Restriction map for
pFN21AB6481
##################################
# NO CUT ENZYMES ( 134 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AflII AflIII Alw44I AocI Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspI AviII AvrII
BalI BamHI BbsI BbvCI BcgI
BciVI BclI BfaI BfrI BglII
BlnI BlpI Bpu1102I BpuAI BsaBI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I Bsu36I
CelII ClaI CpoI DraI DraIII
DrdI Eco47III Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FbaI
FseI FspI HindIII HpaI KpnI
KspI MaeI MamI MfeI MluI
MluNI MscI MseI MunI NcoI
NdeI NheI NlaIII NotI NruI
NsiI NspI NspV PacI PflFI
PflMI PmeI PshAI PshBI Psp1406I
PstI PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfcI SfiI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI StyI SwaI
TfiI Tru9I Tsp509I Tth111I Van91I
XbaI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AccIII 120 TCCGGA T^CCGGA
AgeI 359 ACCGGT A^CCGGT
AhdI 343 GACCATCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 208 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 498 GGGCCC GGGCC^C
AspEI 343 GACCATCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 679 CTCGAG C^[CT]CG[GA]G
BbeI 367 GGCGCC GGCGC^C
BbrPI 199 CACGTG CAC^GTG
BglI 538 GCCTGGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BseAI 120 TCCGGA T^CCGGA
BsgI 514 CTGCAC GTGCAG(16/14)
BsiEI 411 CGGCCG CG[GA][CT]^CG
BsoBI 679 CTCGAG C^[CT]CG[GA]G
BspEI 120 TCCGGA T^CCGGA
BsrI 856 ACTGG ACTGG(1/-1)
BstXI 548 CCAACGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 275 AGATCC [GA]^GATC[CT]
DsaI 50 CCGTGG C^C[GA][CT]GG
EagI 411 CGGCCG C^GGCCG
Eam1105I 343 GACCATCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 124 GAAGAG CTCTTC(1/4)
EclXI 411 CGGCCG C^GGCCG
Eco52I 411 CGGCCG C^GGCCG
HaeII 367 GGCGCC [GA]GCGC^[CT]
HincII 460 GTCAAC GT[CT]^[GA]AC
HindII 460 GTCAAC GT[CT]^[GA]AC
KasI 367 GGCGCC G^GCGCC
Ksp632I 124 GAAGAG CTCTTC(1/4)
MaeIII 652 GTCAC ^GT[ACGT]AC
MflI 275 AGATCC [GA]^GATC[CT]
MroI 120 TCCGGA T^CCGGA
MslI 855 CACTGGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 367 GGCGCC GG^CGCC
PaeR7I 679 CTCGAG C^TCGAG
PinAI 359 ACCGGT A^CCGGT
PmaCI 199 CACGTG CAC^GTG
PmlI 199 CACGTG CAC^GTG
PpuMI 317 GGGACCC [GA]G^G[AT]CC[CT]
SfoI 367 GGCGCC GGC^GCC
SmlI 679 CTCGAG C^T[CT][GA]AG
Tsp45I 652 GTCAC ^GT[GC]AC
XcmI 485 CCATCCCCAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 679 CTCGAG C^TCGAG
XhoII 275 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AfaI 30 GTAC GT^AC
363 GTAC
AlwI 276 GATCC GGATC(4/5)
443 GGATC
AvaII 204 GGACC G^G[AT]CC
318 GGACC
BanI 356 GGCACC G^G[CT][GA]CC
367 GGCGCC
BanII 498 GGGCCC G[GA]GC[CT]^C
676 GGGCTC
BpmI 894 CTCCAG CTGGAG(16/14)
778 CTGGAG
BsaAI 199 CACGTG [CT]AC^GT[GA]
664 CACGTA
BsaI 127 GAGACC GGTCTC(1/5)
341 GAGACC
BsaWI 120 TCCGGA [AT]^CCGG[AT]
359 ACCGGT
BsmAI 127 GAGAC GTCTC(1/5)
341 GAGAC
DdeI 323 CTGAG C^T[ACGT]AG
656 CTGAG
HgaI 10 GCGTC GACGC(5/10)
425 GCGTC
HinfI 97 GAGTC G^A[ACGT]TC
509 GAGTC
MboII 124 GAAGA GAAGA(8/7)
257 TCTTC
MspA1I 730 CAGCTG C[AC]G^C[GT]G
792 CAGCTG
PleI 97 GAGTC GAGTC(4/5)
509 GAGTC
PvuII 730 CAGCTG CAG^CTG
792 CAGCTG
RsaI 30 GTAC GT^AC
363 GTAC
#########################################
# 3 OR MORE CUT ENZYMES ( 61 enzymes) #
#########################################
AccII AciI AcyI AluI AspHI
BbvI BcnI BmyI BsaHI BsaJI
BseRI BsiHKAI BsiYI BslI BsmFI
Bsp1286I BsrFI BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr10I Cfr13I
DpnII DraII EaeI EcoO109I EcoRII
FauI Fnu4HI FokI HaeIII HapII
HhaI Hin1I HinP1I HpaII HphI
ItaI MaeII MboI MnlI MspI
MvaI MvnI MwoI NaeI NciI
NdeII NgoMIV NlaIV Sau3AI Sau96I
ScrFI SfaNI TaqI TseI TspRI
TthHB8I