Restriction map for
pFN21AB7933
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# NO CUT ENZYMES ( 120 enzymes) #
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AatII AccI AclI AcsI AflII
AgeI Alw44I AlwNI AocI Aor51HI
ApaLI ApoI AscI AseI AsnI
AspI AviII AvrII BbeI BbrPI
BbvCI BcgI BclI BfaI BfrI
BlnI BsaAI BsaBI BsiWI BsmBI
Bsp1407I BspDI BspHI BspLU11I BspMI
BsrBI BsrGI BsrI BssHII Bst1107I
BstAPI BstEII BstPI BstZ17I Bsu36I
ClaI CpoI DraI DraIII DrdI
Eco47III Eco81I EcoO65I EcoRI EcoT14I
EcoT22I FbaI FseI FspI HincII
HindII HindIII HpaI HphI KasI
MaeI MaeII MaeIII MamI MfeI
MseI MunI NarI NcoI NdeI
NheI NruI NsiI NspI PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI RcaI RsrII SalI
SapI SbfI ScaI SexAI SfcI
SfoI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI StyI
SwaI TfiI Tru9I Tsp45I Tsp509I
TspRI Tth111I XbaI XcmI XhoI
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# ONE CUT ENZYMES ( 49 enzymes) #
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Acc65I 566 GGTACC G^GTACC
AccIII 730 TCCGGA T^CCGGA
AcyI 600 GACGCC G[GA]^CG[CT]C
AflIII 1076 ACGCGT A^C[GA][CT]GT
AhdI 825 GACAGAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 787 GGGCCC GGGCC^C
Asp700I 756 GAAGACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 566 GGTACC G^GTACC
AspEI 825 GACAGAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 1092 GAGCTC G[AT]GC[AT]^C
AvaII 863 GGACC G^G[AT]CC
BalI 896 TGGCCA TGG^CCA
BciVI 117 GTATCC GTATCC(6/5)
BglII 740 AGATCT A^GATCT
BsaHI 600 GACGCC G[GA]^CG[CT]C
BsaI 717 GAGACC GGTCTC(1/5)
BseAI 730 TCCGGA T^CCGGA
BsiHKAI 1092 GAGCTC G[AT]GC[AT]^C
BsmFI 644 GGGAC GGGAC(10/14)
BsmI 40 GCATTC GAATGC(1/-1)
BspEI 730 TCCGGA T^CCGGA
BsrDI 16 GCAATG GCAATG(2/0)
BssSI 714 CACGAG CACGAG(-5/-1)
BstBI 763 TTCGAA TT^CGAA
Eam1105I 825 GACAGAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 667 CCTCATCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 298 GATATC GAT^ATC
Hin1I 600 GACGCC G[GA]^CG[CT]C
KpnI 566 GGTACC GGTAC^C
MluI 1076 ACGCGT A^CGCGT
MluNI 896 TGGCCA TGG^CCA
MroI 730 TCCGGA T^CCGGA
MscI 896 TGGCCA TGG^CCA
MslI 318 CATGCCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NotI 433 GCGGCCGC GC^GGCCGC
NspV 763 TTCGAA TT^CGAA
PflMI 605 CCATCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 1021 CAGCTG CAG^CTG
SacI 1092 GAGCTC GAGCT^C
SfiI 327 GGCCTCGGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 763 TTCGAA TT^CGAA
SmaI 517 CCCGGG CCC^GGG
SmlI 937 CTCAAG C^T[CT][GA]AG
TaqI 764 TCGA T^CGA
TthHB8I 764 TCGA T^CGA
Van91I 605 CCATCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 517 CCCGGG C^CCGGG
XmaI 517 CCCGGG C^CCGGG
XmnI 756 GAAGACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 19 enzymes) #
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BamHI 676 GGATCC G^GATCC
990 GGATCC
BbsI 93 GAAGAC GAAGAC(2/6)
756 GAAGAC
BlpI 48 GCTCAGC GC^T[ACGT]AGC
583 GCTGAGC
BpmI 639 CTGGAG CTGGAG(16/14)
751 CTGGAG
Bpu1102I 48 GCTCAGC GC^T[ACGT]AGC
583 GCTGAGC
BpuAI 93 GAAGAC GAAGAC(2/6)
756 GAAGAC
BsaWI 361 TCCGGT [AT]^CCGG[AT]
730 TCCGGA
BsgI 73 CTGCAC GTGCAG(16/14)
1072 CTGCAC
BsmAI 717 GAGAC GTCTC(1/5)
925 GAGAC
CelII 48 GCTCAGC GC^T[ACGT]AGC
583 GCTGAGC
DraII 786 GGGGCCC [GA]G^G[ACGT]CC[CT]
943 GGGGCCT
EcoO109I 786 GGGGCCC [GA]G^G[ACGT]CC[CT]
943 GGGGCCT
HaeII 538 AGCGCC [GA]GCGC^[CT]
932 AGCGCC
HinfI 831 GAGTC G^A[ACGT]TC
919 GACTC
NaeI 133 GCCGGC GCC^GGC
308 GCCGGC
NgoMIV 133 GCCGGC G^CCGGC
308 GCCGGC
NlaIII 81 CATG CATG^
318 CATG
PleI 919 GACTC GAGTC(4/5)
831 GAGTC
SfaNI 624 GATGC GCATC(5/9)
290 GCATC
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# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AvaI BanI BanII BbvI BcnI
BglI BmyI BsaJI BseRI BsiEI
BsiYI BslI BsoBI Bsp1286I BsrFI
BssKI BstF5I BstNI BstUI BstXI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DsaI EaeI EagI
EarI EclXI Eco52I EcoRII FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HpaII ItaI Ksp632I
KspI MboI MboII MflI MnlI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIV RsaI SacII
Sau3AI Sau96I ScrFI TseI XhoII