Restriction map for
pFN21AE3169
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# NO CUT ENZYMES ( 122 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AfaI AflII AgeI AhdI
Alw44I AocI ApaLI ApoI AscI
AseI AsnI Asp718I AspEI AspHI
AspI AvaI AvrII BamHI BbrPI
BciVI BfrI BglII BlnI BlpI
Bpu1102I BsaWI BseAI BsiHKAI BsiWI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrGI BssSI
Bst1107I BstAPI BstEII BstPI BstXI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DraIII DrdI Eam1105I Eco81I
EcoO65I EcoRI EcoRV EcoT22I FseI
HindIII HpaI KpnI MfeI MluI
MroI MseI MslI MunI NdeI
NheI NotI NruI NsiI NspI
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsaI
RsrII SacI SalI SapI SbfI
ScaI SexAI SfiI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI TfiI Tru9I
Tsp509I Tth111I Van91I XbaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AflIII 461 ACGTGT A^C[GA][CT]GT
Asp700I 240 GAACGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 44 TGCGCA TGC^GCA
BalI 851 TGGCCA TGG^CCA
BbsI 472 GAAGAC GAAGAC(2/6)
BbvCI 870 CCTCAGC CCTCAGC(-5/-2)
BcgI 126 GCAGAGCCGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1064 TGATCA T^GATCA
BfaI 722 CTAG C^TAG
BpmI 525 CTCCAG CTGGAG(16/14)
BpuAI 472 GAAGAC GAAGAC(2/6)
BsaAI 460 TACGTG [CT]AC^GT[GA]
BsaI 340 GAGACC GGTCTC(1/5)
BsiEI 1179 CGGCCG CG[GA][CT]^CG
BspMI 385 GCAGGT ACCTGC(4/8)
BsrDI 1068 CATTGC GCAATG(2/0)
BstBI 64 TTCGAA TT^CGAA
BstYI 77 AGATCC [GA]^GATC[CT]
DraII 866 AGGCCCT [GA]G^G[ACGT]CC[CT]
EagI 1179 CGGCCG C^GGCCG
EclXI 1179 CGGCCG C^GGCCG
Eco52I 1179 CGGCCG C^GGCCG
EcoNI 837 CCTTCGACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 866 AGGCCCT [GA]G^G[ACGT]CC[CT]
FbaI 1064 TGATCA T^GATCA
FspI 44 TGCGCA TGC^GCA
HgaI 319 GACGC GACGC(5/10)
HincII 907 GTTGAC GT[CT]^[GA]AC
HindII 907 GTTGAC GT[CT]^[GA]AC
MaeI 722 CTAG C^TAG
MaeIII 603 GTCAC ^GT[ACGT]AC
MflI 77 AGATCC [GA]^GATC[CT]
MluNI 851 TGGCCA TGG^CCA
MscI 851 TGGCCA TGG^CCA
NaeI 829 GCCGGC GCC^GGC
NcoI 541 CCATGG C^CATGG
NgoMIV 829 GCCGGC G^CCGGC
NlaIII 542 CATG CATG^
NspV 64 TTCGAA TT^CGAA
PstI 622 CTGCAG CTGCA^G
SfaNI 173 GCATC GCATC(5/9)
SfcI 622 CTGCAG C^T[GA][CT]AG
SfuI 64 TTCGAA TT^CGAA
StuI 961 AGGCCT AGG^CCT
Tsp45I 603 GTCAC ^GT[GC]AC
XcmI 815 CCATCCGGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 77 AGATCC [GA]^GATC[CT]
XmnI 240 GAACGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AcyI 298 GGCGCC G[GA]^CG[CT]C
375 GGCGCC
AlwI 78 GATCC GGATC(4/5)
84 GATCC
AvaII 486 GGACC G^G[AT]CC
630 GGTCC
BbeI 298 GGCGCC GGCGC^C
375 GGCGCC
BglI 4 GCCTCGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
700 GCCCAAGTGGC
BsaBI 78 GATCCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1184 GATGGAAATC
BsaHI 298 GGCGCC G[GA]^CG[CT]C
375 GGCGCC
BsmFI 167 GGGAC GGGAC(10/14)
846 GGGAC
BsrBI 1114 CCGCTC CCGCTC(-3/-3)
151 GAGCGG
BssHII 96 GCGCGC G^CGCGC
122 GCGCGC
BstF5I 354 CATCC GGATG(2/0)
816 CATCC
FokI 354 CATCC GGATG(9/13)
816 CATCC
Hin1I 298 GGCGCC G[GA]^CG[CT]C
375 GGCGCC
KasI 298 GGCGCC G^GCGCC
375 GGCGCC
KspI 311 CCGCGG CCGC^GG
329 CCGCGG
MaeII 461 ACGT A^CGT
492 ACGT
MamI 78 GATCCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1184 GATGGAAATC
NarI 298 GGCGCC GG^CGCC
375 GGCGCC
PvuII 201 CAGCTG CAG^CTG
634 CAGCTG
SacII 311 CCGCGG CCGC^GG
329 CCGCGG
SfoI 298 GGCGCC GGC^GCC
375 GGCGCC
TaqI 65 TCGA T^CGA
840 TCGA
TthHB8I 65 TCGA T^CGA
840 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AccII AciI AluI AlwNI Aor51HI
ApaI BanI BanII BbvI BcnI
BmyI BsaJI BseRI BsgI BsiYI
BslI BsmAI Bsp1286I BsrFI BsrI
BssKI BstNI BstUI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DsaI
EaeI EarI Eco47III EcoRII EcoT14I
FauI Fnu4HI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MboI MboII MnlI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIV PleI Sau3AI
Sau96I ScrFI StyI TseI TspRI