Restriction map for
pFN21AE2192
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# NO CUT ENZYMES ( 140 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflIII AgeI AhdI Aor51HI
ApaI AscI AseI AsnI Asp718I
AspEI AspI AvaI AviII AvrII
BalI BamHI BanI BanII BbeI
BbrPI BclI BcnI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BsaWI BseAI BsgI BsiEI
BsiWI BsmBI BsoBI Bsp1407I BspDI
BspEI BspLU11I BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstZ17I Cac8I CelII Cfr10I
ClaI CpoI DraIII DrdI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoRI EcoRV EcoT22I FbaI
FseI FspI HaeIII HapII HgaI
Hin1I HpaII KasI KpnI MamI
MluI MluNI MroI MscI MspI
NaeI NarI NciI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RsrII SacI SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TaqI Tth111I TthHB8I Van91I
XbaI XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
AccII 40 CGCG CG^CG
AflII 406 CTTAAG C^TTAAG
Alw44I 1648 GTGCAC G^TGCAC
AlwNI 812 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 1648 GTGCAC G^TGCAC
Asp700I 1170 GAAAGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 1648 GTGCAC G[AT]GC[AT]^C
BbvCI 1444 GCTGAGG CCTCAGC(-5/-2)
BcgI 541 CGAAGCAAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 406 CTTAAG C^TTAAG
BseRI 1301 CTCCTC GAGGAG(10/8)
BsiHKAI 1648 GTGCAC G[AT]GC[AT]^C
BsiYI 1460 CCGCAGACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 1460 CCGCAGACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmI 241 GAATGC GAATGC(1/-1)
BspMI 1644 GCAGGT ACCTGC(4/8)
BssKI 1166 CCAGG ^CC[ACGT]GG
BstEII 1307 GGTCACC G^GT[ACGT]ACC
BstNI 1166 CCAGG CC^[AT]GG
BstPI 1307 GGTCACC G^GT[ACGT]ACC
BstUI 40 CGCG CG^CG
BstXI 259 CCATGTGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 355 AGATCC [GA]^GATC[CT]
CfoI 72 GCGC GCG^C
DraI 1125 TTTAAA TTT^AAA
DraII 535 AGGACCC [GA]G^G[ACGT]CC[CT]
EarI 753 GAAGAG CTCTTC(1/4)
EcoO109I 535 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoO65I 1307 GGTCACC G^GT[ACGT]ACC
EcoRII 1166 CCAGG ^CC[AT]GG
EcoT14I 1348 CCATGG C^C[AT][AT]GG
FauI 87 CCCGC CCCGC(4/6)
HaeII 71 AGCGCC [GA]GCGC^[CT]
HhaI 72 GCGC GCG^C
HinP1I 72 GCGC G^CGC
HincII 667 GTTAAC GT[CT]^[GA]AC
HindII 667 GTTAAC GT[CT]^[GA]AC
HindIII 1354 AAGCTT A^AGCTT
HpaI 667 GTTAAC GTT^AAC
Ksp632I 753 GAAGAG CTCTTC(1/4)
KspI 39 CCGCGG CCGC^GG
MfeI 1574 CAATTG C^AATTG
MflI 355 AGATCC [GA]^GATC[CT]
MunI 1574 CAATTG C^AATTG
MvaI 1166 CCAGG CC^[AT]GG
MvnI 40 CGCG CG^CG
NcoI 1348 CCATGG C^CATGG
NspI 883 GCATGT [GA]CATG^[CT]
PpuMI 535 AGGACCC [GA]G^G[AT]CC[CT]
PvuII 1442 CAGCTG CAG^CTG
SacII 39 CCGCGG CCGC^GG
SapI 753 GAAGAGC GCTCTTC(1/4)
ScrFI 1166 CCAGG CC^[ACGT]GG
SmlI 406 CTTAAG C^T[CT][GA]AG
StyI 1348 CCATGG C^C[AT][AT]GG
XhoII 355 AGATCC [GA]^GATC[CT]
XmnI 1170 GAAAGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AfaI 324 GTAC GT^AC
433 GTAC
AocI 531 CCTCAGG CC^T[ACGT]AGG
1388 CCTCAGG
BbsI 300 GAAGAC GAAGAC(2/6)
1657 GAAGAC
BfaI 65 CTAG C^TAG
76 CTAG
BmyI 3 GGGCAC G[AGT]GC[ACT]^C
1648 GTGCAC
BpuAI 300 GAAGAC GAAGAC(2/6)
1657 GAAGAC
BsaI 892 GAGACC GGTCTC(1/5)
1501 GGTCTC
Bsp1286I 3 GGGCAC G[AGT]GC[ACT]^C
1648 GTGCAC
Bsu36I 531 CCTCAGG CC^T[ACGT]AGG
1388 CCTCAGG
DsaI 39 CCGCGG C^C[GA][CT]GG
1348 CCATGG
Eco81I 531 CCTCAGG CC^T[ACGT]AGG
1388 CCTCAGG
MaeI 65 CTAG C^TAG
76 CTAG
MaeII 1131 ACGT A^CGT
1275 ACGT
MslI 260 CATGTGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
385 CACAAAAATG
MspA1I 39 CCGCGG C[AC]G^C[GT]G
1442 CAGCTG
MwoI 1444 GCTGAGGTAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
1453 GCAAGAACCGC
PstI 230 CTGCAG CTGCA^G
471 CTGCAG
RsaI 324 GTAC GT^AC
433 GTAC
SfcI 230 CTGCAG C^T[GA][CT]AG
471 CTGCAG
#########################################
# 3 OR MORE CUT ENZYMES ( 42 enzymes) #
#########################################
AciI AcsI AluI AlwI ApoI
AvaII BbvI BciVI BpmI BsaJI
BsmAI BsmFI BspHI BsrI BstF5I
Cfr13I DdeI DpnII Fnu4HI FokI
HinfI HphI ItaI MaeIII MboI
MboII MnlI MseI NdeII NlaIII
NlaIV PleI RcaI Sau3AI Sau96I
SfaNI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI