Restriction map for
pFN21AE3168
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# NO CUT ENZYMES ( 156 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcsI AfaI AflII AflIII AgeI
AhdI Alw44I AlwNI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BalI
BanI BbeI BbrPI BbvCI BcgI
BclI BfaI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaI BsaWI BseAI BseRI BsgI
BsiWI BsmFI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BsrGI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstZ17I
CelII ClaI CpoI DraI DraIII
DrdI DsaI Eam1105I EcoNI EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FbaI
FseI FspI HincII HindII HindIII
HpaI KasI KpnI KspI MaeI
MaeIII MamI MfeI MluI MluNI
MroI MscI MseI MunI MvnI
NarI NcoI NdeI NheI NlaIII
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsaI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfcI SfiI SfoI
SfuI SgrAI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
StyI SwaI TfiI Tru9I Tsp45I
Tsp509I Tth111I XbaI XcmI XhoI
XmnI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AccI 445 GTCTAC GT^[AC][GT]AC
AocI 88 CCTGAGG CC^T[ACGT]AGG
Aor51HI 410 AGCGCT AGC^GCT
BamHI 78 GGATCC G^GATCC
BciVI 192 GGATAC GTATCC(6/5)
BpmI 466 CTGGAG CTGGAG(16/14)
BsiEI 24 CGGCCG CG[GA][CT]^CG
BsmAI 254 GTCTC GTCTC(1/5)
BsmBI 253 CGTCTC CGTCTC(1/5)
BssSI 259 CACGAG CACGAG(-5/-1)
BstXI 143 CCATCAACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 88 CCTGAGG CC^T[ACGT]AGG
DdeI 89 CTGAG C^T[ACGT]AG
EagI 24 CGGCCG C^GGCCG
EarI 334 GAAGAG CTCTTC(1/4)
EclXI 24 CGGCCG C^GGCCG
Eco47III 410 AGCGCT AGC^GCT
Eco52I 24 CGGCCG C^GGCCG
Eco81I 88 CCTGAGG CC^T[ACGT]AGG
HaeII 410 AGCGCT [GA]GCGC^[CT]
HinfI 337 GAGTC G^A[ACGT]TC
HphI 63 GGTGA GGTGA(8/7)
Ksp632I 334 GAAGAG CTCTTC(1/4)
MslI 259 CACGAGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PflMI 549 CCAGTGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 337 GAGTC GAGTC(4/5)
PpuMI 119 AGGACCT [GA]G^G[AT]CC[CT]
SacI 211 GAGCTC GAGCT^C
SfaNI 29 GCATC GCATC(5/9)
SmaI 276 CCCGGG CCC^GGG
TaqI 340 TCGA T^CGA
TthHB8I 340 TCGA T^CGA
Van91I 549 CCAGTGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 276 CCCGGG C^CCGGG
XmaI 276 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AcyI 370 GACGCC G[GA]^CG[CT]C
424 GGCGTC
AluI 166 AGCT AG^CT
212 AGCT
AspHI 211 GAGCTC G[AT]GC[AT]^C
469 GAGCAC
AvaI 276 CCCGGG C^[CT]CG[GA]G
403 CCCGAG
AvaII 120 GGACC G^G[AT]CC
503 GGACC
BbsI 480 GAAGAC GAAGAC(2/6)
10 GTCTTC
BpuAI 480 GAAGAC GAAGAC(2/6)
10 GTCTTC
BsaHI 370 GACGCC G[GA]^CG[CT]C
424 GGCGTC
BsiHKAI 211 GAGCTC G[AT]GC[AT]^C
469 GAGCAC
BsoBI 276 CCCGGG C^[CT]CG[GA]G
403 CCCGAG
BsrFI 22 GCCGGC [GA]^CCGG[CT]
356 GCCGGC
BsrI 189 ACTGG ACTGG(1/-1)
549 CCAGT
BstF5I 390 CATCC GGATG(2/0)
264 GGATG
BstYI 78 GGATCC [GA]^GATC[CT]
432 GGATCT
Cfr10I 22 GCCGGC [GA]^CCGG[CT]
356 GCCGGC
DpnII 79 GATC ^GATC
433 GATC
EaeI 24 CGGCCG [CT]^GGCC[GA]
302 CGGCCA
FauI 19 CCCGC CCCGC(4/6)
82 CCCGC
FokI 390 CATCC GGATG(9/13)
264 GGATG
Hin1I 370 GACGCC G[GA]^CG[CT]C
424 GGCGTC
MaeII 218 ACGT A^CGT
252 ACGT
MboI 79 GATC ^GATC
433 GATC
MflI 78 GGATCC [GA]^GATC[CT]
432 GGATCT
MspA1I 69 CCGCTG C[AC]G^C[GT]G
160 CAGCGG
NaeI 22 GCCGGC GCC^GGC
356 GCCGGC
NdeII 79 GATC ^GATC
433 GATC
NgoMIV 22 GCCGGC G^CCGGC
356 GCCGGC
Sau3AI 79 GATC ^GATC
433 GATC
XhoII 78 GGATCC [GA]^GATC[CT]
432 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 38 enzymes) #
#########################################
AciI AlwI ApaI BanII BbvI
BcnI BmyI BsaJI BsiYI BslI
Bsp1286I BssKI BstNI Cac8I CfoI
Cfr13I DraII EcoO109I EcoRII Fnu4HI
HaeIII HapII HgaI HhaI HinP1I
HpaII ItaI MboII MnlI MspI
MvaI MwoI NciI NlaIV Sau96I
ScrFI TseI TspRI