Restriction map for
pFN21AE0949
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# NO CUT ENZYMES ( 132 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AcyI AflIII AgeI AhdI Alw44I
AlwI AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp718I
AspEI AspI AvrII BamHI BanI
BbeI BbrPI BbsI BcgI BclI
BlnI BpuAI BsaBI BsaHI BsaWI
BseAI BsgI BsiEI BsiWI BsmBI
BsmI BspDI BspEI BspLU11I BspMI
BsrBI BsrDI BsrFI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstZ17I
Bsu36I Cfr10I ClaI CpoI DraI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI HaeII
HgaI Hin1I HincII HindII HpaI
KasI KpnI KspI MamI MfeI
MluI MroI MunI NaeI NarI
NdeI NgoMIV NheI NlaIV NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI PvuII
RsrII SacII SalI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI SspI StuI
SwaI Tth111I XbaI XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AccI 625 GTCTAC GT^[AC][GT]AC
AccII 944 CGCG CG^CG
AflII 69 CTTAAG C^TTAAG
AlwNI 856 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 1174 GAATTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 135 GAGCTC G[AT]GC[AT]^C
AvaI 736 CCCGAG C^[CT]CG[GA]G
AviII 641 TGCGCA TGC^GCA
BciVI 900 GGATAC GTATCC(6/5)
BfrI 69 CTTAAG C^TTAAG
BglI 1156 GCCTCAGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 468 AGATCT A^GATCT
BlpI 729 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 729 GCTCAGC GC^T[ACGT]AGC
BsaAI 286 TACGTG [CT]AC^GT[GA]
BsaI 42 GGTCTC GGTCTC(1/5)
BseRI 922 GAGGAG GAGGAG(10/8)
BsiHKAI 135 GAGCTC G[AT]GC[AT]^C
BsmFI 829 GTCCC GGGAC(10/14)
BsoBI 736 CCCGAG C^[CT]CG[GA]G
BspHI 173 TCATGA T^CATGA
BstAPI 528 GCAGGGGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 944 CGCG CG^CG
BstXI 323 CCAATGAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 468 AGATCT [GA]^GATC[CT]
CelII 729 GCTCAGC GC^T[ACGT]AGC
DraIII 1060 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoT14I 666 CCATGG C^C[AT][AT]GG
FauI 916 CCCGC CCCGC(4/6)
FspI 641 TGCGCA TGC^GCA
HindIII 314 AAGCTT A^AGCTT
MflI 468 AGATCT [GA]^GATC[CT]
MvnI 944 CGCG CG^CG
NcoI 666 CCATGG C^CATGG
NspI 357 GCATGT [GA]CATG^[CT]
PstI 526 CTGCAG CTGCA^G
RcaI 173 TCATGA T^CATGA
SacI 135 GAGCTC GAGCT^C
SapI 1264 GAAGAGC GCTCTTC(1/4)
SbfI 525 CCTGCAGG CCTGCA^GG
SmlI 69 CTTAAG C^T[CT][GA]AG
Sse8387I 525 CCTGCAGG CCTGCA^GG
StyI 666 CCATGG C^C[AT][AT]GG
XhoII 468 AGATCT [GA]^GATC[CT]
XmnI 1174 GAATTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
BalI 815 TGGCCA TGG^CCA
953 TGGCCA
BanII 135 GAGCTC G[GA]GC[CT]^C
936 GAGCCC
BbvCI 651 CCTCAGC CCTCAGC(-5/-2)
919 GCTGAGG
Bsp1407I 478 TGTACA T^GTACA
573 TGTACA
BsrGI 478 TGTACA T^GTACA
573 TGTACA
DsaI 666 CCATGG C^C[GA][CT]GG
724 CCGTGG
EarI 370 CTCTTC CTCTTC(1/4)
1264 GAAGAG
Ksp632I 370 CTCTTC CTCTTC(1/4)
1264 GAAGAG
MaeII 287 ACGT A^CGT
1152 ACGT
MluNI 815 TGGCCA TGG^CCA
953 TGGCCA
MscI 815 TGGCCA TGG^CCA
953 TGGCCA
MslI 577 CACAGGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
852 CACCCAGATG
MspA1I 673 CAGCGG C[AC]G^C[GT]G
917 CCGCTG
PflMI 558 CCACCACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1059 CCACTTTGTGG
PleI 1012 GACTC GAGTC(4/5)
142 GAGTC
PpuMI 239 AGGACCT [GA]G^G[AT]CC[CT]
740 AGGTCCT
SfcI 526 CTGCAG C^T[GA][CT]AG
786 CTACAG
SspBI 478 TGTACA T^GTACA
573 TGTACA
TaqI 399 TCGA T^CGA
548 TCGA
Tsp509I 1175 AATT ^AATT
1204 AATT
TthHB8I 399 TCGA T^CGA
548 TCGA
Van91I 558 CCACCACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1059 CCACTTTGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AciI AfaI AluI AvaII BbvI
BcnI BfaI BmyI BpmI BsaJI
BsiYI BslI BsmAI Bsp1286I BsrI
BssKI BstF5I BstNI Cac8I CfoI
Cfr13I DdeI DpnII DraII EaeI
EcoO109I EcoRII Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI MaeI MaeIII MboI
MboII MnlI MseI MspI MvaI
MwoI NciI NdeII NlaIII RsaI
Sau3AI Sau96I ScrFI SfaNI TfiI
Tru9I TseI Tsp45I TspRI