Restriction map for
pFN21AE3562
##################################
# NO CUT ENZYMES ( 149 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI AhdI Alw44I
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AvaI
AviII AvrII BamHI BanI BbeI
BbrPI BcgI BciVI BcnI BfrI
BglI BglII BlnI BlpI BpmI
Bpu1102I BsaBI BsaHI BsaWI BseAI
BseRI BsiEI BsiWI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstEII BstPI BstUI BstXI
CelII CfoI Cfr10I ClaI CpoI
DraI DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoO65I EcoRI EcoRV
EcoT22I FauI FseI FspI HaeII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HpaI HpaII KasI
KpnI KspI MamI MfeI MluI
MroI MspA1I MspI MunI MvnI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
PacI PaeR7I PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacII SalI SbfI ScaI
SexAI SfiI SfoI SgrAI SmaI
SnaBI SpeI Sse8387I SspBI SspI
StuI SwaI Van91I XbaI XcmI
XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AciI 522 GCGG CCGC(-3/-1)
AcsI 516 AAATTT [GA]^AATT[CT]
AflIII 950 ACATGT A^C[GA][CT]GT
AlwNI 68 CAGACCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 516 AAATTT [GA]^AATT[CT]
AspI 70 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 818 GGACC G^G[AT]CC
BalI 779 TGGCCA TGG^CCA
BbsI 910 GAAGAC GAAGAC(2/6)
BclI 533 TGATCA T^GATCA
BpuAI 910 GAAGAC GAAGAC(2/6)
BsaAI 589 CACGTA [CT]AC^GT[GA]
BsaI 481 GAGACC GGTCTC(1/5)
BsmBI 746 GAGACG CGTCTC(1/5)
BspLU11I 950 ACATGT A^CATGT
BstBI 907 TTCGAA TT^CGAA
DraII 1204 AGGCCCC [GA]G^G[ACGT]CC[CT]
DraIII 15 CACGCAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1338 CCACGG C^C[GA][CT]GG
EaeI 779 TGGCCA [CT]^GGCC[GA]
EcoNI 729 CCTGCCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 1204 AGGCCCC [GA]G^G[ACGT]CC[CT]
FbaI 533 TGATCA T^GATCA
HindIII 898 AAGCTT A^AGCTT
MluNI 779 TGGCCA TGG^CCA
MscI 779 TGGCCA TGG^CCA
MslI 825 CACTGGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NspV 907 TTCGAA TT^CGAA
PflFI 70 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
SapI 577 GAAGAGC GCTCTTC(1/4)
SfaNI 1304 GCATC GCATC(5/9)
SfuI 907 TTCGAA TT^CGAA
SmlI 634 CTCAAG C^T[CT][GA]AG
SphI 360 GCATGC GCATG^C
Tsp45I 76 GTCAC ^GT[GC]AC
Tth111I 70 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 38 enzymes) #
###################################
AccI 668 GTATAC GT^[AC][GT]AC
978 GTATAC
AfaI 186 GTAC GT^AC
971 GTAC
AspHI 34 GAGCTC G[AT]GC[AT]^C
995 GAGCTC
BanII 34 GAGCTC G[GA]GC[CT]^C
995 GAGCTC
BbvCI 787 CCTCAGC CCTCAGC(-5/-2)
1005 CCTCAGC
BbvI 932 GCAGC GCAGC(8/12)
152 GCTGC
BfaI 650 CTAG C^TAG
1219 CTAG
BsiHKAI 34 GAGCTC G[AT]GC[AT]^C
995 GAGCTC
BsmFI 107 GTCCC GGGAC(10/14)
241 GTCCC
BspMI 40 ACCTGC ACCTGC(4/8)
728 ACCTGC
Bst1107I 668 GTATAC GTA^TAC
978 GTATAC
BstF5I 1035 GGATG GGATG(2/0)
1149 GGATG
BstYI 1001 AGATCC [GA]^GATC[CT]
1342 GGATCT
BstZ17I 668 GTATAC GTA^TAC
978 GTATAC
Cfr13I 818 GGACC G^G[ACGT]CC
1205 GGCCC
EarI 1165 CTCTTC CTCTTC(1/4)
577 GAAGAG
Fnu4HI 152 GCTGC GC^[ACGT]GC
932 GCAGC
FokI 1035 GGATG GGATG(9/13)
1149 GGATG
HaeIII 780 GGCC GG^CC
1205 GGCC
ItaI 152 GCTGC GC^[ACGT]GC
932 GCAGC
Ksp632I 1165 CTCTTC CTCTTC(1/4)
577 GAAGAG
MaeI 650 CTAG C^TAG
1219 CTAG
MaeII 590 ACGT A^CGT
749 ACGT
MaeIII 76 GTCAC ^GT[ACGT]AC
1185 GTTAC
MflI 1001 AGATCC [GA]^GATC[CT]
1342 GGATCT
MseI 845 TTAA T^TAA
1262 TTAA
NlaIV 323 GGAGCC GG[ACGT]^[ACGT]CC
1205 GGCCCC
PleI 1045 GACTC GAGTC(4/5)
1111 GACTC
RsaI 186 GTAC GT^AC
971 GTAC
SacI 34 GAGCTC GAGCT^C
995 GAGCTC
Sau96I 818 GGACC G^G[ACGT]CC
1205 GGCCC
SfcI 276 CTGTAG C^T[GA][CT]AG
405 CTATAG
TaqI 46 TCGA T^CGA
908 TCGA
TfiI 235 GAATC G^A[AT]TC
423 GATTC
Tru9I 845 TTAA T^TAA
1262 TTAA
TseI 152 GCTGC G^C[AT]GC
932 GCAGC
TthHB8I 46 TCGA T^CGA
908 TCGA
XhoII 1001 AGATCC [GA]^GATC[CT]
1342 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 35 enzymes) #
#########################################
AluI AlwI AocI BmyI BsaJI
BsgI BsiYI BslI BsmAI Bsp1286I
BsrI BssKI BstNI Bsu36I Cac8I
DdeI DpnII Eco81I EcoRII EcoT14I
HinfI HphI MboI MboII MnlI
MvaI MwoI NdeII NlaIII NspI
Sau3AI ScrFI StyI Tsp509I TspRI