Restriction map for
pFN21AE3312
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# NO CUT ENZYMES ( 121 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I Aor51HI ApaI ApaLI AscI
Asp700I Asp718I AspEI AviII BamHI
BanI BbeI BbrPI BbvCI BcgI
BciVI BclI BfrI BglI BsaAI
BsaBI BsaHI BsaWI BseAI BsgI
BsiEI BsiWI BsmI BspDI BspEI
BspLU11I BsrDI BssHII BssSI BstBI
BstEII BstPI BstUI BstXI CfoI
ClaI CpoI DraIII EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I EcoRV
EcoT22I FbaI FseI FspI HaeII
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI KasI KpnI KspI
MamI MluI MroI MspA1I MvnI
NaeI NarI NdeI NgoMIV NotI
NruI NsiI NspV PacI PaeR7I
PflMI PinAI PmaCI PmeI PmlI
PshAI Psp1406I PvuI PvuII RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SmaI SnaBI Sse8387I SwaI Van91I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccI 2325 GTATAC GT^[AC][GT]AC
AocI 765 CCTGAGG CC^T[ACGT]AGG
AseI 1975 ATTAAT AT^TAAT
AsnI 1975 ATTAAT AT^TAAT
AspI 1291 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 458 CCCGAG C^[CT]CG[GA]G
AvrII 447 CCTAGG C^CTAGG
BalI 596 TGGCCA TGG^CCA
BcnI 2043 CCCGG CC^[GC]GG
BlnI 447 CCTAGG C^CTAGG
BlpI 723 GCTGAGC GC^T[ACGT]AGC
BpmI 887 CTGGAG CTGGAG(16/14)
Bpu1102I 723 GCTGAGC GC^T[ACGT]AGC
BsaI 1006 GGTCTC GGTCTC(1/5)
BsmBI 1775 CGTCTC CGTCTC(1/5)
BsoBI 458 CCCGAG C^[CT]CG[GA]G
Bsp1407I 336 TGTACA T^GTACA
BspHI 906 TCATGA T^CATGA
BsrBI 136 GAGCGG CCGCTC(-3/-3)
BsrFI 74 ACCGGC [GA]^CCGG[CT]
BsrGI 336 TGTACA T^GTACA
Bst1107I 2325 GTATAC GTA^TAC
BstAPI 300 GCAGCTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 2325 GTATAC GTA^TAC
Bsu36I 765 CCTGAGG CC^T[ACGT]AGG
CelII 723 GCTGAGC GC^T[ACGT]AGC
Cfr10I 74 ACCGGC [GA]^CCGG[CT]
DraI 2143 TTTAAA TTT^AAA
DrdI 1849 GACTGCAGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 347 CCATGG C^C[GA][CT]GG
Eco81I 765 CCTGAGG CC^T[ACGT]AGG
EcoNI 1111 CCTGCAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
MfeI 1990 CAATTG C^AATTG
MluNI 596 TGGCCA TGG^CCA
MscI 596 TGGCCA TGG^CCA
MunI 1990 CAATTG C^AATTG
NciI 2043 CCCGG CC^[GC]GG
NcoI 347 CCATGG C^CATGG
NheI 487 GCTAGC G^CTAGC
NspI 609 GCATGC [GA]CATG^[CT]
PflFI 1291 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 443 GGGACCT [GA]G^G[AT]CC[CT]
PshBI 1975 ATTAAT AT^TAAT
RcaI 906 TCATGA T^CATGA
SgrAI 73 CACCGGCG C[GA]^CCGG[CT]G
SpeI 2193 ACTAGT A^CTAGT
SphI 609 GCATGC GCATG^C
SspBI 336 TGTACA T^GTACA
SspI 1096 AATATT AAT^ATT
StuI 733 AGGCCT AGG^CCT
Tth111I 1291 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 17 enzymes) #
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AlwI 1368 GGATC GGATC(4/5)
1689 GGATC
AspHI 1495 GAGCAC G[AT]GC[AT]^C
2308 GAGCAC
BbsI 1813 GAAGAC GAAGAC(2/6)
409 GTCTTC
BglII 390 AGATCT A^GATCT
1527 AGATCT
BpuAI 1813 GAAGAC GAAGAC(2/6)
409 GTCTTC
BsiHKAI 1495 GAGCAC G[AT]GC[AT]^C
2308 GAGCAC
BstYI 390 AGATCT [GA]^GATC[CT]
1527 AGATCT
EaeI 8 TGGCCG [CT]^GGCC[GA]
596 TGGCCA
EcoRI 633 GAATTC G^AATTC
2065 GAATTC
HapII 75 CCGG C^CGG
2044 CCGG
HpaII 75 CCGG C^CGG
2044 CCGG
MaeII 1629 ACGT A^CGT
2298 ACGT
MflI 390 AGATCT [GA]^GATC[CT]
1527 AGATCT
MslI 240 CATTGTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2311 CACATGGGTG
MspI 75 CCGG C^CGG
2044 CCGG
NlaIV 443 GGGACC GG[ACGT]^[ACGT]CC
943 GGCTCC
XhoII 390 AGATCT [GA]^GATC[CT]
1527 AGATCT
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# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwNI
ApoI AvaII BanII BbvI BfaI
BmyI BsaJI BseRI BsiYI BslI
BsmAI BsmFI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI Cac8I Cfr13I
DdeI DpnII DraII EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HgaI HinfI HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MnlI MseI MvaI MwoI NdeII
NlaIII PleI PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I