Restriction map for
pFN21AE9425
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# NO CUT ENZYMES ( 103 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI Alw44I Aor51HI ApaLI AscI
AseI AsnI Asp718I AspI AvaI
AvrII BanI BbeI BbrPI BciVI
BclI BcnI BglI BlnI BlpI
Bpu1102I BsaAI BsaHI BsaI BseAI
BsiEI BsiWI BsmBI BsoBI BspDI
BspEI BspHI BssHII BssSI Bst1107I
BstBI BstUI BstZ17I CelII ClaI
CpoI EagI EclXI Eco47III Eco52I
EcoNI FbaI FseI HaeII Hin1I
HpaI KasI KpnI KspI MluI
MroI MvnI NaeI NarI NciI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflFI PflMI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI RcaI RsrII SacII
SbfI ScaI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SwaI Tth111I Van91I XbaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccI 1000 GTCGAC GT^[AC][GT]AC
AgeI 223 ACCGGT A^CCGGT
AhdI 1125 GACCCTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 2254 CAGAATCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1189 CCTGAGG CC^T[ACGT]AGG
ApaI 1352 GGGCCC GGGCC^C
AspEI 1125 GACCCTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 3116 TGCGCA TGC^GCA
BcgI 371 CGAATGAGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 3053 AGATCT A^GATCT
BpmI 1292 CTGGAG CTGGAG(16/14)
BsaBI 3535 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 2653 CTCCTC GAGGAG(10/8)
Bsp1407I 2780 TGTACA T^GTACA
BspMI 3113 ACCTGC ACCTGC(4/8)
BsrBI 1084 CCGCTC CCGCTC(-3/-3)
BsrFI 223 ACCGGT [GA]^CCGG[CT]
BsrGI 2780 TGTACA T^GTACA
BstAPI 3166 GCATTCTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1620 GGTAACC G^GT[ACGT]ACC
BstPI 1620 GGTAACC G^GT[ACGT]ACC
Bsu36I 1189 CCTGAGG CC^T[ACGT]AGG
CfoI 3117 GCGC GCG^C
Cfr10I 223 ACCGGT [GA]^CCGG[CT]
DraIII 97 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1475 GACAACTGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 1125 GACCCTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1189 CCTGAGG CC^T[ACGT]AGG
EcoO65I 1620 GGTAACC G^GT[ACGT]ACC
EcoRI 2947 GAATTC G^AATTC
EcoRV 1642 GATATC GAT^ATC
FauI 1083 CCCGC CCCGC(4/6)
FspI 3116 TGCGCA TGC^GCA
HgaI 207 GACGC GACGC(5/10)
HhaI 3117 GCGC GCG^C
HinP1I 3117 GCGC G^CGC
HincII 1000 GTCGAC GT[CT]^[GA]AC
HindII 1000 GTCGAC GT[CT]^[GA]AC
MamI 3535 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NdeI 2570 CATATG CA^TATG
PinAI 223 ACCGGT A^CCGGT
SacI 535 GAGCTC GAGCT^C
SalI 1000 GTCGAC G^TCGAC
SapI 2141 GAAGAGC GCTCTTC(1/4)
SexAI 500 ACCAGGT A^CC[AT]GGT
SfcI 1149 CTATAG C^T[GA][CT]AG
SspBI 2780 TGTACA T^GTACA
StuI 348 AGGCCT AGG^CCT
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# TWO CUT ENZYMES ( 17 enzymes) #
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AflII 456 CTTAAG C^TTAAG
1830 CTTAAG
BamHI 2067 GGATCC G^GATCC
2265 GGATCC
BbvCI 972 CCTCAGC CCTCAGC(-5/-2)
1721 CCTCAGC
BfrI 456 CTTAAG C^TTAAG
1830 CTTAAG
BsaWI 223 ACCGGT [AT]^CCGG[AT]
3083 ACCGGA
BsmI 951 GAATGC GAATGC(1/-1)
3166 GCATTC
BspLU11I 1926 ACATGT A^CATGT
2777 ACATGT
DsaI 1496 CCATGG C^C[GA][CT]GG
3500 CCATGG
HapII 224 CCGG C^CGG
3084 CCGG
HindIII 744 AAGCTT A^AGCTT
1871 AAGCTT
HpaII 224 CCGG C^CGG
3084 CCGG
MaeII 1547 ACGT A^CGT
2371 ACGT
MspA1I 975 CAGCTG C[AC]G^C[GT]G
1107 CAGCTG
MspI 224 CCGG C^CGG
3084 CCGG
NcoI 1496 CCATGG C^CATGG
3500 CCATGG
PvuII 975 CAGCTG CAG^CTG
1107 CAGCTG
XcmI 116 CCAACTGCCGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3029 CCATGTTCATTGTGG
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# 3 OR MORE CUT ENZYMES ( 90 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI ApoI Asp700I AspHI AvaII
BalI BanII BbsI BbvI BfaI
BmyI BpuAI BsaJI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrDI BsrI BssKI BstF5I BstNI
BstXI BstYI Cac8I Cfr13I DdeI
DpnII DraI DraII EaeI EarI
EcoO109I EcoRII EcoT14I EcoT22I Fnu4HI
FokI HaeIII HinfI HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MfeI MflI MluNI MnlI MscI
MseI MslI MunI MvaI MwoI
NdeII NlaIII NlaIV NsiI NspI
PleI PpuMI RsaI Sau3AI Sau96I
ScrFI SfaNI SmlI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII XmnI