Restriction map for
pFN21AE2116
##################################
# NO CUT ENZYMES ( 113 enzymes) #
##################################
AatII Acc65I AccII AciI AclI
AcyI AflII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AscI Asp718I AspEI AspI AviII
AvrII BalI BamHI BbeI BbrPI
BcgI BciVI BcnI BfrI BglI
BlnI BpmI BsaHI BsaI BseRI
BsiEI BsiWI BsmBI BspDI BspHI
BspMI BsrBI BsrFI BssHII BssSI
BstUI BstXI Bsu36I Cfr10I ClaI
CpoI DraIII DrdI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoT22I FauI FseI FspI HgaI
Hin1I HindIII KasI KpnI KspI
MfeI MluI MluNI MscI MslI
MunI MvnI NaeI NarI NciI
NgoMIV NotI NruI NsiI PacI
PflFI PinAI PmaCI PmeI PmlI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SbfI SexAI SfiI
SfoI SgrAI SmaI SnaBI SpeI
Sse8387I SspI StuI SwaI Tth111I
XcmI XmaCI XmaI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AflIII 1435 ACATGT A^C[GA][CT]GT
AspHI 2441 GAGCAC G[AT]GC[AT]^C
BanI 304 GGTGCC G^G[CT][GA]CC
BanII 1195 GAGCCC G[GA]GC[CT]^C
BbvCI 478 GCTGAGG CCTCAGC(-5/-2)
BglII 2055 AGATCT A^GATCT
BlpI 278 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 278 GCTCAGC GC^T[ACGT]AGC
BsaAI 2598 CACGTA [CT]AC^GT[GA]
BsaBI 250 GATCCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiHKAI 2441 GAGCAC G[AT]GC[AT]^C
BsmI 1452 GAATGC GAATGC(1/-1)
BspLU11I 1435 ACATGT A^CATGT
BsrDI 1058 GCAATG GCAATG(2/0)
BstAPI 589 GCATGTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1113 TTCGAA TT^CGAA
CelII 278 GCTCAGC GC^T[ACGT]AGC
CfoI 350 GCGC GCG^C
DsaI 1901 CCATGG C^C[GA][CT]GG
EcoNI 2446 CCTGTGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1493 GAATTC G^AATTC
EcoRV 2043 GATATC GAT^ATC
HaeII 349 GGCGCT [GA]GCGC^[CT]
HhaI 350 GCGC GCG^C
HinP1I 350 GCGC G^CGC
HincII 117 GTTAAC GT[CT]^[GA]AC
HindII 117 GTTAAC GT[CT]^[GA]AC
HpaI 117 GTTAAC GTT^AAC
MamI 250 GATCCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NcoI 1901 CCATGG C^CATGG
NdeI 2212 CATATG CA^TATG
NheI 61 GCTAGC G^CTAGC
NspV 1113 TTCGAA TT^CGAA
PflMI 1753 CCAGATATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1263 AGGTCCT [GA]G^G[AT]CC[CT]
PshAI 977 GACTGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 1643 CTGCAG CTGCA^G
SapI 1192 GAAGAGC GCTCTTC(1/4)
SfuI 1113 TTCGAA TT^CGAA
SphI 2618 GCATGC GCATG^C
Van91I 1753 CCAGATATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccIII 500 TCCGGA T^CCGGA
2547 TCCGGA
AlwI 250 GATCC GGATC(4/5)
838 GATCC
AseI 232 ATTAAT AT^TAAT
1348 ATTAAT
AsnI 232 ATTAAT AT^TAAT
1348 ATTAAT
AvaII 1264 GGTCC G^G[AT]CC
1275 GGACC
BbsI 556 GAAGAC GAAGAC(2/6)
613 GAAGAC
BclI 971 TGATCA T^GATCA
2030 TGATCA
BpuAI 556 GAAGAC GAAGAC(2/6)
613 GAAGAC
BsaWI 500 TCCGGA [AT]^CCGG[AT]
2547 TCCGGA
BseAI 500 TCCGGA T^CCGGA
2547 TCCGGA
BsgI 10 CTGCAC GTGCAG(16/14)
144 CTGCAC
BsmFI 2345 GGGAC GGGAC(10/14)
1439 GTCCC
Bsp1407I 1502 TGTACA T^GTACA
1890 TGTACA
BspEI 500 TCCGGA T^CCGGA
2547 TCCGGA
BsrGI 1502 TGTACA T^GTACA
1890 TGTACA
Bst1107I 826 GTATAC GTA^TAC
1880 GTATAC
BstYI 837 AGATCC [GA]^GATC[CT]
2055 AGATCT
BstZ17I 826 GTATAC GTA^TAC
1880 GTATAC
DraI 364 TTTAAA TTT^AAA
1983 TTTAAA
DraII 681 AGGCCCT [GA]G^G[ACGT]CC[CT]
1263 AGGTCCT
EcoO109I 681 AGGCCCT [GA]G^G[ACGT]CC[CT]
1263 AGGTCCT
FbaI 971 TGATCA T^GATCA
2030 TGATCA
MflI 837 AGATCC [GA]^GATC[CT]
2055 AGATCT
MroI 500 TCCGGA T^CCGGA
2547 TCCGGA
MspA1I 80 CAGCTG C[AC]G^C[GT]G
1726 CAGCTG
NlaIV 304 GGTGCC GG[ACGT]^[ACGT]CC
1275 GGACCC
PshBI 232 ATTAAT AT^TAAT
1348 ATTAAT
PvuII 80 CAGCTG CAG^CTG
1726 CAGCTG
ScaI 1851 AGTACT AGT^ACT
2240 AGTACT
SspBI 1502 TGTACA T^GTACA
1890 TGTACA
XbaI 1371 TCTAGA T^CTAGA
2292 TCTAGA
XhoII 837 AGATCC [GA]^GATC[CT]
2055 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 72 enzymes) #
#########################################
AccI AcsI AfaI AluI ApoI
Asp700I AvaI BbvI BfaI BmyI
BsaJI BsiYI BslI BsmAI BsoBI
Bsp1286I BsrI BssKI BstEII BstF5I
BstNI BstPI Cac8I Cfr13I DdeI
DpnII EarI EcoO65I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MnlI
MseI MspI MvaI MwoI NdeII
NlaIII NspI PaeR7I PleI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoI XmnI