Restriction map for
pFN21AE3306
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# NO CUT ENZYMES ( 82 enzymes) #
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AccIII AclI AflII AgeI Alw44I
ApaLI AscI AseI AsnI AspI
AviII AvrII BbeI BciVI BclI
BfrI BlnI BsaWI BseAI BsiWI
Bsp1407I BspEI BsrDI BsrGI BssHII
BssSI Bst1107I BstBI BstZ17I CpoI
DraI DraIII DrdI EcoRI EcoRV
EcoT22I FbaI FseI FspI HincII
HindII HindIII HpaI KasI MfeI
MluI MroI MunI NarI NcoI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmeI PshAI PshBI Psp1406I PvuI
RsrII SalI SapI ScaI SexAI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SspBI SspI SwaI Tth111I
XbaI XhoI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AatII 1617 GACGTC GACGT^C
Acc65I 1730 GGTACC G^GTACC
AccI 1279 GTCTAC GT^[AC][GT]AC
AcyI 1617 GACGTC G[GA]^CG[CT]C
AflIII 1784 ACATGT A^C[GA][CT]GT
AocI 2191 CCTGAGG CC^T[ACGT]AGG
ApaI 2286 GGGCCC GGGCC^C
Asp718I 1730 GGTACC G^GTACC
BalI 248 TGGCCA TGG^CCA
BbrPI 2133 CACGTG CAC^GTG
BglI 1637 GCCACGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1457 AGATCT A^GATCT
BlpI 1632 GCTGAGC GC^T[ACGT]AGC
BpmI 654 CTGGAG CTGGAG(16/14)
Bpu1102I 1632 GCTGAGC GC^T[ACGT]AGC
BsaAI 2133 CACGTG [CT]AC^GT[GA]
BsaBI 1458 GATCTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1617 GACGTC G[GA]^CG[CT]C
BsmI 441 GCATTC GAATGC(1/-1)
BspDI 1855 ATCGAT AT^CGAT
BspHI 2021 TCATGA T^CATGA
BspLU11I 1784 ACATGT A^CATGT
BstAPI 1182 GCAGGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1719 GGTGACC G^GT[ACGT]ACC
BstPI 1719 GGTGACC G^GT[ACGT]ACC
BstXI 1772 CCACGGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 2191 CCTGAGG CC^T[ACGT]AGG
CelII 1632 GCTGAGC GC^T[ACGT]AGC
ClaI 1855 ATCGAT AT^CGAT
Eco81I 2191 CCTGAGG CC^T[ACGT]AGG
EcoO65I 1719 GGTGACC G^GT[ACGT]ACC
EcoT14I 1760 CCAAGG C^C[AT][AT]GG
HgaI 1226 GCGTC GACGC(5/10)
Hin1I 1617 GACGTC G[GA]^CG[CT]C
KpnI 1730 GGTACC GGTAC^C
MamI 1458 GATCTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 248 TGGCCA TGG^CCA
MscI 248 TGGCCA TGG^CCA
MseI 103 TTAA T^TAA
PmaCI 2133 CACGTG CAC^GTG
PmlI 2133 CACGTG CAC^GTG
PstI 1210 CTGCAG CTGCA^G
RcaI 2021 TCATGA T^CATGA
SbfI 1209 CCTGCAGG CCTGCA^GG
SphI 330 GCATGC GCATG^C
Sse8387I 1209 CCTGCAGG CCTGCA^GG
StyI 1760 CCAAGG C^C[AT][AT]GG
Tru9I 103 TTAA T^TAA
XcmI 1557 CCAGCTGCTGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 27 enzymes) #
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AcsI 44 AAATTC [GA]^AATT[CT]
108 AAATTC
AhdI 745 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1783 GACATGTGGTC
Aor51HI 1982 AGCGCT AGC^GCT
2062 AGCGCT
ApoI 44 AAATTC [GA]^AATT[CT]
108 AAATTC
AspEI 745 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1783 GACATGTGGTC
BamHI 1595 GGATCC G^GATCC
1687 GGATCC
BbvCI 1371 GCTGAGG CCTCAGC(-5/-2)
2065 GCTGAGG
BfaI 335 CTAG C^TAG
1895 CTAG
BsmBI 701 GAGACG CGTCTC(1/5)
2239 GAGACG
Eam1105I 745 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1783 GACATGTGGTC
Eco47III 1982 AGCGCT AGC^GCT
2062 AGCGCT
KspI 679 CCGCGG CCGC^GG
2282 CCGCGG
MaeI 335 CTAG C^TAG
1895 CTAG
MslI 1464 CATCGTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1773 CACGGCCGTG
NaeI 1203 GCCGGC GCC^GGC
1642 GCCGGC
NgoMIV 1203 GCCGGC G^CCGGC
1642 GCCGGC
NspI 330 GCATGC [GA]CATG^[CT]
1784 ACATGT
PflMI 647 CCAGCCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1026 CCACCTCTTGG
PpuMI 743 GGGACCT [GA]G^G[AT]CC[CT]
1888 GGGACCC
PvuII 1558 CAGCTG CAG^CTG
2215 CAGCTG
SacI 1174 GAGCTC GAGCT^C
1924 GAGCTC
SacII 679 CCGCGG CCGC^GG
2282 CCGCGG
SmaI 1840 CCCGGG CCC^GGG
2047 CCCGGG
StuI 1700 AGGCCT AGG^CCT
2199 AGGCCT
Van91I 647 CCAGCCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1026 CCACCTCTTGG
XmaCI 1840 CCCGGG C^CCGGG
2047 CCCGGG
XmaI 1840 CCCGGG C^CCGGG
2047 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI Asp700I AspHI AvaI AvaII
BanI BanII BbsI BbvI BcgI
BcnI BmyI BpuAI BsaI BsaJI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrFI BsrI BssKI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EagI EarI
EclXI Eco52I EcoNI EcoO109I EcoRII
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeII MaeIII
MboI MboII MflI MnlI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PleI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI TaqI TfiI TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII XmnI