Restriction map for
pFN21AE9516
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# NO CUT ENZYMES ( 89 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI Alw44I
Aor51HI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AviII AvrII
BbrPI BbsI BbvCI BciVI BfrI
BglII BlnI BpuAI BsaAI BsaBI
BseAI BsiWI Bsp1407I BspDI BspEI
BspLU11I BsrGI BssHII Bst1107I BstZ17I
ClaI CpoI DraI DraIII Eam1105I
Eco47III EcoNI EcoT22I FseI FspI
HindIII HpaI KpnI KspI MamI
MfeI MluI MroI MunI NcoI
NdeI NheI NotI NruI NsiI
NspI PacI PaeR7I PinAI PmaCI
PmeI PmlI PshBI Psp1406I RsrII
SacII SalI SexAI SfiI SgrAI
SnaBI SpeI SphI SspBI SspI
SwaI XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AocI 2202 CCTCAGG CC^T[ACGT]AGG
ApaI 2198 GGGCCC GGGCC^C
AspHI 1627 GAGCTC G[AT]GC[AT]^C
BcgI 205 GCAACACCGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1367 TGATCA T^GATCA
BfaI 2128 CTAG C^TAG
BlpI 596 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 596 GCTCAGC GC^T[ACGT]AGC
BsaI 1154 GAGACC GGTCTC(1/5)
BsaWI 2111 ACCGGA [AT]^CCGG[AT]
BsiHKAI 1627 GAGCTC G[AT]GC[AT]^C
BspHI 731 TCATGA T^CATGA
BsrBI 817 GAGCGG CCGCTC(-3/-3)
BsrFI 1516 GCCGGC [GA]^CCGG[CT]
BssSI 102 CTCGTG CACGAG(-5/-1)
BstBI 786 TTCGAA TT^CGAA
BstEII 606 GGTCACC G^GT[ACGT]ACC
BstPI 606 GGTCACC G^GT[ACGT]ACC
BstXI 1505 CCATCTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 2202 CCTCAGG CC^T[ACGT]AGG
CelII 596 GCTCAGC GC^T[ACGT]AGC
Cfr10I 1516 GCCGGC [GA]^CCGG[CT]
DrdI 826 GACAAGAACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1755 CGGCCG C^GGCCG
EclXI 1755 CGGCCG C^GGCCG
Eco52I 1755 CGGCCG C^GGCCG
Eco81I 2202 CCTCAGG CC^T[ACGT]AGG
EcoO65I 606 GGTCACC G^GT[ACGT]ACC
EcoRI 2155 GAATTC G^AATTC
EcoRV 727 GATATC GAT^ATC
FbaI 1367 TGATCA T^GATCA
HincII 1054 GTCAAC GT[CT]^[GA]AC
HindII 1054 GTCAAC GT[CT]^[GA]AC
MaeI 2128 CTAG C^TAG
MseI 1915 TTAA T^TAA
NaeI 1516 GCCGGC GCC^GGC
NgoMIV 1516 GCCGGC G^CCGGC
NspV 786 TTCGAA TT^CGAA
PshAI 1857 GACTACCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 2050 CTGCAG CTGCA^G
PvuI 2004 CGATCG CGAT^CG
RcaI 731 TCATGA T^CATGA
SacI 1627 GAGCTC GAGCT^C
SapI 1956 GCTCTTC GCTCTTC(1/4)
SbfI 2049 CCTGCAGG CCTGCA^GG
ScaI 2147 AGTACT AGT^ACT
SfuI 786 TTCGAA TT^CGAA
SmlI 1126 CTTGAG C^T[CT][GA]AG
Sse8387I 2049 CCTGCAGG CCTGCA^GG
StuI 1620 AGGCCT AGG^CCT
Tru9I 1915 TTAA T^TAA
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AcsI 232 AAATTC [GA]^AATT[CT]
2155 GAATTC
AfaI 1673 GTAC GT^AC
2148 GTAC
ApoI 232 AAATTC [GA]^AATT[CT]
2155 GAATTC
AspI 478 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
559 GACAAAGTC
AvaI 282 CCCGGG C^[CT]CG[GA]G
2195 CCCGGG
BamHI 737 GGATCC G^GATCC
897 GGATCC
BbeI 675 GGCGCC GGCGC^C
2056 GGCGCC
BseRI 99 CTCCTC GAGGAG(10/8)
1000 GAGGAG
BsiEI 1755 CGGCCG CG[GA][CT]^CG
2004 CGATCG
BsmBI 1725 CGTCTC CGTCTC(1/5)
659 GAGACG
BsmI 22 GCATTC GAATGC(1/-1)
975 GCATTC
BsoBI 282 CCCGGG C^[CT]CG[GA]G
2195 CCCGGG
BsrDI 947 CATTGC GCAATG(2/0)
1838 GCAATG
BstYI 737 GGATCC [GA]^GATC[CT]
897 GGATCC
KasI 675 GGCGCC G^GCGCC
2056 GGCGCC
MflI 737 GGATCC [GA]^GATC[CT]
897 GGATCC
NarI 675 GGCGCC GG^CGCC
2056 GGCGCC
PflFI 478 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
559 GACAAAGTC
PflMI 567 CCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1260 CCAGGTGCTGG
PpuMI 569 AGGACCT [GA]G^G[AT]CC[CT]
1812 GGGACCT
RsaI 1673 GTAC GT^AC
2148 GTAC
SfcI 1740 CTACAG C^T[GA][CT]AG
2050 CTGCAG
SfoI 675 GGCGCC GGC^GCC
2056 GGCGCC
SmaI 282 CCCGGG CCC^GGG
2195 CCCGGG
TfiI 458 GAATC G^A[AT]TC
551 GATTC
Tth111I 478 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
559 GACAAAGTC
Van91I 567 CCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1260 CCAGGTGCTGG
XcmI 1373 CCAGGGTGGGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1502 CCACCATCTTCCTGG
XhoII 737 GGATCC [GA]^GATC[CT]
897 GGATCC
XmaCI 282 CCCGGG C^CCGGG
2195 CCCGGG
XmaI 282 CCCGGG C^CCGGG
2195 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 87 enzymes) #
#########################################
AccII AciI AcyI AluI AlwI
AlwNI AvaII BalI BanI BanII
BbvI BcnI BglI BmyI BpmI
BsaHI BsaJI BsgI BsiYI BslI
BsmAI BsmFI Bsp1286I BspMI BsrI
BssKI BstAPI BstF5I BstNI BstUI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeII MaeIII MboI
MboII MluNI MnlI MscI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PleI
PvuII Sau3AI Sau96I ScrFI SfaNI
StyI TaqI TseI Tsp45I Tsp509I
TspRI TthHB8I