Restriction map for
pFN21AE4220
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# NO CUT ENZYMES ( 109 enzymes) #
##################################
AatII AclI AcsI AgeI AhdI
Aor51HI ApaI ApoI AscI AseI
AsnI Asp700I AspEI AspI AviII
AvrII BalI BamHI BbeI BbrPI
BbsI BbvCI BciVI BlnI BpuAI
BsaAI BsaBI BsaI BsiEI BsiWI
BspDI BspHI BspLU11I BssHII BssSI
Bst1107I BstBI BstZ17I ClaI CpoI
DraI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoRI EcoRV EcoT22I
FseI FspI HinfI HpaI KasI
MamI MfeI MluI MluNI MscI
MunI NarI NcoI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PleI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI RcaI RsrII SacI
SalI SbfI SexAI SfcI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspI SwaI
TfiI Tsp509I Tth111I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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Acc65I 476 GGTACC G^GTACC
AccI 1792 GTAGAC GT^[AC][GT]AC
AccIII 443 TCCGGA T^CCGGA
AflIII 152 ACGTGT A^C[GA][CT]GT
Alw44I 1561 GTGCAC G^TGCAC
AocI 1288 CCTGAGG CC^T[ACGT]AGG
ApaLI 1561 GTGCAC G^TGCAC
Asp718I 476 GGTACC G^GTACC
BcgI 1908 CGACAGCGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 538 CTAG C^TAG
BlpI 1417 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1417 GCTCAGC GC^T[ACGT]AGC
BseAI 443 TCCGGA T^CCGGA
BsmAI 1034 GTCTC GTCTC(1/5)
BsmBI 1033 CGTCTC CGTCTC(1/5)
BsmI 1009 GAATGC GAATGC(1/-1)
Bsp1407I 116 TGTACA T^GTACA
BspEI 443 TCCGGA T^CCGGA
BsrGI 116 TGTACA T^GTACA
BstEII 699 GGTGACC G^GT[ACGT]ACC
BstPI 699 GGTGACC G^GT[ACGT]ACC
Bsu36I 1288 CCTGAGG CC^T[ACGT]AGG
CelII 1417 GCTCAGC GC^T[ACGT]AGC
DrdI 43 GACGCACTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 1198 CGGCCA [CT]^GGCC[GA]
Eco81I 1288 CCTGAGG CC^T[ACGT]AGG
EcoO65I 699 GGTGACC G^GT[ACGT]ACC
HaeII 1039 AGCGCC [GA]GCGC^[CT]
HincII 737 GTCAAC GT[CT]^[GA]AC
HindII 737 GTCAAC GT[CT]^[GA]AC
HindIII 1840 AAGCTT A^AGCTT
KpnI 476 GGTACC GGTAC^C
KspI 1518 CCGCGG CCGC^GG
MaeI 538 CTAG C^TAG
MroI 443 TCCGGA T^CCGGA
NspI 718 ACATGC [GA]CATG^[CT]
PpuMI 212 GGGACCC [GA]G^G[AT]CC[CT]
SacII 1518 CCGCGG CCGC^GG
SapI 597 GCTCTTC GCTCTTC(1/4)
ScaI 1104 AGTACT AGT^ACT
SspBI 116 TGTACA T^GTACA
StuI 161 AGGCCT AGG^CCT
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# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccII 568 CGCG CG^CG
1519 CGCG
AcyI 1822 GGCGTC G[GA]^CG[CT]C
1938 GGCGTC
AflII 316 CTTAAG C^TTAAG
1108 CTTAAG
AlwI 1047 GATCC GGATC(4/5)
1980 GATCC
AvaI 170 CCCGAG C^[CT]CG[GA]G
1169 CCCGAG
BcnI 1893 CCCGG CC^[GC]GG
2100 CCGGG
BfrI 316 CTTAAG C^TTAAG
1108 CTTAAG
BglII 872 AGATCT A^GATCT
1334 AGATCT
BsaHI 1822 GGCGTC G[GA]^CG[CT]C
1938 GGCGTC
BsaWI 443 TCCGGA [AT]^CCGG[AT]
1058 ACCGGA
BsoBI 170 CCCGAG C^[CT]CG[GA]G
1169 CCCGAG
BspMI 1371 ACCTGC ACCTGC(4/8)
1565 ACCTGC
BsrDI 1225 CATTGC GCAATG(2/0)
581 GCAATG
BstUI 568 CGCG CG^CG
1519 CGCG
BstXI 1369 CCACCTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1964 CCATTGGCATGG
DraIII 148 CACAACGTG CAC[ACGT][ACGT][ACGT]^GTG
558 CACAGAGTG
DsaI 1518 CCGCGG C^C[GA][CT]GG
2130 CCGTGG
EarI 598 CTCTTC CTCTTC(1/4)
694 GAAGAG
Hin1I 1822 GGCGTC G[GA]^CG[CT]C
1938 GGCGTC
Ksp632I 598 CTCTTC CTCTTC(1/4)
694 GAAGAG
MvnI 568 CGCG CG^CG
1519 CGCG
NaeI 1534 GCCGGC GCC^GGC
2041 GCCGGC
NciI 1893 CCCGG CC^[GC]GG
2100 CCGGG
NgoMIV 1534 GCCGGC G^CCGGC
2041 GCCGGC
PflMI 723 CCAACGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1430 CCACCCGCTGG
TaqI 1235 TCGA T^CGA
1604 TCGA
TthHB8I 1235 TCGA T^CGA
1604 TCGA
Van91I 723 CCAACGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1430 CCACCCGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 79 enzymes) #
#########################################
AciI AfaI AluI AlwNI AspHI
AvaII BanI BanII BbvI BclI
BglI BmyI BpmI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmFI
Bsp1286I BsrBI BsrFI BsrI BssKI
BstAPI BstF5I BstNI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII EcoO109I EcoRII EcoT14I FauI
FbaI Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HpaII HphI
ItaI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NdeII NlaIII
NlaIV PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SmlI StyI Tru9I
TseI Tsp45I TspRI XhoII