Restriction map for
pFN21AE3258
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# NO CUT ENZYMES ( 125 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AgeI AhdI Alw44I
Aor51HI ApaLI AscI Asp718I AspEI
AspHI AspI AviII AvrII BamHI
BanI BbeI BbrPI BbvCI BcgI
BciVI BglI BlnI BlpI BpmI
Bpu1102I BsaAI BsaHI BsaI BseAI
BsiEI BsiHKAI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstUI BstZ17I CelII CfoI
Cfr10I ClaI CpoI DrdI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
EcoT22I FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HpaI KasI
KpnI KspI MfeI MluI MroI
MunI MvnI NaeI NarI NcoI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshAI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI SspBI SspI StuI SwaI
Tth111I XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AciI 1146 GCGG CCGC(-3/-1)
AflII 481 CTTAAG C^TTAAG
AflIII 1455 ACATGT A^C[GA][CT]GT
AlwNI 1184 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 123 CCTCAGG CC^T[ACGT]AGG
ApaI 1714 GGGCCC GGGCC^C
AseI 1555 ATTAAT AT^TAAT
AsnI 1555 ATTAAT AT^TAAT
Asp700I 877 GAAGGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 298 CCCGAG C^[CT]CG[GA]G
AvaII 628 GGACC G^G[AT]CC
BclI 164 TGATCA T^GATCA
BcnI 1689 CCCGG CC^[GC]GG
BfrI 481 CTTAAG C^TTAAG
BsaWI 1140 ACCGGA [AT]^CCGG[AT]
BsoBI 298 CCCGAG C^[CT]CG[GA]G
BspLU11I 1455 ACATGT A^CATGT
BspMI 1607 ACCTGC ACCTGC(4/8)
BsrBI 1144 GAGCGG CCGCTC(-3/-3)
BstEII 1603 GGTGACC G^GT[ACGT]ACC
BstPI 1603 GGTGACC G^GT[ACGT]ACC
BstXI 18 CCACTGGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 123 CCTCAGG CC^T[ACGT]AGG
Eco81I 123 CCTCAGG CC^T[ACGT]AGG
EcoNI 1582 CCTGGTCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1603 GGTGACC G^GT[ACGT]ACC
EcoRV 694 GATATC GAT^ATC
FauI 1146 GCGGG CCCGC(4/6)
FbaI 164 TGATCA T^GATCA
HincII 1165 GTTGAC GT[CT]^[GA]AC
HindII 1165 GTTGAC GT[CT]^[GA]AC
MslI 458 CACAGCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NciI 1689 CCCGG CC^[GC]GG
NdeI 1478 CATATG CA^TATG
PflMI 606 CCAAGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 774 GAGTC GAGTC(4/5)
PshBI 1555 ATTAAT AT^TAAT
SbfI 1608 CCTGCAGG CCTGCA^GG
SexAI 1254 ACCTGGT A^CC[AT]GGT
Sse8387I 1608 CCTGCAGG CCTGCA^GG
Tsp45I 1604 GTGAC ^GT[GC]AC
Van91I 606 CCAAGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1417 TCTAGA T^CTAGA
XmnI 877 GAAGGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AlwI 1129 GGATC GGATC(4/5)
1500 GGATC
BalI 466 TGGCCA TGG^CCA
1523 TGGCCA
BanII 1288 GGGCTC G[GA]GC[CT]^C
1714 GGGCCC
BglII 1414 AGATCT A^GATCT
1761 AGATCT
BmyI 1288 GGGCTC G[AGT]GC[ACT]^C
1714 GGGCCC
BsgI 380 CTGCAC GTGCAG(16/14)
1331 GTGCAG
BsmFI 1267 GGGAC GGGAC(10/14)
256 GTCCC
Bsp1286I 1288 GGGCTC G[AGT]GC[ACT]^C
1714 GGGCCC
BsrDI 714 CATTGC GCAATG(2/0)
1837 GCAATG
BstYI 1414 AGATCT [GA]^GATC[CT]
1761 AGATCT
Cac8I 1632 GCAGGC GC[ACGT]^[ACGT]GC
1683 GCTGGC
DraI 535 TTTAAA TTT^AAA
1308 TTTAAA
DraIII 311 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG
1253 CACCTGGTG
EaeI 466 TGGCCA [CT]^GGCC[GA]
1523 TGGCCA
EcoRI 411 GAATTC G^AATTC
516 GAATTC
EcoT14I 243 CCAAGG C^C[AT][AT]GG
1390 CCAAGG
HapII 1141 CCGG C^CGG
1690 CCGG
HindIII 347 AAGCTT A^AGCTT
478 AAGCTT
HpaII 1141 CCGG C^CGG
1690 CCGG
MaeII 645 ACGT A^CGT
1348 ACGT
MaeIII 975 GTTAC ^GT[ACGT]AC
1604 GTGAC
MflI 1414 AGATCT [GA]^GATC[CT]
1761 AGATCT
MluNI 466 TGGCCA TGG^CCA
1523 TGGCCA
MscI 466 TGGCCA TGG^CCA
1523 TGGCCA
MspA1I 100 CAGCTG C[AC]G^C[GT]G
1048 CAGCTG
MspI 1141 CCGG C^CGG
1690 CCGG
NspI 488 ACATGC [GA]CATG^[CT]
1455 ACATGT
PstI 959 CTGCAG CTGCA^G
1609 CTGCAG
PvuII 100 CAGCTG CAG^CTG
1048 CAGCTG
ScaI 1517 AGTACT AGT^ACT
1748 AGTACT
SfcI 959 CTGCAG C^T[GA][CT]AG
1609 CTGCAG
SmlI 481 CTTAAG C^T[CT][GA]AG
1062 CTCAAG
StyI 243 CCAAGG C^C[AT][AT]GG
1390 CCAAGG
TaqI 344 TCGA T^CGA
1423 TCGA
TthHB8I 344 TCGA T^CGA
1423 TCGA
XhoII 1414 AGATCT [GA]^GATC[CT]
1761 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AcsI AfaI AluI ApoI BbsI
BbvI BfaI BpuAI BsaBI BsaJI
BseRI BsiYI BslI BsmAI BsrI
BssKI BstF5I BstNI Cfr13I DdeI
DpnII DraII EarI EcoO109I EcoRII
Fnu4HI FokI HaeIII HinfI HphI
ItaI Ksp632I MaeI MamI MboI
MboII MnlI MseI MvaI MwoI
NdeII NlaIII NlaIV RsaI Sau3AI
Sau96I ScrFI SfaNI TfiI Tru9I
TseI Tsp509I TspRI