Restriction map for
pFN21AE9431
##################################
# NO CUT ENZYMES ( 102 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AocI ApoI
AscI AseI AsnI Asp700I Asp718I
AspI AvrII BamHI BbrPI BciVI
BclI BfrI BlnI BsaAI BsaBI
BsaWI BseAI BsiWI BsmBI BsmI
Bsp1407I BspEI BspHI BspLU11I BsrGI
BsrI Bst1107I BstBI BstEII BstPI
BstZ17I Bsu36I CpoI DraI DrdI
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI HindIII HpaI
KpnI MamI MfeI MluI MroI
MseI MunI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SalI SapI ScaI
SfuI SgrAI SmlI SnaBI SpeI
SphI SspBI SspI StuI SwaI
TfiI Tru9I Tsp509I Tth111I XbaI
XhoI XmnI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AfaI 140 GTAC GT^AC
AflIII 1202 ACGTGT A^C[GA][CT]GT
AhdI 961 GACGAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 382 GTGCAC G^TGCAC
AlwI 1597 GATCC GGATC(4/5)
ApaLI 382 GTGCAC G^TGCAC
AspEI 961 GACGAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 1659 TGCGCA TGC^GCA
BfaI 1063 CTAG C^TAG
BglII 1085 AGATCT A^GATCT
BlpI 357 GCTGAGC GC^T[ACGT]AGC
BpmI 1096 CTGGAG CTGGAG(16/14)
Bpu1102I 357 GCTGAGC GC^T[ACGT]AGC
BspDI 688 ATCGAT AT^CGAT
BsrDI 362 GCAATG GCAATG(2/0)
BssSI 733 CTCGTG CACGAG(-5/-1)
CelII 357 GCTGAGC GC^T[ACGT]AGC
ClaI 688 ATCGAT AT^CGAT
DraIII 1276 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 1367 CGGCCG C^GGCCG
Eam1105I 961 GACGAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 583 CTCTTC CTCTTC(1/4)
EclXI 1367 CGGCCG C^GGCCG
Eco52I 1367 CGGCCG C^GGCCG
FspI 1659 TGCGCA TGC^GCA
HincII 346 GTCAAC GT[CT]^[GA]AC
HindII 346 GTCAAC GT[CT]^[GA]AC
Ksp632I 583 CTCTTC CTCTTC(1/4)
KspI 1414 CCGCGG CCGC^GG
MaeI 1063 CTAG C^TAG
MaeII 1202 ACGT A^CGT
NaeI 1672 GCCGGC GCC^GGC
NgoMIV 1672 GCCGGC G^CCGGC
PvuII 202 CAGCTG CAG^CTG
RsaI 140 GTAC GT^AC
SacII 1414 CCGCGG CCGC^GG
SbfI 1158 CCTGCAGG CCTGCA^GG
SfiI 945 GGCCCTCATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Sse8387I 1158 CCTGCAGG CCTGCA^GG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
Aor51HI 461 AGCGCT AGC^GCT
1022 AGCGCT
AspHI 382 GTGCAC G[AT]GC[AT]^C
852 GTGCTC
BbsI 253 GTCTTC GAAGAC(2/6)
748 GTCTTC
BbvCI 87 CCTCAGC CCTCAGC(-5/-2)
818 CCTCAGC
BcgI 248 GCAAGGTCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
569 GCACAGCCATCG
BpuAI 253 GTCTTC GAAGAC(2/6)
748 GTCTTC
BsaI 979 GAGACC GGTCTC(1/5)
241 GGTCTC
BseRI 681 CTCCTC GAGGAG(10/8)
964 GAGGAG
BsiEI 900 CGACCG CG[GA][CT]^CG
1367 CGGCCG
BsiHKAI 382 GTGCAC G[AT]GC[AT]^C
852 GTGCTC
BsmAI 979 GAGAC GTCTC(1/5)
242 GTCTC
BsrFI 353 ACCGGC [GA]^CCGG[CT]
1672 GCCGGC
BstYI 1085 AGATCT [GA]^GATC[CT]
1596 AGATCC
Cfr10I 353 ACCGGC [GA]^CCGG[CT]
1672 GCCGGC
Eco47III 461 AGCGCT AGC^GCT
1022 AGCGCT
MaeIII 301 GTCAC ^GT[ACGT]AC
736 GTGAC
MflI 1085 AGATCT [GA]^GATC[CT]
1596 AGATCC
NcoI 474 CCATGG C^CATGG
554 CCATGG
NspI 158 ACATGC [GA]CATG^[CT]
323 GCATGT
PflMI 71 CCAATGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
588 CCAGACGCTGG
PpuMI 1163 AGGTCCC [GA]G^G[AT]CC[CT]
1256 AGGACCT
PstI 718 CTGCAG CTGCA^G
1159 CTGCAG
SexAI 1010 ACCTGGT A^CC[AT]GGT
1277 ACCTGGT
SfcI 718 CTGCAG C^T[GA][CT]AG
1159 CTGCAG
Tsp45I 301 GTCAC ^GT[GC]AC
736 GTGAC
Van91I 71 CCAATGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
588 CCAGACGCTGG
XcmI 185 CCACCTACTCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
644 CCACCATCGTGGTGG
XhoII 1085 AGATCT [GA]^GATC[CT]
1596 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 89 enzymes) #
#########################################
AccII AciI AcyI AluI AlwNI
ApaI AvaI AvaII BalI BanI
BanII BbeI BbvI BcnI BglI
BmyI BsaHI BsaJI BsgI BsiYI
BslI BsmFI BsoBI Bsp1286I BspMI
BsrBI BssHII BssKI BstAPI BstF5I
BstNI BstUI BstXI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KasI MboI
MboII MluNI MnlI MscI MslI
MspA1I MspI MvaI MvnI MwoI
NarI NciI NdeII NlaIII NlaIV
PleI Sau3AI Sau96I ScrFI SfaNI
SfoI SmaI StyI TaqI TseI
TspRI TthHB8I XmaCI XmaI