTGAC
TthHB8I 557 TCGA T^CGA
1466 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AciI AluI AlwNI AvaII BalI
BanI BbvI BcnI BmyI BpmI
BsaJI BseRI BsiYI BslI BsmFI
Bsp1286I BspMI BsrFI BsrI BssKI
BstF5I BstNI BstXI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII EaeI EcoO109I EcoRII FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HincII HindII HpaII
HphI ItaI MboI MboII MflI
MluNI MnlI MscI MspA1I MspI
MvaI NciI NdeII NlaIII NlaIV
NspI PstI Sau3AI Sau96I ScrFI
SfcI StuI TseI TspRI XcmI
XhoII