Restriction map for
pFN21AE9621
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# NO CUT ENZYMES ( 147 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspI AvaI AviII AvrII
BamHI BanI BbeI BbrPI BcgI
BcnI BfrI BglI BglII BlnI
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BsgI BsiEI BsiWI BsmBI
BsoBI Bsp1407I BspDI BspEI BspMI
BsrBI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I CfoI Cfr10I
ClaI CpoI DraII DraIII DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRV FauI FseI FspI HaeII
HapII HgaI HhaI Hin1I HinP1I
HindIII HpaI HpaII KasI KpnI
KspI MaeII MamI MluI MroI
MspI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
StuI SwaI Tth111I XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AciI 955 GCGG CCGC(-3/-1)
AfaI 772 GTAC GT^AC
AflIII 12 ACATGT A^C[GA][CT]GT
BalI 41 TGGCCA TGG^CCA
BanII 913 GAGCTC G[GA]GC[CT]^C
BbvCI 1339 GCTGAGG CCTCAGC(-5/-2)
BciVI 1281 GGATAC GTATCC(6/5)
BclI 663 TGATCA T^GATCA
BfaI 109 CTAG C^TAG
BlpI 1605 GCTGAGC GC^T[ACGT]AGC
BpmI 575 CTGGAG CTGGAG(16/14)
Bpu1102I 1605 GCTGAGC GC^T[ACGT]AGC
BsmFI 1647 GGGAC GGGAC(10/14)
BspLU11I 12 ACATGT A^CATGT
CelII 1605 GCTGAGC GC^T[ACGT]AGC
DraI 1114 TTTAAA TTT^AAA
EaeI 41 TGGCCA [CT]^GGCC[GA]
EcoRI 531 GAATTC G^AATTC
EcoT14I 140 CCAAGG C^C[AT][AT]GG
EcoT22I 688 ATGCAT ATGCA^T
FbaI 663 TGATCA T^GATCA
MaeI 109 CTAG C^TAG
MaeIII 1538 GTGAC ^GT[ACGT]AC
MfeI 842 CAATTG C^AATTG
MluNI 41 TGGCCA TGG^CCA
MscI 41 TGGCCA TGG^CCA
MunI 842 CAATTG C^AATTG
NlaIV 1647 GGGACC GG[ACGT]^[ACGT]CC
NsiI 688 ATGCAT ATGCA^T
PflMI 44 CCAAAATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 629 CTGCAG CTGCA^G
RsaI 772 GTAC GT^AC
SacI 913 GAGCTC GAGCT^C
StyI 140 CCAAGG C^C[AT][AT]GG
Tsp45I 1538 GTGAC ^GT[GC]AC
Van91I 44 CCAAAATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 108 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 41 enzymes) #
###################################
AspHI 913 GAGCTC G[AT]GC[AT]^C
1616 GTGCTC
AvaII 541 GGTCC G^G[AT]CC
1648 GGACC
BbsI 497 GAAGAC GAAGAC(2/6)
682 GAAGAC
BmyI 913 GAGCTC G[AGT]GC[ACT]^C
1616 GTGCTC
BpuAI 497 GAAGAC GAAGAC(2/6)
682 GAAGAC
BseRI 885 GAGGAG GAGGAG(10/8)
1022 GAGGAG
BsiHKAI 913 GAGCTC G[AT]GC[AT]^C
1616 GTGCTC
BsmAI 1183 GAGAC GTCTC(1/5)
1432 GAGAC
BsmI 366 GAATGC GAATGC(1/-1)
1499 GAATGC
Bsp1286I 913 GAGCTC G[AGT]GC[ACT]^C
1616 GTGCTC
BspHI 342 TCATGA T^CATGA
1275 TCATGA
BssKI 271 CCAGG ^CC[ACGT]GG
1053 CCAGG
BstBI 158 TTCGAA TT^CGAA
191 TTCGAA
BstF5I 1187 CATCC GGATG(2/0)
1245 GGATG
BstNI 271 CCAGG CC^[AT]GG
1053 CCAGG
BstYI 900 AGATCC [GA]^GATC[CT]
1258 GGATCT
Cac8I 54 GCTTGC GC[ACGT]^[ACGT]GC
1593 GCTGGC
EarI 817 GAAGAG CTCTTC(1/4)
1014 GAAGAG
EcoRII 271 CCAGG ^CC[AT]GG
1053 CCAGG
FokI 1187 CATCC GGATG(9/13)
1245 GGATG
HincII 280 GTTGAC GT[CT]^[GA]AC
1041 GTCAAC
HindII 280 GTTGAC GT[CT]^[GA]AC
1041 GTCAAC
HphI 144 GGTGA GGTGA(8/7)
136 TCACC
Ksp632I 817 GAAGAG CTCTTC(1/4)
1014 GAAGAG
MflI 900 AGATCC [GA]^GATC[CT]
1258 GGATCT
MslI 9 CACACATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
960 CACACTCATG
MspA1I 1553 CAGCTG C[AC]G^C[GT]G
1591 CAGCTG
MvaI 271 CCAGG CC^[AT]GG
1053 CCAGG
MwoI 714 GCCTTCTGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
1618 GCTCCCTATGC
NspI 12 ACATGT [GA]CATG^[CT]
686 ACATGC
NspV 158 TTCGAA TT^CGAA
191 TTCGAA
PleI 1144 GAGTC GAGTC(4/5)
1405 GAGTC
PvuII 1553 CAGCTG CAG^CTG
1591 CAGCTG
RcaI 342 TCATGA T^CATGA
1275 TCATGA
ScrFI 271 CCAGG CC^[ACGT]GG
1053 CCAGG
SfaNI 1194 GATGC GCATC(5/9)
307 GCATC
SfcI 629 CTGCAG C^T[GA][CT]AG
1527 CTGTAG
SfuI 158 TTCGAA TT^CGAA
191 TTCGAA
TaqI 159 TCGA T^CGA
192 TCGA
TthHB8I 159 TCGA T^CGA
192 TCGA
XhoII 900 AGATCC [GA]^GATC[CT]
1258 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 33 enzymes) #
#########################################
AcsI AluI AlwI ApoI Asp700I
BbvI BsaJI BsiYI BslI BsrDI
BsrI Cfr13I DdeI DpnII Fnu4HI
HaeIII HinfI ItaI MboI MboII
MnlI MseI NdeII NlaIII Sau3AI
Sau96I SspI TfiI Tru9I TseI
Tsp509I TspRI XmnI