Restriction map for
pFN21AE9256
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# NO CUT ENZYMES ( 101 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AflII AflIII AgeI AhdI AocI
Aor51HI ApaI ApoI AscI AseI
AsnI Asp718I AspEI AspI AviII
BamHI BbrPI BcgI BciVI BclI
BfrI BglI BsaAI BsaWI BseAI
BsiWI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrDI BsrGI Bst1107I
BstEII BstPI BstZ17I Bsu36I ClaI
CpoI DraI DrdI Eam1105I Eco47III
Eco81I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HindIII HpaI
KpnI KspI MaeII MfeI MluI
MroI MunI NaeI NdeI NgoMIV
NheI NotI NruI NsiI PacI
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SbfI SexAI SfiI
SgrAI SnaBI SpeI Sse8387I SspBI
SspI SwaI Tsp509I Tth111I XbaI
XcmI
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# ONE CUT ENZYMES ( 32 enzymes) #
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AccI 298 GTCGAC GT^[AC][GT]AC
Asp700I 51 GAACAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII 757 CCTAGG C^CTAGG
BfaI 758 CTAG C^TAG
BglII 43 AGATCT A^GATCT
BlnI 757 CCTAGG C^CTAGG
BlpI 1360 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1360 GCTCAGC GC^T[ACGT]AGC
BsaBI 1920 GATCGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 892 GAGACG CGTCTC(1/5)
BstBI 177 TTCGAA TT^CGAA
CelII 1360 GCTCAGC GC^T[ACGT]AGC
DraIII 2050 CACACCGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 1271 CGGCCG C^GGCCG
EclXI 1271 CGGCCG C^GGCCG
Eco52I 1271 CGGCCG C^GGCCG
HincII 298 GTCGAC GT[CT]^[GA]AC
HindII 298 GTCGAC GT[CT]^[GA]AC
MaeI 758 CTAG C^TAG
MamI 1920 GATCGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 674 TTAA T^TAA
NspV 177 TTCGAA TT^CGAA
SalI 298 GTCGAC G^TCGAC
ScaI 1277 AGTACT AGT^ACT
SfuI 177 TTCGAA TT^CGAA
SmaI 1076 CCCGGG CCC^GGG
StuI 1892 AGGCCT AGG^CCT
TfiI 1007 GATTC G^A[AT]TC
Tru9I 674 TTAA T^TAA
XmaCI 1076 CCCGGG C^CCGGG
XmaI 1076 CCCGGG C^CCGGG
XmnI 51 GAACAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 28 enzymes) #
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AcyI 872 GGCGCC G[GA]^CG[CT]C
2084 GGCGCC
Alw44I 1333 GTGCAC G^TGCAC
2074 GTGCAC
ApaLI 1333 GTGCAC G^TGCAC
2074 GTGCAC
BalI 932 TGGCCA TGG^CCA
1655 TGGCCA
BbeI 872 GGCGCC GGCGC^C
2084 GGCGCC
BbsI 4 GAAGAC GAAGAC(2/6)
994 GAAGAC
BbvCI 2109 CCTCAGC CCTCAGC(-5/-2)
1312 GCTGAGG
BpuAI 4 GAAGAC GAAGAC(2/6)
994 GAAGAC
BsaHI 872 GGCGCC G[GA]^CG[CT]C
2084 GGCGCC
BsiEI 300 CGACCG CG[GA][CT]^CG
1271 CGGCCG
BsrFI 1909 ACCGGC [GA]^CCGG[CT]
1946 ACCGGC
BssHII 797 GCGCGC G^CGCGC
1725 GCGCGC
BssSI 1168 CACGAG CACGAG(-5/-1)
1672 CACGAG
Cfr10I 1909 ACCGGC [GA]^CCGG[CT]
1946 ACCGGC
EarI 2131 CTCTTC CTCTTC(1/4)
841 GAAGAG
EcoNI 189 CCTCATCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1668 CCTGCACGAGG
Hin1I 872 GGCGCC G[GA]^CG[CT]C
2084 GGCGCC
KasI 872 GGCGCC G^GCGCC
2084 GGCGCC
Ksp632I 2131 CTCTTC CTCTTC(1/4)
841 GAAGAG
MluNI 932 TGGCCA TGG^CCA
1655 TGGCCA
MscI 932 TGGCCA TGG^CCA
1655 TGGCCA
NarI 872 GGCGCC GG^CGCC
2084 GGCGCC
PaeR7I 242 CTCGAG C^TCGAG
2125 CTCGAG
PpuMI 1199 GGGTCCT [GA]G^G[AT]CC[CT]
1246 GGGTCCT
SapI 841 GAAGAGC GCTCTTC(1/4)
2130 GCTCTTC
SfoI 872 GGCGCC GGC^GCC
2084 GGCGCC
SphI 143 GCATGC GCATG^C
1301 GCATGC
XhoI 242 CTCGAG C^TCGAG
2125 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 97 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AspHI AvaI AvaII BanI
BanII BbvI BcnI BmyI BpmI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
MaeIII MboI MboII MflI MnlI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PflMI PleI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TseI Tsp45I TspRI TthHB8I
Van91I XhoII