Restriction map for
pFN21AE4170
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# NO CUT ENZYMES ( 118 enzymes) #
##################################
AatII Acc65I AccI AccII AciI
AclI AhdI Alw44I AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AviII AvrII BalI BanII BbrPI
BbvCI BcgI BclI BglI BlnI
BsaAI BsaBI BsiEI BsiHKAI BsiWI
BsmBI BspDI BsrBI BssHII BssSI
Bst1107I BstEII BstPI BstUI BstXI
BstZ17I Bsu36I ClaI CpoI DraI
DraII DrdI DsaI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRV EcoT22I
FauI FbaI FseI FspI KpnI
KspI MaeII MamI MfeI MluI
MluNI MscI MunI MvnI NaeI
NcoI NdeI NgoMIV NotI NruI
NsiI PacI PaeR7I PflFI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RsrII SacI
SacII SalI SapI SbfI ScaI
SfiI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SwaI Tth111I XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 64 enzymes) #
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AccIII 1442 TCCGGA T^CCGGA
AflII 1728 CTTAAG C^TTAAG
AflIII 420 ACATGT A^C[GA][CT]GT
AgeI 6 ACCGGT A^CCGGT
AlwNI 1684 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AvaI 811 CTCGGG C^[CT]CG[GA]G
AvaII 1445 GGACC G^G[AT]CC
BamHI 1120 GGATCC G^GATCC
BbeI 59 GGCGCC GGCGC^C
BbsI 1393 GAAGAC GAAGAC(2/6)
BciVI 405 GTATCC GTATCC(6/5)
BfrI 1728 CTTAAG C^TTAAG
BlpI 1329 GCTCAGC GC^T[ACGT]AGC
BmyI 1542 GTGCCC G[AGT]GC[ACT]^C
BpmI 1519 CTGGAG CTGGAG(16/14)
Bpu1102I 1329 GCTCAGC GC^T[ACGT]AGC
BpuAI 1393 GAAGAC GAAGAC(2/6)
BsaI 534 GAGACC GGTCTC(1/5)
BseAI 1442 TCCGGA T^CCGGA
BsgI 1029 CTGCAC GTGCAG(16/14)
BsmFI 301 GGGAC GGGAC(10/14)
BsoBI 811 CTCGGG C^[CT]CG[GA]G
Bsp1286I 1542 GTGCCC G[AGT]GC[ACT]^C
Bsp1407I 417 TGTACA T^GTACA
BspEI 1442 TCCGGA T^CCGGA
BspHI 1385 TCATGA T^CATGA
BspLU11I 420 ACATGT A^CATGT
BspMI 1100 GCAGGT ACCTGC(4/8)
BsrFI 6 ACCGGT [GA]^CCGG[CT]
BsrGI 417 TGTACA T^GTACA
BstAPI 1562 GCACCGGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 290 TTCGAA TT^CGAA
CelII 1329 GCTCAGC GC^T[ACGT]AGC
CfoI 60 GCGC GCG^C
Cfr10I 6 ACCGGT [GA]^CCGG[CT]
DraIII 1563 CACCGGGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 635 CTCTTC CTCTTC(1/4)
EcoRI 458 GAATTC G^AATTC
EcoT14I 1533 CCTTGG C^C[AT][AT]GG
HaeII 59 GGCGCC [GA]GCGC^[CT]
HgaI 348 GACGC GACGC(5/10)
HhaI 60 GCGC GCG^C
HinP1I 60 GCGC G^CGC
HincII 21 GTTAAC GT[CT]^[GA]AC
HindII 21 GTTAAC GT[CT]^[GA]AC
HpaI 21 GTTAAC GTT^AAC
KasI 59 GGCGCC G^GCGCC
Ksp632I 635 CTCTTC CTCTTC(1/4)
MroI 1442 TCCGGA T^CCGGA
MslI 937 CATTGCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 59 GGCGCC GG^CGCC
NheI 1411 GCTAGC G^CTAGC
NspV 290 TTCGAA TT^CGAA
PinAI 6 ACCGGT A^CCGGT
PstI 1552 CTGCAG CTGCA^G
RcaI 1385 TCATGA T^CATGA
SexAI 1675 ACCAGGT A^CC[AT]GGT
SfoI 59 GGCGCC GGC^GCC
SfuI 290 TTCGAA TT^CGAA
SspBI 417 TGTACA T^GTACA
StuI 1211 AGGCCT AGG^CCT
StyI 1533 CCTTGG C^C[AT][AT]GG
XbaI 426 TCTAGA T^CTAGA
XcmI 164 CCAAAGAAGTAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AcyI 59 GGCGCC G[GA]^CG[CT]C
348 GACGCC
BanI 59 GGCGCC G^G[CT][GA]CC
1541 GGTGCC
BcnI 442 CCCGG CC^[GC]GG
1565 CCGGG
BglII 1630 AGATCT A^GATCT
1755 AGATCT
BsaHI 59 GGCGCC G[GA]^CG[CT]C
348 GACGCC
BsaWI 6 ACCGGT [AT]^CCGG[AT]
1442 TCCGGA
BsmAI 534 GAGAC GTCTC(1/5)
1023 GTCTC
BsmI 549 GAATGC GAATGC(1/-1)
1818 GAATGC
BsrDI 937 CATTGC GCAATG(2/0)
1263 CATTGC
Cfr13I 820 GGGCC G^G[ACGT]CC
1445 GGACC
HaeIII 821 GGCC GG^CC
1212 GGCC
Hin1I 59 GGCGCC G[GA]^CG[CT]C
348 GACGCC
HindIII 1250 AAGCTT A^AGCTT
1824 AAGCTT
NciI 442 CCCGG CC^[GC]GG
1565 CCGGG
NspI 420 ACATGT [GA]CATG^[CT]
1663 GCATGT
PflMI 256 CCAAGTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
823 CCAAATAATGG
Sau96I 820 GGGCC G^G[ACGT]CC
1445 GGACC
SspI 644 AATATT AAT^ATT
1471 AATATT
TaqI 291 TCGA T^CGA
1302 TCGA
TthHB8I 291 TCGA T^CGA
1302 TCGA
Van91I 256 CCAAGTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
823 CCAAATAATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
BbvI BfaI BsaJI BseRI BsiYI
BslI BsrI BssKI BstF5I BstNI
BstYI Cac8I DdeI DpnII EcoRII
Fnu4HI FokI HapII HinfI HpaII
HphI ItaI MaeI MaeIII MboI
MboII MflI MnlI MseI MspA1I
MspI MvaI MwoI NdeII NlaIII
NlaIV PvuII RsaI Sau3AI ScrFI
SfaNI SfcI SmlI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI XhoII