Restriction map for
pFN21AE4273
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# NO CUT ENZYMES ( 102 enzymes) #
##################################
AatII AccII AccIII AciI AflII
AgeI Alw44I Aor51HI ApaI ApaLI
AscI AvaI AviII AvrII BalI
BamHI BbeI BbrPI BciVI BcnI
BfrI BglII BlnI BsaI BsaWI
BseAI BsiEI BsiWI BsmI BsoBI
BspDI BspEI BsrBI BsrFI BssHII
BssSI Bst1107I BstBI BstUI BstYI
BstZ17I Cfr10I ClaI CpoI DrdI
EagI EclXI Eco47III Eco52I EcoNI
EcoT22I FauI FseI FspI HapII
HpaII KasI KspI MfeI MflI
MluI MluNI MroI MscI MspI
MunI MvnI NaeI NarI NciI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshAI PvuI RsrII
SacI SacII SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SwaI XhoI XhoII
XmaCI XmaI
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# ONE CUT ENZYMES ( 48 enzymes) #
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Acc65I 1673 GGTACC G^GTACC
AclI 1006 AACGTT AA^CGTT
AcyI 1971 GACGCC G[GA]^CG[CT]C
AhdI 3348 GACATTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 3047 CAGACACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 479 CCTTAGG CC^T[ACGT]AGG
Asp718I 1673 GGTACC G^GTACC
AspEI 3348 GACATTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1766 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanII 466 GGGCTC G[GA]GC[CT]^C
BbvCI 534 GCTGAGG CCTCAGC(-5/-2)
BcgI 2798 CGATAGAATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 730 GCCATTTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 2782 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2782 GCTGAGC GC^T[ACGT]AGC
BsaAI 838 TACGTG [CT]AC^GT[GA]
BsaHI 1971 GACGCC G[GA]^CG[CT]C
BseRI 488 CTCCTC GAGGAG(10/8)
BspHI 1877 TCATGA T^CATGA
BspMI 2320 GCAGGT ACCTGC(4/8)
Bsu36I 479 CCTTAGG CC^T[ACGT]AGG
CelII 2782 GCTGAGC GC^T[ACGT]AGC
CfoI 3139 GCGC GCG^C
DraII 483 AGGACCT [GA]G^G[ACGT]CC[CT]
DraIII 341 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 788 CCATGG C^C[GA][CT]GG
EaeI 1548 TGGCCG [CT]^GGCC[GA]
Eam1105I 3348 GACATTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 479 CCTTAGG CC^T[ACGT]AGG
EcoO109I 483 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoRV 2439 GATATC GAT^ATC
HaeII 3138 GGCGCT [GA]GCGC^[CT]
HhaI 3139 GCGC GCG^C
Hin1I 1971 GACGCC G[GA]^CG[CT]C
HinP1I 3139 GCGC G^CGC
HpaI 1461 GTTAAC GTT^AAC
KpnI 1673 GGTACC GGTAC^C
NcoI 788 CCATGG C^CATGG
NdeI 905 CATATG CA^TATG
PflFI 1766 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 483 AGGACCT [GA]G^G[AT]CC[CT]
Psp1406I 1006 AACGTT AA^CGTT
PstI 2420 CTGCAG CTGCA^G
RcaI 1877 TCATGA T^CATGA
SphI 2986 GCATGC GCATG^C
StuI 619 AGGCCT AGG^CCT
Tth111I 1766 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 2503 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 38 enzymes) #
###################################
AflIII 1032 ACATGT A^C[GA][CT]GT
2373 ACATGT
AlwI 1387 GATCC GGATC(4/5)
529 GGATC
AseI 3177 ATTAAT AT^TAAT
3207 ATTAAT
AsnI 3177 ATTAAT AT^TAAT
3207 ATTAAT
AvaII 484 GGACC G^G[AT]CC
497 GGTCC
BanI 1673 GGTACC G^G[CT][GA]CC
2480 GGTGCC
BpmI 1859 CTCCAG CTGGAG(16/14)
2177 CTGGAG
BsaBI 1615 GATTTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2668 GATGAACATC
BsmBI 1969 GAGACG CGTCTC(1/5)
2981 GAGACG
Bsp1407I 1890 TGTACA T^GTACA
2754 TGTACA
BspLU11I 1032 ACATGT A^CATGT
2373 ACATGT
BsrDI 2787 GCAATG GCAATG(2/0)
2939 GCAATG
BsrGI 1890 TGTACA T^GTACA
2754 TGTACA
BstEII 807 GGTTACC G^GT[ACGT]ACC
1260 GGTTACC
BstPI 807 GGTTACC G^GT[ACGT]ACC
1260 GGTTACC
BstXI 510 CCATCGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2104 CCATTGGAGTGG
Cfr13I 484 GGACC G^G[ACGT]CC
497 GGTCC
EcoO65I 807 GGTTACC G^GT[ACGT]ACC
1260 GGTTACC
EcoRI 441 GAATTC G^AATTC
814 GAATTC
EcoT14I 788 CCATGG C^C[AT][AT]GG
2078 CCTTGG
HgaI 1971 GACGC GACGC(5/10)
2983 GACGC
HindIII 100 AAGCTT A^AGCTT
1555 AAGCTT
MamI 1615 GATTTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2668 GATGAACATC
MspA1I 2780 CAGCTG C[AC]G^C[GT]G
2918 CAGCTG
PflMI 1624 CCAGCCATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2971 CCAAGAATTGG
PleI 1857 GACTC GAGTC(4/5)
115 GAGTC
PshBI 3177 ATTAAT AT^TAAT
3207 ATTAAT
PvuII 2780 CAGCTG CAG^CTG
2918 CAGCTG
SalI 1237 GTCGAC G^TCGAC
2954 GTCGAC
SapI 468 GCTCTTC GCTCTTC(1/4)
3197 GCTCTTC
Sau96I 484 GGACC G^G[ACGT]CC
497 GGTCC
ScaI 1217 AGTACT AGT^ACT
1586 AGTACT
SmlI 646 CTTGAG C^T[CT][GA]AG
778 CTCAAG
SspBI 1890 TGTACA T^GTACA
2754 TGTACA
SspI 224 AATATT AAT^ATT
379 AATATT
StyI 788 CCATGG C^C[AT][AT]GG
2078 CCTTGG
Van91I 1624 CCAGCCATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2971 CCAAGAATTGG
XcmI 218 CCAAACAATATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2069 CCAGTGTTCCCTTGG
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# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
#########################################
AccI AcsI AfaI AluI ApoI
Asp700I AspHI BbsI BbvI BclI
BfaI BmyI BpuAI BsaJI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrI BssKI BstAPI BstF5I
BstNI Cac8I DdeI DpnII DraI
EarI EcoRII FbaI Fnu4HI FokI
HaeIII HincII HindII HinfI HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MnlI MseI MslI
MvaI MwoI NdeII NlaIII NlaIV
NspI RsaI Sau3AI ScrFI SfaNI
SfcI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XmnI