Restriction map for
pFN21AE3296
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# NO CUT ENZYMES ( 101 enzymes) #
##################################
AccI AccII AccIII AclI AflII
AgeI Aor51HI ApaI AscI AseI
AsnI Asp700I AvaI AviII AvrII
BamHI BbeI BcgI BciVI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaWI BseAI BsiEI BsiWI BsmBI
BsoBI BspEI BsrBI BssHII Bst1107I
BstBI BstEII BstPI BstUI BstXI
BstZ17I CelII CpoI DraI DraIII
EagI EclXI Eco47III Eco52I EcoO65I
FauI FseI FspI HaeII HgaI
HindIII HpaI KasI KspI MfeI
MluI MroI MunI MvnI NaeI
NarI NcoI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PinAI
PmeI PshAI PshBI Psp1406I PvuI
RsrII SacI SacII SalI SbfI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI Sse8387I SspI
SwaI XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 60 enzymes) #
###################################
AatII 1616 GACGTC GACGT^C
Acc65I 144 GGTACC G^GTACC
AcyI 1616 GACGTC G[GA]^CG[CT]C
AflIII 757 ACATGT A^C[GA][CT]GT
AhdI 1616 GACGTCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1167 GTGCAC G^TGCAC
AocI 1405 CCTCAGG CC^T[ACGT]AGG
ApaLI 1167 GTGCAC G^TGCAC
Asp718I 144 GGTACC G^GTACC
AspEI 1616 GACGTCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1012 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 144 GGTACC G^G[CT][GA]CC
BanII 1245 GGGCTC G[GA]GC[CT]^C
BbrPI 951 CACGTG CAC^GTG
BbsI 262 GAAGAC GAAGAC(2/6)
BclI 179 TGATCA T^GATCA
BcnI 1940 CCCGG CC^[GC]GG
BpuAI 262 GAAGAC GAAGAC(2/6)
BsaAI 951 CACGTG [CT]AC^GT[GA]
BsaHI 1616 GACGTC G[GA]^CG[CT]C
BsmI 351 GCATTC GAATGC(1/-1)
BspDI 763 ATCGAT AT^CGAT
BspHI 6 TCATGA T^CATGA
BspLU11I 757 ACATGT A^CATGT
BsrDI 13 GCAATG GCAATG(2/0)
BsrFI 899 ACCGGC [GA]^CCGG[CT]
BssSI 1713 CACGAG CACGAG(-5/-1)
BstAPI 2167 GCAAATTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 1405 CCTCAGG CC^T[ACGT]AGG
CfoI 1068 GCGC GCG^C
Cfr10I 899 ACCGGC [GA]^CCGG[CT]
ClaI 763 ATCGAT AT^CGAT
DrdI 1012 GACATTGTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 1616 GACGTCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1405 CCTCAGG CC^T[ACGT]AGG
EcoNI 1083 CCTACTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 403 GAATTC G^AATTC
EcoRV 1389 GATATC GAT^ATC
EcoT22I 1483 ATGCAT ATGCA^T
FbaI 179 TGATCA T^GATCA
HhaI 1068 GCGC GCG^C
Hin1I 1616 GACGTC G[GA]^CG[CT]C
HinP1I 1068 GCGC G^CGC
KpnI 144 GGTACC GGTAC^C
NciI 1940 CCCGG CC^[GC]GG
NdeI 1808 CATATG CA^TATG
NsiI 1483 ATGCAT ATGCA^T
PflFI 1012 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 1139 GACTC GAGTC(4/5)
PmaCI 951 CACGTG CAC^GTG
PmlI 951 CACGTG CAC^GTG
PvuII 1796 CAGCTG CAG^CTG
RcaI 6 TCATGA T^CATGA
ScaI 1447 AGTACT AGT^ACT
SpeI 1054 ACTAGT A^CTAGT
SphI 395 GCATGC GCATG^C
TaqI 764 TCGA T^CGA
Tth111I 1012 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 764 TCGA T^CGA
XcmI 1751 CCAGATGCCCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AciI 220 CCGC CCGC(-3/-1)
374 GCGG
AlwNI 62 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
164 CAGCTCCTG
AspHI 524 GAGCAC G[AT]GC[AT]^C
1167 GTGCAC
BalI 77 TGGCCA TGG^CCA
1676 TGGCCA
BbvCI 1793 CCTCAGC CCTCAGC(-5/-2)
172 GCTGAGG
BsaBI 319 GATGGTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1969 GATAAAAATC
BseRI 103 CTCCTC GAGGAG(10/8)
1403 CTCCTC
BsgI 34 GTGCAG GTGCAG(16/14)
1726 GTGCAG
BsiHKAI 524 GAGCAC G[AT]GC[AT]^C
1167 GTGCAC
Bsp1407I 859 TGTACA T^GTACA
2058 TGTACA
BsrGI 859 TGTACA T^GTACA
2058 TGTACA
BstYI 313 GGATCT [GA]^GATC[CT]
1496 AGATCC
DsaI 1500 CCGTGG C^C[GA][CT]GG
1592 CCGTGG
EaeI 77 TGGCCA [CT]^GGCC[GA]
1676 TGGCCA
HincII 232 GTTGAC GT[CT]^[GA]AC
682 GTTGAC
HindII 232 GTTGAC GT[CT]^[GA]AC
682 GTTGAC
MamI 319 GATGGTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1969 GATAAAAATC
MflI 313 GGATCT [GA]^GATC[CT]
1496 AGATCC
MluNI 77 TGGCCA TGG^CCA
1676 TGGCCA
MscI 77 TGGCCA TGG^CCA
1676 TGGCCA
MslI 1476 CATTCCGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2139 CATCCTGGTG
MspA1I 372 CAGCGG C[AC]G^C[GT]G
1796 CAGCTG
PflMI 1423 CCAGGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1807 CCATATGGTGG
SapI 718 GAAGAGC GCTCTTC(1/4)
1461 GAAGAGC
SspBI 859 TGTACA T^GTACA
2058 TGTACA
StuI 1409 AGGCCT AGG^CCT
1963 AGGCCT
Van91I 1423 CCAGGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1807 CCATATGGTGG
XhoII 313 GGATCT [GA]^GATC[CT]
1496 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
AvaII BbvI BfaI BmyI BpmI
BsaI BsaJI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DpnII DraII EarI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MnlI MseI MspI MvaI MwoI
NdeII NlaIII NlaIV NspI PpuMI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI