Restriction map for
pFN21AE3807
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# NO CUT ENZYMES ( 167 enzymes) #
##################################
AatII AccIII AclI AcyI AflII
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I AspEI AspHI AspI
AvaI AviII AvrII BamHI BanII
BbeI BbrPI BcgI BciVI BcnI
BfrI BglI BglII BlnI BlpI
BmyI BpmI Bpu1102I BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BsgI
BsiEI BsiHKAI BsiWI BsmBI BsmI
BsoBI Bsp1286I BspDI BspEI BspLU11I
BspMI BsrBI BsrDI BsrFI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraII DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FseI
FspI HaeII HapII HgaI Hin1I
HindIII HpaI HpaII KasI KspI
MaeII MamI MfeI MluI MroI
MslI MspI MunI MwoI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspI
StuI StyI SwaI TaqI Tth111I
TthHB8I Van91I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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Acc65I 127 GGTACC G^GTACC
AccI 555 GTCTAC GT^[AC][GT]AC
AccII 425 CGCG CG^CG
AcsI 912 GAATTT [GA]^AATT[CT]
AluI 72 AGCT AG^CT
AlwI 3 GGATC GGATC(4/5)
AlwNI 464 CAGCCGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 912 GAATTT [GA]^AATT[CT]
Asp718I 127 GGTACC G^GTACC
AvaII 404 GGTCC G^G[AT]CC
BalI 251 TGGCCA TGG^CCA
BanI 127 GGTACC G^G[CT][GA]CC
BbvCI 474 CCTCAGC CCTCAGC(-5/-2)
BfaI 70 CTAG C^TAG
BseRI 702 GAGGAG GAGGAG(10/8)
BsmFI 405 GTCCC GGGAC(10/14)
Bsp1407I 107 TGTACA T^GTACA
BspHI 510 TCATGA T^CATGA
BsrGI 107 TGTACA T^GTACA
BstUI 425 CGCG CG^CG
BstYI 3 GGATCT [GA]^GATC[CT]
CfoI 426 GCGC GCG^C
Cfr13I 404 GGTCC G^G[ACGT]CC
DraI 712 TTTAAA TTT^AAA
DraIII 394 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 389 CTCTTC CTCTTC(1/4)
FauI 423 CCCGC CCCGC(4/6)
HhaI 426 GCGC GCG^C
HinP1I 426 GCGC G^CGC
KpnI 127 GGTACC GGTAC^C
Ksp632I 389 CTCTTC CTCTTC(1/4)
MaeI 70 CTAG C^TAG
MflI 3 GGATCT [GA]^GATC[CT]
MluNI 251 TGGCCA TGG^CCA
MscI 251 TGGCCA TGG^CCA
MseI 713 TTAA T^TAA
MspA1I 467 CCGCTG C[AC]G^C[GT]G
MvnI 425 CGCG CG^CG
RcaI 510 TCATGA T^CATGA
Sau96I 404 GGTCC G^G[ACGT]CC
SfcI 980 CTGTAG C^T[GA][CT]AG
SspBI 107 TGTACA T^GTACA
Tru9I 713 TTAA T^TAA
XcmI 437 CCAGCATTGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 3 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AfaI 108 GTAC GT^AC
128 GTAC
BbsI 307 GTCTTC GAAGAC(2/6)
925 GTCTTC
BbvI 411 GCAGC GCAGC(8/12)
469 GCTGC
BclI 8 TGATCA T^GATCA
29 TGATCA
BpuAI 307 GTCTTC GAAGAC(2/6)
925 GTCTTC
BsaJI 447 CCTGGG C^C[ACGT][ACGT]GG
792 CCAGGG
BsiYI 422 CCCCGCGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
423 CCCGCGCTGGG
BslI 422 CCCCGCGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
423 CCCGCGCTGGG
BsmAI 502 GAGAC GTCTC(1/5)
324 GTCTC
BssKI 447 CCTGG ^CC[ACGT]GG
792 CCAGG
BstNI 447 CCTGG CC^[AT]GG
792 CCAGG
EaeI 251 TGGCCA [CT]^GGCC[GA]
359 CGGCCA
EcoRII 447 CCTGG ^CC[AT]GG
792 CCAGG
FbaI 8 TGATCA T^GATCA
29 TGATCA
HincII 834 GTTGAC GT[CT]^[GA]AC
957 GTCAAC
HindII 834 GTTGAC GT[CT]^[GA]AC
957 GTCAAC
MvaI 447 CCTGG CC^[AT]GG
792 CCAGG
NlaIII 511 CATG CATG^
604 CATG
PleI 452 GAGTC GAGTC(4/5)
955 GAGTC
RsaI 108 GTAC GT^AC
128 GTAC
ScrFI 447 CCTGG CC^[ACGT]GG
792 CCAGG
TseI 411 GCAGC G^C[AT]GC
469 GCTGC
Tsp45I 529 GTGAC ^GT[GC]AC
819 GTCAC
Tsp509I 123 AATT ^AATT
913 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 22 enzymes) #
#########################################
AciI BsrI BstF5I Cac8I DdeI
DpnII Fnu4HI FokI HaeIII HinfI
HphI ItaI MaeIII MboI MboII
MnlI NdeII NlaIV Sau3AI SfaNI
TfiI TspRI