Restriction map for
pFN21AE1690
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# NO CUT ENZYMES ( 170 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI ApoI AscI Asp700I
Asp718I AspEI AspHI AspI AvaI
AviII AvrII BanI BanII BbeI
BbvCI BcgI BciVI BcnI BfrI
BglI BglII BlnI BlpI BmyI
BpmI Bpu1102I BsaBI BsaHI BsaWI
BseAI BsgI BsiEI BsiHKAI BsiWI
BsmBI BsoBI Bsp1286I Bsp1407I BspDI
BspEI BspHI BspLU11I BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I CelII CfoI Cfr10I ClaI
CpoI DraI DraIII DsaI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FseI FspI HaeII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HpaI HpaII KasI
KpnI Ksp632I KspI MamI MfeI
MluI MroI MslI MspI MunI
MvnI NaeI NarI NciI NcoI
NdeI NgoMIV NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PmeI PshAI Psp1406I PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI StyI SwaI
TaqI Tsp509I Tth111I TthHB8I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AfaI 242 GTAC GT^AC
AlwNI 124 CAGGGCCTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 430 ATTAAT AT^TAAT
AsnI 430 ATTAAT AT^TAAT
BalI 260 TGGCCA TGG^CCA
BamHI 554 GGATCC G^GATCC
BbrPI 563 CACGTG CAC^GTG
BbsI 274 GAAGAC GAAGAC(2/6)
BclI 183 TGATCA T^GATCA
BfaI 67 CTAG C^TAG
BpuAI 274 GAAGAC GAAGAC(2/6)
BsaAI 563 CACGTG [CT]AC^GT[GA]
BsaI 448 GGTCTC GGTCTC(1/5)
BsaJI 203 CCGAGG C^C[ACGT][ACGT]GG
BseRI 370 GAGGAG GAGGAG(10/8)
BsmAI 449 GTCTC GTCTC(1/5)
BsmFI 41 GGGAC GGGAC(10/14)
BsmI 15 GCATTC GAATGC(1/-1)
BspMI 282 GCAGGT ACCTGC(4/8)
BsrBI 54 CCGCTC CCGCTC(-3/-3)
BsrDI 407 GCAATG GCAATG(2/0)
DdeI 270 CTTAG C^T[ACGT]AG
DrdI 504 GACAGTAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 260 TGGCCA [CT]^GGCC[GA]
FauI 404 CCCGC CCCGC(4/6)
FbaI 183 TGATCA T^GATCA
HindIII 233 AAGCTT A^AGCTT
MaeI 67 CTAG C^TAG
MaeII 564 ACGT A^CGT
MaeIII 308 GTGAC ^GT[ACGT]AC
MluNI 260 TGGCCA TGG^CCA
MscI 260 TGGCCA TGG^CCA
MspA1I 22 CCGCTG C[AC]G^C[GT]G
NheI 66 GCTAGC G^CTAGC
PflMI 559 CCAGCACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 563 CACGTG CAC^GTG
PmlI 563 CACGTG CAC^GTG
PpuMI 41 GGGACCT [GA]G^G[AT]CC[CT]
PshBI 430 ATTAAT AT^TAAT
PstI 484 CTGCAG CTGCA^G
RsaI 242 GTAC GT^AC
Tsp45I 308 GTGAC ^GT[GC]AC
Van91I 559 CCAGCACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AvaII 42 GGACC G^G[AT]CC
512 GGTCC
BbvI 349 GCTGC GCAGC(8/12)
483 GCTGC
BsrI 550 ACTGG ACTGG(1/-1)
169 CCAGT
BstYI 398 AGATCC [GA]^GATC[CT]
554 GGATCC
Cac8I 66 GCTAGC GC[ACGT]^[ACGT]GC
175 GCAAGC
MflI 398 AGATCC [GA]^GATC[CT]
554 GGATCC
MseI 376 TTAA T^TAA
431 TTAA
NlaIII 250 CATG CATG^
520 CATG
PleI 151 GACTC GAGTC(4/5)
215 GACTC
SfcI 48 CTGTAG C^T[GA][CT]AG
484 CTGCAG
TfiI 78 GATTC G^A[AT]TC
106 GAATC
Tru9I 376 TTAA T^TAA
431 TTAA
TseI 349 GCTGC G^C[AT]GC
483 GCTGC
XhoII 398 AGATCC [GA]^GATC[CT]
554 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 31 enzymes) #
#########################################
AciI AluI AlwI BsiYI BslI
BssKI BstF5I BstNI Cfr13I DpnII
DraII EcoO109I EcoRII Fnu4HI FokI
HaeIII HinfI HphI ItaI MboI
MboII MnlI MvaI MwoI NdeII
NlaIV Sau3AI Sau96I ScrFI SfaNI
TspRI