Restriction map for
pFN21AE4073
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# NO CUT ENZYMES ( 111 enzymes) #
##################################
Acc65I AccI AclI AcsI AflII
AgeI AhdI Alw44I AocI ApaLI
ApoI AseI AsnI Asp718I AspEI
AviII AvrII BamHI BbrPI BbsI
BbvCI BcgI BciVI BclI BfrI
BglII BlnI BlpI Bpu1102I BpuAI
BsmBI BsmI Bsp1407I BspDI BspHI
BspLU11I BsrDI BsrGI Bst1107I BstBI
BstEII BstPI BstZ17I Bsu36I CelII
ClaI CpoI DraI DrdI Eam1105I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HincII
HindII HindIII HpaI KpnI MfeI
MluI MseI MunI NdeI NruI
NsiI NspI NspV PacI PaeR7I
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsrII SalI SapI SbfI
ScaI SfiI SfuI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TaqI Tru9I Tsp509I
TthHB8I XbaI XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AatII 106 GACGTC GACGT^C
AccIII 995 TCCGGA T^CCGGA
AfaI 837 GTAC GT^AC
AflIII 839 ACGTGT A^C[GA][CT]GT
AlwI 1011 GATCC GGATC(4/5)
AlwNI 1041 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 386 AGCGCT AGC^GCT
Asp700I 858 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 103 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 985 GGCGCC GGCGC^C
BfaI 644 CTAG C^TAG
BglI 168 GCCTCCCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 838 TACGTG [CT]AC^GT[GA]
BsaBI 696 GATCGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 23 GGTCTC GGTCTC(1/5)
BsaWI 995 TCCGGA [AT]^CCGG[AT]
BseAI 995 TCCGGA T^CCGGA
BsiWI 836 CGTACG C^GTACG
BspEI 995 TCCGGA T^CCGGA
BsrBI 271 GAGCGG CCGCTC(-3/-3)
BssSI 67 CACGAG CACGAG(-5/-1)
BstYI 1010 AGATCC [GA]^GATC[CT]
EarI 670 GAAGAG CTCTTC(1/4)
Eco47III 386 AGCGCT AGC^GCT
HinfI 40 GATTC G^A[ACGT]TC
HphI 585 TCACC GGTGA(8/7)
KasI 985 GGCGCC G^GCGCC
Ksp632I 670 GAAGAG CTCTTC(1/4)
MaeI 644 CTAG C^TAG
MamI 696 GATCGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 1010 AGATCC [GA]^GATC[CT]
MroI 995 TCCGGA T^CCGGA
NarI 985 GGCGCC GG^CGCC
NcoI 773 CCATGG C^CATGG
NheI 643 GCTAGC G^CTAGC
PflFI 103 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 1038 CTGCAG CTGCA^G
RsaI 837 GTAC GT^AC
SexAI 323 ACCTGGT A^CC[AT]GGT
SfcI 1038 CTGCAG C^T[GA][CT]AG
SfoI 985 GGCGCC GGC^GCC
SmlI 523 CTCAAG C^T[CT][GA]AG
TfiI 40 GATTC G^A[AT]TC
Tth111I 103 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 1010 AGATCC [GA]^GATC[CT]
XmnI 858 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AcyI 106 GACGTC G[GA]^CG[CT]C
985 GGCGCC
AspHI 315 GAGCTC G[AT]GC[AT]^C
399 GAGCTC
AvaI 919 CTCGGG C^[CT]CG[GA]G
1120 CCCGAG
AvaII 48 GGACC G^G[AT]CC
233 GGTCC
BalI 59 TGGCCA TGG^CCA
494 TGGCCA
BpmI 396 CTGGAG CTGGAG(16/14)
847 CTGGAG
BsaHI 106 GACGTC G[GA]^CG[CT]C
985 GGCGCC
BsiHKAI 315 GAGCTC G[AT]GC[AT]^C
399 GAGCTC
BsmAI 999 GAGAC GTCTC(1/5)
24 GTCTC
BsoBI 919 CTCGGG C^[CT]CG[GA]G
1120 CCCGAG
BspMI 451 ACCTGC ACCTGC(4/8)
587 ACCTGC
BstAPI 321 GCACCTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1040 GCAGCAGCTGC
BstXI 629 CCAAGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
701 CCATCCCCGTGG
DdeI 44 CTGAG C^T[ACGT]AG
479 CTCAG
DpnII 696 GATC ^GATC
1011 GATC
DraIII 322 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
1015 CACGCGGTG
Hin1I 106 GACGTC G[GA]^CG[CT]C
985 GGCGCC
MaeII 107 ACGT A^CGT
839 ACGT
MaeIII 65 GTCAC ^GT[ACGT]AC
584 GTCAC
MboI 696 GATC ^GATC
1011 GATC
MluNI 59 TGGCCA TGG^CCA
494 TGGCCA
MscI 59 TGGCCA TGG^CCA
494 TGGCCA
NaeI 160 GCCGGC GCC^GGC
942 GCCGGC
NdeII 696 GATC ^GATC
1011 GATC
NgoMIV 160 GCCGGC G^CCGGC
942 GCCGGC
PflMI 51 CCAGCAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1014 CCACGCGGTGG
SacI 315 GAGCTC GAGCT^C
399 GAGCTC
Sau3AI 696 GATC ^GATC
1011 GATC
Tsp45I 65 GTCAC ^GT[GC]AC
584 GTCAC
TspRI 77 CAGTG CA[GC]TG[ACGT][ACGT]^
229 CACTG
Van91I 51 CCAGCAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1014 CCACGCGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
#########################################
AccII AciI AluI ApaI AscI
BanI BanII BbvI BcnI BmyI
BsaJI BseRI BsgI BsiEI BsiYI
BslI BsmFI Bsp1286I BsrFI BsrI
BssHII BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr10I Cfr13I DraII
DsaI EaeI EagI EclXI Eco52I
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HpaII ItaI KspI
MboII MnlI MslI MspA1I MspI
MvaI MvnI MwoI NciI NlaIII
NlaIV NotI PvuII SacII Sau96I
ScrFI SfaNI SgrAI StyI TseI