Restriction map for
pFN21AE4225
##################################
# NO CUT ENZYMES ( 96 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AgeI Alw44I Aor51HI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspI AviII AvrII BanI
BbeI BciVI BclI BfrI BglII
BlnI BsaWI BseAI BsiWI BsmI
Bsp1407I BspDI BspEI BspLU11I BsrGI
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstZ17I ClaI CpoI DraI
DraIII Eco47III EcoO65I EcoRI EcoRV
EcoT22I FbaI FspI HincII HindII
HpaI KasI KpnI MfeI MluI
MroI MunI NarI NcoI NdeI
NheI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmeI PshAI
PshBI Psp1406I PvuI RsrII SalI
SfoI SfuI SgrAI SnaBI SpeI
SphI SspBI SspI SwaI TfiI
Tsp509I Tth111I XbaI XcmI XhoI
XmnI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AccI 565 GTAGAC GT^[AC][GT]AC
AcyI 2338 GACGCC G[GA]^CG[CT]C
AflIII 254 ACACGT A^C[GA][CT]GT
AocI 631 CCTGAGG CC^T[ACGT]AGG
BalI 113 TGGCCA TGG^CCA
BamHI 260 GGATCC G^GATCC
BbrPI 255 CACGTG CAC^GTG
BbsI 1360 GAAGAC GAAGAC(2/6)
BbvCI 87 CCTCAGC CCTCAGC(-5/-2)
BcgI 464 GCAGGGACATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpuAI 1360 GAAGAC GAAGAC(2/6)
BsaAI 255 CACGTG [CT]AC^GT[GA]
BsaBI 1134 GATCGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 2338 GACGCC G[GA]^CG[CT]C
BsaI 1381 GAGACC GGTCTC(1/5)
BspHI 545 TCATGA T^CATGA
BsrBI 2299 GAGCGG CCGCTC(-3/-3)
BsrDI 1989 CATTGC GCAATG(2/0)
BssSI 493 CACGAG CACGAG(-5/-1)
Bsu36I 631 CCTGAGG CC^T[ACGT]AGG
Eco81I 631 CCTGAGG CC^T[ACGT]AGG
EcoT14I 2309 CCAAGG C^C[AT][AT]GG
FseI 2303 GGCCGGCC GGCCGG^CC
Hin1I 2338 GACGCC G[GA]^CG[CT]C
HindIII 792 AAGCTT A^AGCTT
KspI 1623 CCGCGG CCGC^GG
MaeIII 1207 GTGAC ^GT[ACGT]AC
MamI 1134 GATCGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 113 TGGCCA TGG^CCA
MscI 113 TGGCCA TGG^CCA
MseI 1574 TTAA T^TAA
NaeI 2304 GCCGGC GCC^GGC
NgoMIV 2304 GCCGGC G^CCGGC
NotI 1628 GCGGCCGC GC^GGCCGC
PmaCI 255 CACGTG CAC^GTG
PmlI 255 CACGTG CAC^GTG
RcaI 545 TCATGA T^CATGA
SacII 1623 CCGCGG CCGC^GG
SbfI 2241 CCTGCAGG CCTGCA^GG
SexAI 1052 ACCAGGT A^CC[AT]GGT
SmaI 869 CCCGGG CCC^GGG
Sse8387I 2241 CCTGCAGG CCTGCA^GG
StuI 641 AGGCCT AGG^CCT
StyI 2309 CCAAGG C^C[AT][AT]GG
Tru9I 1574 TTAA T^TAA
Tsp45I 1207 GTGAC ^GT[GC]AC
XmaCI 869 CCCGGG C^CCGGG
XmaI 869 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AhdI 151 GACTCCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1363 GACTTCGTGTC
ApaI 1427 GGGCCC GGGCC^C
2121 GGGCCC
AspEI 151 GACTCCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1363 GACTTCGTGTC
AvaI 715 CTCGGG C^[CT]CG[GA]G
869 CCCGGG
BfaI 205 CTAG C^TAG
1192 CTAG
BglI 43 GCCCTGTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1612 GCCCATCTGGC
BlpI 1872 GCTCAGC GC^T[ACGT]AGC
2160 GCTGAGC
Bpu1102I 1872 GCTCAGC GC^T[ACGT]AGC
2160 GCTGAGC
BsmBI 1411 GAGACG CGTCTC(1/5)
1759 GAGACG
BsoBI 715 CTCGGG C^[CT]CG[GA]G
869 CCCGGG
BssHII 1809 GCGCGC G^CGCGC
2278 GCGCGC
BstYI 260 GGATCC [GA]^GATC[CT]
2003 AGATCC
CelII 1872 GCTCAGC GC^T[ACGT]AGC
2160 GCTGAGC
DrdI 397 GACATGCCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1030 GACAAGCTGGTC
Eam1105I 151 GACTCCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1363 GACTTCGTGTC
HphI 939 GGTGA GGTGA(8/7)
1926 GGTGA
MaeI 205 CTAG C^TAG
1192 CTAG
MflI 260 GGATCC [GA]^GATC[CT]
2003 AGATCC
NspI 398 ACATGC [GA]CATG^[CT]
743 ACATGC
ScaI 416 AGTACT AGT^ACT
986 AGTACT
SfiI 42 GGCCCTGTCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
1611 GGCCCATCTGGCC
XhoII 260 GGATCC [GA]^GATC[CT]
2003 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 92 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AspHI AvaII BanII BbvI
BcnI BmyI BpmI BsaJI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BspMI BsrFI
BsrI BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EagI
EarI EclXI Eco52I EcoNI EcoO109I
EcoRII FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII ItaI Ksp632I MaeII
MboI MboII MnlI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PflMI PleI
PpuMI PstI PvuII RsaI SacI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI TaqI TseI TspRI
TthHB8I Van91I