Restriction map for
pFN21AB8541
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# NO CUT ENZYMES ( 103 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI Aor51HI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AvrII BbrPI BbsI
BciVI BfrI BglII BlnI BpuAI
BseAI BsiEI BsiWI Bsp1407I BspDI
BspEI BspHI BsrDI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstZ17I ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoO65I EcoRI EcoT22I FseI
HincII HindII HindIII HpaI KpnI
MfeI MluI MroI MseI MunI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RcaI RsrII SacI SalI SapI
SbfI ScaI SfiI SfuI SgrAI
SnaBI SpeI Sse8387I SspBI SspI
StuI SwaI Tru9I Tsp509I XbaI
XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AcyI 5 GGCGCC G[GA]^CG[CT]C
AfaI 1881 GTAC GT^AC
AflIII 1493 ACATGT A^C[GA][CT]GT
AviII 1701 TGCGCA TGC^GCA
BamHI 34 GGATCC G^GATCC
BbeI 5 GGCGCC GGCGC^C
BclI 1428 TGATCA T^GATCA
BsaAI 2497 CACGTA [CT]AC^GT[GA]
BsaBI 2805 GATTGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 5 GGCGCC G[GA]^CG[CT]C
BsaWI 1944 ACCGGA [AT]^CCGG[AT]
BsmBI 2587 GAGACG CGTCTC(1/5)
BsmI 2910 GAATGC GAATGC(1/-1)
BspLU11I 1493 ACATGT A^CATGT
EarI 727 CTCTTC CTCTTC(1/4)
EcoRV 1150 GATATC GAT^ATC
FbaI 1428 TGATCA T^GATCA
FspI 1701 TGCGCA TGC^GCA
Hin1I 5 GGCGCC G[GA]^CG[CT]C
KasI 5 GGCGCC G^GCGCC
Ksp632I 727 CTCTTC CTCTTC(1/4)
KspI 10 CCGCGG CCGC^GG
MamI 2805 GATTGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 5 GGCGCC GG^CGCC
NcoI 1860 CCATGG C^CATGG
PshAI 1320 GACACCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsaI 1881 GTAC GT^AC
SacII 10 CCGCGG CCGC^GG
SfoI 5 GGCGCC GGC^GCC
SmaI 84 CCCGGG CCC^GGG
SmlI 2920 CTCAAG C^T[CT][GA]AG
SphI 1806 GCATGC GCATG^C
TaqI 2765 TCGA T^CGA
TfiI 2082 GATTC G^A[AT]TC
TthHB8I 2765 TCGA T^CGA
XmaCI 84 CCCGGG C^CCGGG
XmaI 84 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 29 enzymes) #
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Alw44I 1489 GTGCAC G^TGCAC
2719 GTGCAC
AocI 1499 CCTCAGG CC^T[ACGT]AGG
1849 CCTGAGG
ApaI 1856 GGGCCC GGGCC^C
2352 GGGCCC
ApaLI 1489 GTGCAC G^TGCAC
2719 GTGCAC
AspI 398 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2767 GACATGGTC
AvaI 28 CTCGGG C^[CT]CG[GA]G
84 CCCGGG
BbvCI 1385 CCTCAGC CCTCAGC(-5/-2)
2616 CCTCAGC
BcgI 2316 GCATGACCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2408 GCACCGTCATCG
BfaI 949 CTAG C^TAG
1333 CTAG
BglI 83 GCCCGGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1346 GCCCACTGGGC
BsoBI 28 CTCGGG C^[CT]CG[GA]G
84 CCCGGG
BstXI 633 CCACCAGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2841 CCAGCGGCCTGG
BstYI 34 GGATCC [GA]^GATC[CT]
1472 AGATCC
Bsu36I 1499 CCTCAGG CC^T[ACGT]AGG
1849 CCTGAGG
Eco81I 1499 CCTCAGG CC^T[ACGT]AGG
1849 CCTGAGG
EcoNI 702 CCTGCCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2124 CCTTGCCAAGG
MaeI 949 CTAG C^TAG
1333 CTAG
MaeII 2498 ACGT A^CGT
2573 ACGT
MflI 34 GGATCC [GA]^GATC[CT]
1472 AGATCC
NaeI 2043 GCCGGC GCC^GGC
2625 GCCGGC
NgoMIV 2043 GCCGGC G^CCGGC
2625 GCCGGC
PflFI 398 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2767 GACATGGTC
PflMI 1899 CCAGCACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2129 CCAAGGAGTGG
PleI 823 GACTC GAGTC(4/5)
919 GACTC
PpuMI 785 GGGACCT [GA]G^G[AT]CC[CT]
1886 AGGACCT
SexAI 399 ACCTGGT A^CC[AT]GGT
2513 ACCTGGT
Tth111I 398 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2767 GACATGGTC
Van91I 1899 CCAGCACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2129 CCAAGGAGTGG
XhoII 34 GGATCC [GA]^GATC[CT]
1472 AGATCC
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# 3 OR MORE CUT ENZYMES ( 89 enzymes) #
#########################################
AccII AciI AluI AlwI AlwNI
AspHI AvaII BalI BanI BanII
BbvI BcnI BlpI BmyI BpmI
Bpu1102I BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspMI BsrBI BsrFI BsrI
BssKI BstAPI BstF5I BstNI BstUI
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI MaeIII MboI MboII MluNI
MnlI MscI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PstI PvuII
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TseI Tsp45I TspRI