Restriction map for
pFN21AE3325
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# NO CUT ENZYMES ( 72 enzymes) #
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Acc65I AccI AclI AcsI AflII
AgeI ApoI AscI AseI AsnI
Asp718I AvrII BciVI BfrI BlnI
BsaBI BsiWI BsmI BspDI BspHI
BsrDI BssHII Bst1107I BstAPI BstBI
BstZ17I ClaI DraI DrdI EcoRI
EcoRV EcoT22I FseI HindIII HpaI
KpnI KspI MamI MfeI MluI
MunI NcoI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmeI PshAI PshBI Psp1406I
PvuI RcaI SacII SalI SbfI
SexAI SfiI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspI SwaI
XbaI XhoI
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# ONE CUT ENZYMES ( 62 enzymes) #
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AflIII 377 ACATGT A^C[GA][CT]GT
AhdI 2269 GACCTCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 2130 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 363 CCTCAGG CC^T[ACGT]AGG
ApaI 563 GGGCCC GGGCC^C
AspEI 2269 GACCTCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1326 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 482 GGATCC G^GATCC
BbeI 1674 GGCGCC GGCGC^C
BbrPI 580 CACGTG CAC^GTG
BbsI 2287 GTCTTC GAAGAC(2/6)
BbvCI 2592 CCTCAGC CCTCAGC(-5/-2)
BcgI 1113 GCAGATCCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1289 TGATCA T^GATCA
BfaI 1526 CTAG C^TAG
BglI 2597 GCCAGATTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1128 AGATCT A^GATCT
BlpI 252 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 252 GCTGAGC GC^T[ACGT]AGC
BpuAI 2287 GTCTTC GAAGAC(2/6)
BsaAI 580 CACGTG [CT]AC^GT[GA]
BsaI 2664 GAGACC GGTCTC(1/5)
BsgI 1387 CTGCAC GTGCAG(16/14)
BsiEI 1959 CGGCCG CG[GA][CT]^CG
Bsp1407I 572 TGTACA T^GTACA
BspLU11I 377 ACATGT A^CATGT
BsrBI 1008 CCGCTC CCGCTC(-3/-3)
BsrGI 572 TGTACA T^GTACA
BssSI 853 CACGAG CACGAG(-5/-1)
BstEII 1698 GGTGACC G^GT[ACGT]ACC
BstPI 1698 GGTGACC G^GT[ACGT]ACC
BstXI 2678 CCAAGCTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 363 CCTCAGG CC^T[ACGT]AGG
CelII 252 GCTGAGC GC^T[ACGT]AGC
CpoI 683 CGGTCCG CG^G[AT]CCG
EagI 1959 CGGCCG C^GGCCG
Eam1105I 2269 GACCTCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1959 CGGCCG C^GGCCG
Eco52I 1959 CGGCCG C^GGCCG
Eco81I 363 CCTCAGG CC^T[ACGT]AGG
EcoO65I 1698 GGTGACC G^GT[ACGT]ACC
FbaI 1289 TGATCA T^GATCA
HincII 2191 GTCAAC GT[CT]^[GA]AC
HindII 2191 GTCAAC GT[CT]^[GA]AC
KasI 1674 GGCGCC G^GCGCC
MaeI 1526 CTAG C^TAG
MseI 1240 TTAA T^TAA
NaeI 116 GCCGGC GCC^GGC
NarI 1674 GGCGCC GG^CGCC
NgoMIV 116 GCCGGC G^CCGGC
PflFI 1326 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 580 CACGTG CAC^GTG
PmlI 580 CACGTG CAC^GTG
RsrII 683 CGGTCCG CG^G[AT]CCG
SacI 1921 GAGCTC GAGCT^C
SapI 909 GAAGAGC GCTCTTC(1/4)
SfoI 1674 GGCGCC GGC^GCC
SspBI 572 TGTACA T^GTACA
StuI 2027 AGGCCT AGG^CCT
Tru9I 1240 TTAA T^TAA
Tth111I 1326 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 2174 CCAAAATCGTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 25 enzymes) #
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AatII 1485 GACGTC GACGT^C
2479 GACGTC
AccIII 80 TCCGGA T^CCGGA
1322 TCCGGA
Alw44I 1185 GTGCAC G^TGCAC
1566 GTGCAC
Aor51HI 977 AGCGCT AGC^GCT
998 AGCGCT
ApaLI 1185 GTGCAC G^TGCAC
1566 GTGCAC
AviII 215 TGCGCA TGC^GCA
2378 TGCGCA
BalI 1979 TGGCCA TGG^CCA
2117 TGGCCA
BseAI 80 TCCGGA T^CCGGA
1322 TCCGGA
BsmBI 433 GAGACG CGTCTC(1/5)
1660 GAGACG
BspEI 80 TCCGGA T^CCGGA
1322 TCCGGA
BspMI 1385 ACCTGC ACCTGC(4/8)
1592 ACCTGC
BsrFI 116 GCCGGC [GA]^CCGG[CT]
1650 GCCGGT
Cfr10I 116 GCCGGC [GA]^CCGG[CT]
1650 GCCGGT
DraIII 474 CACCCCGTG CAC[ACGT][ACGT][ACGT]^GTG
871 CACCGCGTG
Eco47III 977 AGCGCT AGC^GCT
998 AGCGCT
EcoT14I 2249 CCAAGG C^C[AT][AT]GG
2490 CCTTGG
FspI 215 TGCGCA TGC^GCA
2378 TGCGCA
MluNI 1979 TGGCCA TGG^CCA
2117 TGGCCA
MroI 80 TCCGGA T^CCGGA
1322 TCCGGA
MscI 1979 TGGCCA TGG^CCA
2117 TGGCCA
NspI 377 ACATGT [GA]CATG^[CT]
2690 ACATGC
PstI 2350 CTGCAG CTGCA^G
2753 CTGCAG
ScaI 107 AGTACT AGT^ACT
1166 AGTACT
StyI 2249 CCAAGG C^C[AT][AT]GG
2490 CCTTGG
Tsp509I 1740 AATT ^AATT
2220 AATT
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# 3 OR MORE CUT ENZYMES ( 99 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI Asp700I AspHI AvaI AvaII
BanI BanII BbvI BcnI BmyI
BpmI BsaHI BsaJI BsaWI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoNI EcoO109I EcoRII
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeII MaeIII MboI MboII MflI
MnlI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PflMI PleI PpuMI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI SmlI TaqI TfiI
TseI Tsp45I TspRI TthHB8I Van91I
XhoII XmaCI XmaI XmnI