Restriction map for
pFN21AE4219
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# NO CUT ENZYMES ( 102 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AflIII AgeI Alw44I AocI Aor51HI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspI AviII AvrII BamHI
BbrPI BbvCI BcgI BfrI BglII
BlnI BsaAI BsaBI BsaWI BseAI
BsiWI Bsp1407I BspDI BspEI BspLU11I
BsrGI BssHII Bst1107I BstBI BstEII
BstPI BstZ17I Bsu36I ClaI CpoI
DraI DraIII DrdI EagI EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRI
EcoRV FseI FspI HpaI KpnI
MamI MfeI MluI MroI MunI
NcoI NdeI NheI NotI NruI
NspV PacI PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RsrII SalI SapI
SbfI ScaI SexAI SfiI SfuI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Tth111I Van91I
XbaI XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AccI 97 GTAGAC GT^[AC][GT]AC
AcsI 1462 AAATTC [GA]^AATT[CT]
AhdI 499 GACCCCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 1462 AAATTC [GA]^AATT[CT]
AspEI 499 GACCCCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbsI 1840 GAAGAC GAAGAC(2/6)
BciVI 1952 GGATAC GTATCC(6/5)
BclI 1212 TGATCA T^GATCA
BpuAI 1840 GAAGAC GAAGAC(2/6)
BsaI 848 GGTCTC GGTCTC(1/5)
BsgI 1591 CTGCAC GTGCAG(16/14)
BsiEI 564 CGACCG CG[GA][CT]^CG
BsmBI 418 GAGACG CGTCTC(1/5)
BspHI 876 TCATGA T^CATGA
BspMI 1227 ACCTGC ACCTGC(4/8)
BstAPI 315 GCAGCTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 1800 CCAGCGGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Eam1105I 499 GACCCCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 1678 CCTGTCAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 1632 ATGCAT ATGCA^T
FbaI 1212 TGATCA T^GATCA
HindIII 1495 AAGCTT A^AGCTT
HphI 863 TCACC GGTGA(8/7)
KspI 431 CCGCGG CCGC^GG
NaeI 366 GCCGGC GCC^GGC
NgoMIV 366 GCCGGC G^CCGGC
NsiI 1632 ATGCAT ATGCA^T
PaeR7I 259 CTCGAG C^TCGAG
PstI 125 CTGCAG CTGCA^G
RcaI 876 TCATGA T^CATGA
SacII 431 CCGCGG CCGC^GG
SfcI 125 CTGCAG C^T[GA][CT]AG
SgrAI 365 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 236 CCCGGG CCC^GGG
Tsp45I 1309 GTCAC ^GT[GC]AC
XcmI 2017 CCAGCCTGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 259 CTCGAG C^TCGAG
XmaCI 236 CCCGGG C^CCGGG
XmaI 236 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AlwNI 22 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
289 CAGCAGCTG
ApaI 105 GGGCCC GGGCC^C
132 GGGCCC
AvaI 236 CCCGGG C^[CT]CG[GA]G
259 CTCGAG
BalI 519 TGGCCA TGG^CCA
611 TGGCCA
BbeI 363 GGCGCC GGCGC^C
369 GGCGCC
BpmI 310 CTGGAG CTGGAG(16/14)
943 CTGGAG
BsmI 882 GAATGC GAATGC(1/-1)
1273 GAATGC
BsoBI 236 CCCGGG C^[CT]CG[GA]G
259 CTCGAG
BsrBI 1746 CCGCTC CCGCTC(-3/-3)
283 GAGCGG
BsrDI 1144 CATTGC GCAATG(2/0)
2083 GCAATG
BsrFI 366 GCCGGC [GA]^CCGG[CT]
566 ACCGGC
BssSI 742 CACGAG CACGAG(-5/-1)
1647 CACGAG
BstYI 1200 AGATCC [GA]^GATC[CT]
1674 AGATCC
Cfr10I 366 GCCGGC [GA]^CCGG[CT]
566 ACCGGC
DsaI 404 CCGTGG C^C[GA][CT]GG
431 CCGCGG
HincII 673 GTCAAC GT[CT]^[GA]AC
1756 GTTGAC
HindII 673 GTCAAC GT[CT]^[GA]AC
1756 GTTGAC
KasI 363 GGCGCC G^GCGCC
369 GGCGCC
MaeII 549 ACGT A^CGT
677 ACGT
MaeIII 1309 GTCAC ^GT[ACGT]AC
1610 GTTAC
MflI 1200 AGATCC [GA]^GATC[CT]
1674 AGATCC
MluNI 519 TGGCCA TGG^CCA
611 TGGCCA
MscI 519 TGGCCA TGG^CCA
611 TGGCCA
NarI 363 GGCGCC GG^CGCC
369 GGCGCC
NspI 907 GCATGT [GA]CATG^[CT]
1661 GCATGT
PpuMI 181 GGGACCT [GA]G^G[AT]CC[CT]
2089 AGGTCCT
SfoI 363 GGCGCC GGC^GCC
369 GGCGCC
SmlI 259 CTCGAG C^T[CT][GA]AG
790 CTCAAG
XhoII 1200 AGATCC [GA]^GATC[CT]
1674 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AspHI AvaII BanI BanII
BbvI BcnI BfaI BglI BlpI
BmyI Bpu1102I BsaHI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
BstUI Cac8I CelII CfoI Cfr13I
DdeI DpnII DraII EaeI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
ItaI Ksp632I MaeI MboI MboII
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PleI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
StyI TaqI TfiI Tru9I TseI
Tsp509I TspRI TthHB8I