Restriction map for
pFN21AE3723
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# NO CUT ENZYMES ( 100 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AgeI AlwNI ApaI AscI
AseI AsnI Asp718I AspI AviII
BbeI BbvCI BcgI BciVI BclI
BglII BlpI Bpu1102I BsaBI BsaHI
BsaI BsaWI BseAI BsiEI BsiWI
BsmI Bsp1407I BspEI BsrGI BssHII
Bst1107I BstBI BstEII BstPI BstXI
BstZ17I CelII CpoI DraI DrdI
EagI EclXI Eco52I EcoO65I EcoT22I
FbaI FseI FspI Hin1I HincII
HindII HpaI KasI KpnI MamI
MfeI MluI MroI MunI NarI
NdeI NheI NotI NruI NsiI
NspV PacI PflFI PflMI PinAI
PmeI PshAI PshBI Psp1406I PstI
PvuI RsrII SalI SapI SbfI
SexAI SfcI SfiI SfoI SfuI
SgrAI SnaBI SpeI Sse8387I SspBI
SspI SwaI Tth111I Van91I XbaI
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# ONE CUT ENZYMES ( 34 enzymes) #
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AflII 841 CTTAAG C^TTAAG
AflIII 815 ACATGT A^C[GA][CT]GT
AhdI 1315 GACCGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1609 GTGCAC G^TGCAC
AocI 1306 CCTGAGG CC^T[ACGT]AGG
Aor51HI 7 AGCGCT AGC^GCT
ApaLI 1609 GTGCAC G^TGCAC
AspEI 1315 GACCGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 705 CCTAGG C^CTAGG
BamHI 736 GGATCC G^GATCC
BfrI 841 CTTAAG C^TTAAG
BglI 1534 GCCTCCACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 705 CCTAGG C^CTAGG
BsmBI 1966 GAGACG CGTCTC(1/5)
BspDI 902 ATCGAT AT^CGAT
BspHI 893 TCATGA T^CATGA
BspLU11I 815 ACATGT A^CATGT
BstAPI 1821 GCAGAATTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 1306 CCTGAGG CC^T[ACGT]AGG
ClaI 902 ATCGAT AT^CGAT
DraIII 1981 CACGTGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 1315 GACCGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 7 AGCGCT AGC^GCT
Eco81I 1306 CCTGAGG CC^T[ACGT]AGG
EcoNI 1476 CCTCGGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 2271 GATATC GAT^ATC
HindIII 2329 AAGCTT A^AGCTT
NaeI 1874 GCCGGC GCC^GGC
NgoMIV 1874 GCCGGC G^CCGGC
PaeR7I 455 CTCGAG C^TCGAG
RcaI 893 TCATGA T^CATGA
ScaI 491 AGTACT AGT^ACT
SphI 2174 GCATGC GCATG^C
XhoI 455 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 29 enzymes) #
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Asp700I 285 GAAACTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2017 GAAGAATTTC
BalI 1064 TGGCCA TGG^CCA
1860 TGGCCA
BbrPI 1981 CACGTG CAC^GTG
2055 CACGTG
BsgI 487 GTGCAG GTGCAG(16/14)
2206 GTGCAG
BspMI 539 ACCTGC ACCTGC(4/8)
836 ACCTGC
BsrBI 2040 CCGCTC CCGCTC(-3/-3)
1465 GAGCGG
BsrDI 2301 CATTGC GCAATG(2/0)
590 GCAATG
BsrFI 158 ACCGGC [GA]^CCGG[CT]
1874 GCCGGC
BsrI 223 ACTGG ACTGG(1/-1)
726 ACTGG
BssSI 2073 CACGAG CACGAG(-5/-1)
2194 CACGAG
Cfr10I 158 ACCGGC [GA]^CCGG[CT]
1874 GCCGGC
EarI 658 CTCTTC CTCTTC(1/4)
1018 GAAGAG
EcoRI 595 GAATTC G^AATTC
1449 GAATTC
Ksp632I 658 CTCTTC CTCTTC(1/4)
1018 GAAGAG
MluNI 1064 TGGCCA TGG^CCA
1860 TGGCCA
MscI 1064 TGGCCA TGG^CCA
1860 TGGCCA
MslI 922 CACGAAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2280 CACCTCCATG
PmaCI 1981 CACGTG CAC^GTG
2055 CACGTG
PmlI 1981 CACGTG CAC^GTG
2055 CACGTG
PpuMI 971 AGGACCC [GA]G^G[AT]CC[CT]
1525 GGGTCCC
PvuII 1139 CAGCTG CAG^CTG
2133 CAGCTG
SacI 1092 GAGCTC GAGCT^C
2076 GAGCTC
SmaI 1529 CCCGGG CCC^GGG
1760 CCCGGG
StuI 1484 AGGCCT AGG^CCT
1622 AGGCCT
TfiI 102 GATTC G^A[AT]TC
321 GAATC
XcmI 194 CCAAGGTGAAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2072 CCACGAGCTCCATGG
XmaCI 1529 CCCGGG C^CCGGG
1760 CCCGGG
XmaI 1529 CCCGGG C^CCGGG
1760 CCCGGG
XmnI 285 GAAACTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2017 GAAGAATTTC
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# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApoI AspHI AvaI AvaII
BanI BanII BbsI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaAI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BssKI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI KspI MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PleI RsaI
SacII Sau3AI Sau96I ScrFI SfaNI
SmlI StyI TaqI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII