Restriction map for
pFN21AE2090
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# NO CUT ENZYMES ( 144 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AhdI Alw44I Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspHI AspI AvaI AvaII
AviII AvrII BamHI BanII BbeI
BbrPI BbvCI BcgI BciVI BclI
BcnI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaHI BseAI BseRI
BsiEI BsiHKAI BsiWI BsmBI BsoBI
Bsp1407I BspEI BspHI BspLU11I BsrBI
BsrGI BssHII BssKI Bst1107I BstAPI
BstBI BstEII BstNI BstPI BstZ17I
CelII CfoI Cfr13I CpoI DraII
DraIII EagI Eam1105I EclXI Eco47III
Eco52I EcoO109I EcoO65I EcoRII EcoRV
EcoT22I FbaI FseI FspI HaeII
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI KasI KpnI KspI
MamI MluI MroI MspA1I MvaI
NaeI NarI NciI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacI SacII SalI Sau96I SbfI
ScaI ScrFI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SphI
Sse8387I SspBI SwaI Tth111I Van91I
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AciI 35 CCGC CCGC(-3/-1)
AflII 2242 CTTAAG C^TTAAG
AflIII 1181 ACACGT A^C[GA][CT]GT
AgeI 1981 ACCGGT A^CCGGT
AocI 650 CCTCAGG CC^T[ACGT]AGG
BalI 1310 TGGCCA TGG^CCA
BanI 1630 GGTGCC G^G[CT][GA]CC
BfrI 2242 CTTAAG C^TTAAG
BglI 537 GCCTTCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI 1631 GTGCCC G[AGT]GC[ACT]^C
BsaI 1580 GAGACC GGTCTC(1/5)
BsgI 613 GTGCAG GTGCAG(16/14)
Bsp1286I 1631 GTGCCC G[AGT]GC[ACT]^C
BspDI 1018 ATCGAT AT^CGAT
BspMI 2247 GCAGGT ACCTGC(4/8)
BsrDI 599 CATTGC GCAATG(2/0)
BsrFI 1981 ACCGGT [GA]^CCGG[CT]
BssSI 2043 CACGAG CACGAG(-5/-1)
BstF5I 111 GGATG GGATG(2/0)
BstXI 862 CCAGTGAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1595 AGATCC [GA]^GATC[CT]
Bsu36I 650 CCTCAGG CC^T[ACGT]AGG
Cfr10I 1981 ACCGGT [GA]^CCGG[CT]
ClaI 1018 ATCGAT AT^CGAT
DrdI 516 GACTGTGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1307 CCATGG C^C[GA][CT]GG
EaeI 1310 TGGCCA [CT]^GGCC[GA]
Eco81I 650 CCTCAGG CC^T[ACGT]AGG
EcoNI 1725 CCTTACTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 34 CCCGC CCCGC(4/6)
FokI 111 GGATG GGATG(9/13)
MaeII 1183 ACGT A^CGT
MflI 1595 AGATCC [GA]^GATC[CT]
MluNI 1310 TGGCCA TGG^CCA
MscI 1310 TGGCCA TGG^CCA
MslI 1562 CACAACAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 1307 CCATGG C^CATGG
NdeI 1372 CATATG CA^TATG
NspI 80 GCATGT [GA]CATG^[CT]
PinAI 1981 ACCGGT A^CCGGT
SapI 257 GCTCTTC GCTCTTC(1/4)
SpeI 171 ACTAGT A^CTAGT
SspI 1237 AATATT AAT^ATT
StuI 665 AGGCCT AGG^CCT
XcmI 1767 CCATTTGACCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 1595 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 22 enzymes) #
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AccII 62 CGCG CG^CG
1034 CGCG
AlwNI 512 CAGTGACTG CAG[ACGT][ACGT][ACGT]^CTG
2248 CAGGTGCTG
Asp700I 377 GAAATTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2212 GAAACCATTC
BsaJI 1307 CCATGG C^C[ACGT][ACGT]GG
1635 CCAAGG
BsaWI 1981 ACCGGT [AT]^CCGG[AT]
2199 ACCGGA
BsmFI 1030 GGGAC GGGAC(10/14)
1111 GGGAC
BsmI 1941 GAATGC GAATGC(1/-1)
846 GCATTC
BstUI 62 CGCG CG^CG
1034 CGCG
Cac8I 297 GCCAGC GC[ACGT]^[ACGT]GC
1299 GCTTGC
DraI 951 TTTAAA TTT^AAA
1708 TTTAAA
EcoT14I 1307 CCATGG C^C[AT][AT]GG
1635 CCAAGG
HapII 1982 CCGG C^CGG
2200 CCGG
HgaI 1032 GACGC GACGC(5/10)
592 GCGTC
HpaII 1982 CCGG C^CGG
2200 CCGG
MfeI 280 CAATTG C^AATTG
829 CAATTG
MspI 1982 CCGG C^CGG
2200 CCGG
MunI 280 CAATTG C^AATTG
829 CAATTG
MvnI 62 CGCG CG^CG
1034 CGCG
NlaIV 1630 GGTGCC GG[ACGT]^[ACGT]CC
1984 GGTTCC
SfcI 818 CTACAG C^T[GA][CT]AG
889 CTACAG
StyI 1307 CCATGG C^C[AT][AT]GG
1635 CCAAGG
XmnI 377 GAAATTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2212 GAAACCATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
BbsI BbvI BfaI BpmI BpuAI
BsiYI BslI BsmAI BsrI DdeI
DpnII EarI EcoRI Fnu4HI HaeIII
HinfI HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MnlI MseI
MwoI NdeII NlaIII PleI RsaI
Sau3AI SfaNI SmlI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I