##################################
# NO CUT ENZYMES (  198 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AcyI        AflII       AgeI        AhdI        
Alw44I      AocI        ApaI        ApaLI       ApoI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspHI       AspI        AvaI        AviII       
AvrII       BalI        BamHI       BanI        BbeI        
BbrPI       BbsI        BbvCI       BcgI        BciVI       
BclI        BcnI        BfrI        BglI        BglII       
BlnI        BlpI        Bpu1102I    BpuAI       BsaBI       
BsaHI       BsaWI       BseAI       BsgI        BsiEI       
BsiHKAI     BsiWI       BsiYI       BslI        BsmBI       
BsmFI       BsmI        BsoBI       Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BspMI       BsrBI       
BsrDI       BsrFI       BsrGI       BssHII      BssSI       
Bst1107I    BstBI       BstEII      BstF5I      BstPI       
BstXI       BstYI       BstZ17I     Bsu36I      CelII       
Cfr10I      ClaI        CpoI        DraI        DraII       
DraIII      DsaI        EaeI        EagI        Eam1105I    
EarI        EclXI       Eco52I      Eco81I      EcoNI       
EcoO109I    EcoO65I     EcoRI       EcoRV       EcoT14I     
EcoT22I     FauI        FbaI        FokI        FseI        
FspI        HaeIII      HapII       HgaI        Hin1I       
HincII      HindII      HinfI       HpaI        HpaII       
HphI        KasI        KpnI        Ksp632I     KspI        
MamI        MboII       MfeI        MflI        MluI        
MluNI       MroI        MscI        MseI        MslI        
MspI        MunI        NaeI        NarI        NciI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PleI        
PmaCI       PmeI        PmlI        PpuMI       PshBI       
Psp1406I    PstI        PvuI        RcaI        RsrII       
SacI        SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfaNI       SfcI        SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        StyI        SwaI        TaqI        
TfiI        Tru9I       Tsp509I     Tth111I     TthHB8I     
Van91I      XbaI        XcmI        XhoI        XhoII       
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   43 enzymes) #
###################################

AccII         117  CGCG                 CG^CG
AfaI          252  GTAC                 GT^AC
AflIII        254  ACGTGT               A^C[GA][CT]GT
AlwI          226  GATCC                GGATC(4/5)
AlwNI         165  CAGCCTCTG            CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI        60  AGCGCT               AGC^GCT
AvaII         243  GGTCC                G^G[AT]CC
BanII          78  GGGCTC               G[GA]GC[CT]^C
BfaI           37  CTAG                 C^TAG
BmyI           78  GGGCTC               G[AGT]GC[ACT]^C
BpmI           56  CTCCAG               CTGGAG(16/14)
BsaAI         253  TACGTG               [CT]AC^GT[GA]
BsaJI         239  CCTGGG               C^C[ACGT][ACGT]GG
BseRI          28  CTCCTC               GAGGAG(10/8)
Bsp1286I       78  GGGCTC               G[AGT]GC[ACT]^C
BsrI          230  CCAGT                ACTGG(1/-1)
BstAPI        164  GCAGCCTCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI         117  CGCG                 CG^CG
CfoI           61  GCGC                 GCG^C
Cfr13I        243  GGTCC                G^G[ACGT]CC
DpnII         226  GATC                 ^GATC
DrdI           15  GACTGTCCTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III       60  AGCGCT               AGC^GCT
HaeII          60  AGCGCT               [GA]GCGC^[CT]
HhaI           61  GCGC                 GCG^C
HinP1I         61  GCGC                 G^CGC
HindIII       124  AAGCTT               A^AGCTT
MaeI           37  CTAG                 C^TAG
MaeII         254  ACGT                 A^CGT
MaeIII         13  GTGAC                ^GT[ACGT]AC
MboI          226  GATC                 ^GATC
MspA1I        182  CAGCTG               C[AC]G^C[GT]G
MvnI          117  CGCG                 CG^CG
NdeII         226  GATC                 ^GATC
NlaIII         33  CATG                 CATG^
NlaIV         242  GGGTCC               GG[ACGT]^[ACGT]CC
PshAI         237  GACCTGGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII         182  CAGCTG               CAG^CTG
RsaI          252  GTAC                 GT^AC
Sau3AI        226  GATC                 ^GATC
Sau96I        243  GGTCC                G^G[ACGT]CC
Tsp45I         13  GTGAC                ^GT[GC]AC
TspRI         231  CAGTG                CA[GC]TG[ACGT][ACGT]^


###################################
# TWO CUT ENZYMES (    4 enzymes) #
###################################

AciI           95  CCGC                 CCGC(-3/-1)
              116  CCGC                
BsaI          157  GAGACC               GGTCTC(1/5)
              235  GAGACC              
DdeI           66  CTCAG                C^T[ACGT]AG
               81  CTCAG               
MwoI           61  GCGCTCTCAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              164  GCAGCCTCTGC         


#########################################
# 3 OR MORE CUT ENZYMES (   13 enzymes) #
#########################################

AluI        BbvI        BsmAI       BssKI       BstNI       
Cac8I       EcoRII      Fnu4HI      ItaI        MnlI        
MvaI        ScrFI       TseI