Restriction map for
pFN21AE9439
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# NO CUT ENZYMES ( 126 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcsI AflII AgeI Alw44I AlwNI
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp718I AspI AviII AvrII
BamHI BanI BbeI BcgI BciVI
BclI BfaI BfrI BlnI BseAI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstZ17I CfoI Cfr10I
ClaI CpoI DraI DrdI EagI
EclXI Eco47III Eco52I EcoO65I EcoRI
EcoRV FbaI FseI FspI HaeII
HhaI HinP1I HincII HindII HindIII
HpaI KasI KpnI KspI MaeI
MfeI MluI MroI MseI MunI
MvnI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NspV
PacI PflFI PflMI PinAI PmeI
PpuMI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tru9I Tsp509I Tth111I Van91I
XbaI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AccI 1507 GTCTAC GT^[AC][GT]AC
AcyI 6 GACGCC G[GA]^CG[CT]C
AflIII 110 ACATGT A^C[GA][CT]GT
AhdI 51 GACCTCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 1365 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 51 GACCTCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 1475 TGGCCA TGG^CCA
BbrPI 925 CACGTG CAC^GTG
BbvCI 294 CCTCAGC CCTCAGC(-5/-2)
BglII 1367 AGATCT A^GATCT
BsaAI 925 CACGTG [CT]AC^GT[GA]
BsaBI 1176 GATGGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 6 GACGCC G[GA]^CG[CT]C
BsaWI 1415 ACCGGA [AT]^CCGG[AT]
BsgI 159 GTGCAG GTGCAG(16/14)
BsiEI 616 CGACCG CG[GA][CT]^CG
BspLU11I 110 ACATGT A^CATGT
BsrBI 1402 GAGCGG CCGCTC(-3/-3)
BsrDI 185 GCAATG GCAATG(2/0)
BstXI 11 CCAGGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraIII 153 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 51 GACCTCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 872 CCTTTCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 776 CCTTGG C^C[AT][AT]GG
EcoT22I 565 ATGCAT ATGCA^T
Hin1I 6 GACGCC G[GA]^CG[CT]C
MaeII 926 ACGT A^CGT
MamI 1176 GATGGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1475 TGGCCA TGG^CCA
MscI 1475 TGGCCA TGG^CCA
MslI 1059 CACCTGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NsiI 565 ATGCAT ATGCA^T
PaeR7I 1446 CTCGAG C^TCGAG
PmaCI 925 CACGTG CAC^GTG
PmlI 925 CACGTG CAC^GTG
PshAI 466 GACATTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 308 CTGCAG CTGCA^G
StyI 776 CCTTGG C^C[AT][AT]GG
TfiI 957 GAATC G^A[AT]TC
XhoI 1446 CTCGAG C^TCGAG
XmnI 1365 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AfaI 114 GTAC GT^AC
1284 GTAC
AlwI 416 GATCC GGATC(4/5)
921 GATCC
AocI 53 CCTCAGG CC^T[ACGT]AGG
715 CCTGAGG
AspHI 1426 GTGCTC G[AT]GC[AT]^C
1587 GAGCAC
BlpI 583 GCTGAGC GC^T[ACGT]AGC
1143 GCTGAGC
Bpu1102I 583 GCTGAGC GC^T[ACGT]AGC
1143 GCTGAGC
BsaI 768 GAGACC GGTCTC(1/5)
831 GGTCTC
BseRI 37 CTCCTC GAGGAG(10/8)
1599 CTCCTC
BsiHKAI 1426 GTGCTC G[AT]GC[AT]^C
1587 GAGCAC
BstYI 920 AGATCC [GA]^GATC[CT]
1367 AGATCT
Bsu36I 53 CCTCAGG CC^T[ACGT]AGG
715 CCTGAGG
CelII 583 GCTGAGC GC^T[ACGT]AGC
1143 GCTGAGC
EaeI 449 TGGCCG [CT]^GGCC[GA]
1475 TGGCCA
EarI 484 CTCTTC CTCTTC(1/4)
1602 CTCTTC
Eco81I 53 CCTCAGG CC^T[ACGT]AGG
715 CCTGAGG
HgaI 6 GACGC GACGC(5/10)
249 GACGC
Ksp632I 484 CTCTTC CTCTTC(1/4)
1602 CTCTTC
MflI 920 AGATCC [GA]^GATC[CT]
1367 AGATCT
MspA1I 519 CAGCGG C[AC]G^C[GT]G
1391 CAGCGG
NspI 110 ACATGT [GA]CATG^[CT]
567 GCATGT
PleI 178 GACTC GAGTC(4/5)
480 GACTC
RsaI 114 GTAC GT^AC
1284 GTAC
SfcI 308 CTGCAG C^T[GA][CT]AG
1388 CTACAG
SmaI 887 CCCGGG CCC^GGG
1019 CCCGGG
SmlI 1446 CTCGAG C^T[CT][GA]AG
1640 CTCAAG
TaqI 414 TCGA T^CGA
1447 TCGA
TthHB8I 414 TCGA T^CGA
1447 TCGA
XcmI 816 CCAGGCGGGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
836 CCATGATCTTCCTGG
XhoII 920 AGATCC [GA]^GATC[CT]
1367 AGATCT
XmaCI 887 CCCGGG C^CCGGG
1019 CCCGGG
XmaI 887 CCCGGG C^CCGGG
1019 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AciI AluI ApaI AvaI AvaII
BanII BbsI BbvI BcnI BglI
BmyI BpmI BpuAI BsaJI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
Cac8I Cfr13I DdeI DpnII DraII
DsaI EcoO109I EcoRII FauI Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI MaeIII MboI MboII
MnlI MspI MvaI MwoI NciI
NdeII NlaIII NlaIV Sau3AI Sau96I
ScrFI SfaNI TseI Tsp45I TspRI