Restriction map for
pFN21AB7704
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# NO CUT ENZYMES ( 126 enzymes) #
##################################
AatII AccII AclI AflIII AgeI
AlwNI AocI Aor51HI ApaI AscI
AseI AsnI AspI AviII AvrII
BamHI BanII BbrPI BbvCI BcgI
BciVI BclI BcnI BglII BlnI
BsaAI BsaBI BsaI BsgI BsiWI
BsmBI BsmI Bsp1407I BspDI BspLU11I
BspMI BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
Cfr10I ClaI CpoI DraI DraIII
DrdI EagI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoT22I
FauI FbaI FseI FspI HindIII
HpaI KspI MamI MfeI MluI
MunI MvnI NaeI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RsrII SacI SacII SapI SbfI
ScaI SexAI SfiI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tsp45I
Tth111I Van91I XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 64 enzymes) #
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Acc65I 1156 GGTACC G^GTACC
AccI 103 GTCGAC GT^[AC][GT]AC
AccIII 701 TCCGGA T^CCGGA
AciI 199 GCGG CCGC(-3/-1)
AcyI 410 GGCGCC G[GA]^CG[CT]C
AflII 676 CTTAAG C^TTAAG
AhdI 106 GACCGTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 546 GTGCAC G^TGCAC
ApaLI 546 GTGCAC G^TGCAC
Asp700I 693 GAAAAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1156 GGTACC G^GTACC
AspEI 106 GACCGTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 546 GTGCAC G[AT]GC[AT]^C
AvaII 720 GGTCC G^G[AT]CC
BalI 1134 TGGCCA TGG^CCA
BbeI 410 GGCGCC GGCGC^C
BbsI 1057 GAAGAC GAAGAC(2/6)
BfrI 676 CTTAAG C^TTAAG
BglI 402 GCCTGATGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 451 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 451 GCTAAGC GC^T[ACGT]AGC
BpuAI 1057 GAAGAC GAAGAC(2/6)
BsaHI 410 GGCGCC G[GA]^CG[CT]C
BsaWI 701 TCCGGA [AT]^CCGG[AT]
BseAI 701 TCCGGA T^CCGGA
BsiEI 105 CGACCG CG[GA][CT]^CG
BsiHKAI 546 GTGCAC G[AT]GC[AT]^C
BsmAI 798 GAGAC GTCTC(1/5)
BspEI 701 TCCGGA T^CCGGA
BspHI 1256 TCATGA T^CATGA
BstYI 1248 GGATCT [GA]^GATC[CT]
CelII 451 GCTAAGC GC^T[ACGT]AGC
DsaI 108 CCGTGG C^C[GA][CT]GG
EaeI 1134 TGGCCA [CT]^GGCC[GA]
Eam1105I 106 GACCGTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT14I 863 CCAAGG C^C[AT][AT]GG
HapII 702 CCGG C^CGG
HgaI 312 GCGTC GACGC(5/10)
Hin1I 410 GGCGCC G[GA]^CG[CT]C
HpaII 702 CCGG C^CGG
HphI 559 GGTGA GGTGA(8/7)
KasI 410 GGCGCC G^GCGCC
KpnI 1156 GGTACC GGTAC^C
MflI 1248 GGATCT [GA]^GATC[CT]
MluNI 1134 TGGCCA TGG^CCA
MroI 701 TCCGGA T^CCGGA
MscI 1134 TGGCCA TGG^CCA
MseI 677 TTAA T^TAA
MslI 1257 CATGAAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 702 CCGG C^CGG
NarI 410 GGCGCC GG^CGCC
NspI 327 ACATGC [GA]CATG^[CT]
PpuMI 719 AGGTCCC [GA]G^G[AT]CC[CT]
PstI 1111 CTGCAG CTGCA^G
RcaI 1256 TCATGA T^CATGA
SalI 103 GTCGAC G^TCGAC
SfoI 410 GGCGCC GGC^GCC
StyI 863 CCAAGG C^C[AT][AT]GG
TaqI 104 TCGA T^CGA
Tru9I 677 TTAA T^TAA
TthHB8I 104 TCGA T^CGA
XbaI 982 TCTAGA T^CTAGA
XhoII 1248 GGATCT [GA]^GATC[CT]
XmnI 693 GAAAAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AcsI 95 AAATTC [GA]^AATT[CT]
1178 AAATTC
AlwI 601 GATCC GGATC(4/5)
1248 GGATC
ApoI 95 AAATTC [GA]^AATT[CT]
1178 AAATTC
AvaI 10 CCCGAG C^[CT]CG[GA]G
909 CTCGGG
BmyI 6 GTGCCC G[AGT]GC[ACT]^C
546 GTGCAC
BseRI 1069 GAGGAG GAGGAG(10/8)
1072 GAGGAG
BsiYI 924 CCACAAGCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1137 CCAGTATAGGG
BslI 924 CCACAAGCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1137 CCAGTATAGGG
BsoBI 10 CCCGAG C^[CT]CG[GA]G
909 CTCGGG
Bsp1286I 6 GTGCCC G[AGT]GC[ACT]^C
546 GTGCAC
BsrDI 144 CATTGC GCAATG(2/0)
846 CATTGC
Cac8I 148 GCTGGC GC[ACGT]^[ACGT]GC
1113 GCAGGC
Cfr13I 228 GGCCC G^G[ACGT]CC
720 GGTCC
DpnII 601 GATC ^GATC
1249 GATC
DraII 227 AGGCCCC [GA]G^G[ACGT]CC[CT]
719 AGGTCCC
EarI 1066 GAAGAG CTCTTC(1/4)
1090 GAAGAG
EcoO109I 227 AGGCCCC [GA]G^G[ACGT]CC[CT]
719 AGGTCCC
EcoRV 562 GATATC GAT^ATC
903 GATATC
HincII 103 GTCGAC GT[CT]^[GA]AC
460 GTTGAC
HindII 103 GTCGAC GT[CT]^[GA]AC
460 GTTGAC
Ksp632I 1066 GAAGAG CTCTTC(1/4)
1090 GAAGAG
MaeII 354 ACGT A^CGT
779 ACGT
MboI 601 GATC ^GATC
1249 GATC
MspA1I 587 CAGCTG C[AC]G^C[GT]G
1108 CAGCTG
NdeII 601 GATC ^GATC
1249 GATC
PvuII 587 CAGCTG CAG^CTG
1108 CAGCTG
Sau3AI 601 GATC ^GATC
1249 GATC
Sau96I 228 GGCCC G^G[ACGT]CC
720 GGTCC
SfcI 1111 CTGCAG C^T[GA][CT]AG
1354 CTACAG
TspRI 549 CACTG CA[GC]TG[ACGT][ACGT]^
975 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 38 enzymes) #
#########################################
AfaI AluI BanI BbvI BfaI
BpmI BsaJI BsmFI BsrI BssKI
BstF5I BstNI CfoI DdeI EcoRII
Fnu4HI FokI HaeII HaeIII HhaI
HinP1I HinfI ItaI MaeI MaeIII
MboII MnlI MvaI MwoI NlaIII
NlaIV RsaI ScrFI SfaNI SmlI
TfiI TseI Tsp509I