Restriction map for
pFN21AE2044
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# NO CUT ENZYMES ( 90 enzymes) #
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AatII Acc65I AccII AclI AflII
AflIII AgeI Alw44I ApaLI AscI
AseI AsnI Asp700I Asp718I AspI
BbrPI BclI BfrI BglI BglII
BsaAI BsaBI BsiEI BsiWI BsmI
BspDI BspHI BspLU11I BsrBI BssHII
Bst1107I BstBI BstUI BstXI BstZ17I
ClaI CpoI DraI EagI EclXI
Eco52I EcoT22I FbaI FseI HincII
HindII HindIII HpaI KpnI KspI
MamI MfeI MluI MunI MvnI
NdeI NheI NotI NruI NsiI
NspV PacI PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SapI ScaI SfiI SfuI
SgrAI SnaBI SpeI SphI SspI
StuI SwaI Tth111I XbaI XmnI
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# ONE CUT ENZYMES ( 65 enzymes) #
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AccI 1290 GTCTAC GT^[AC][GT]AC
AccIII 108 TCCGGA T^CCGGA
AcsI 619 GAATTC [GA]^AATT[CT]
AfaI 324 GTAC GT^AC
AhdI 1378 GACTCTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 1331 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 1604 AGCGCT AGC^GCT
ApaI 135 GGGCCC GGGCC^C
ApoI 619 GAATTC [GA]^AATT[CT]
AspEI 1378 GACTCTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 1263 TGCGCA TGC^GCA
AvrII 139 CCTAGG C^CTAGG
BalI 1072 TGGCCA TGG^CCA
BamHI 1173 GGATCC G^GATCC
BbeI 822 GGCGCC GGCGC^C
BcgI 1673 GCATCCTTCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1938 GGATAC GTATCC(6/5)
BfaI 140 CTAG C^TAG
BlnI 139 CCTAGG C^CTAGG
BseAI 108 TCCGGA T^CCGGA
BsmBI 1353 CGTCTC CGTCTC(1/5)
Bsp1407I 323 TGTACA T^GTACA
BspEI 108 TCCGGA T^CCGGA
BsrFI 271 GCCGGC [GA]^CCGG[CT]
BsrGI 323 TGTACA T^GTACA
BssSI 1681 CTCGTG CACGAG(-5/-1)
BstAPI 1485 GCATCCCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 1173 GGATCC [GA]^GATC[CT]
Cfr10I 271 GCCGGC [GA]^CCGG[CT]
DrdI 366 GACAGAGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 538 CCATGG C^C[GA][CT]GG
Eam1105I 1378 GACTCTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1604 AGCGCT AGC^GCT
EcoRI 619 GAATTC G^AATTC
EcoRV 162 GATATC GAT^ATC
FspI 1263 TGCGCA TGC^GCA
KasI 822 GGCGCC G^GCGCC
MaeI 140 CTAG C^TAG
MflI 1173 GGATCC [GA]^GATC[CT]
MluNI 1072 TGGCCA TGG^CCA
MroI 108 TCCGGA T^CCGGA
MscI 1072 TGGCCA TGG^CCA
MseI 410 TTAA T^TAA
NaeI 271 GCCGGC GCC^GGC
NarI 822 GGCGCC GG^CGCC
NcoI 538 CCATGG C^CATGG
NgoMIV 271 GCCGGC G^CCGGC
NspI 860 ACATGC [GA]CATG^[CT]
PaeR7I 1894 CTCGAG C^TCGAG
PflMI 1064 CCATGTATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RsaI 324 GTAC GT^AC
SbfI 1836 CCTGCAGG CCTGCA^GG
SexAI 491 ACCAGGT A^CC[AT]GGT
SfoI 822 GGCGCC GGC^GCC
SmaI 1079 CCCGGG CCC^GGG
SmlI 1894 CTCGAG C^T[CT][GA]AG
Sse8387I 1836 CCTGCAGG CCTGCA^GG
SspBI 323 TGTACA T^GTACA
Tru9I 410 TTAA T^TAA
Van91I 1064 CCATGTATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 966 CCAATTTGGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1894 CTCGAG C^TCGAG
XhoII 1173 GGATCC [GA]^GATC[CT]
XmaCI 1079 CCCGGG C^CCGGG
XmaI 1079 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 18 enzymes) #
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AspHI 183 GTGCTC G[AT]GC[AT]^C
1413 GAGCAC
BanII 135 GGGCCC G[GA]GC[CT]^C
1082 GGGCTC
BbsI 1462 GAAGAC GAAGAC(2/6)
1899 GAAGAC
BlpI 275 GCTCAGC GC^T[ACGT]AGC
1410 GCTGAGC
Bpu1102I 275 GCTCAGC GC^T[ACGT]AGC
1410 GCTGAGC
BpuAI 1462 GAAGAC GAAGAC(2/6)
1899 GAAGAC
BsaI 478 GAGACC GGTCTC(1/5)
1504 GAGACC
BsaWI 108 TCCGGA [AT]^CCGG[AT]
521 ACCGGA
BsiHKAI 183 GTGCTC G[AT]GC[AT]^C
1413 GAGCAC
BsrDI 1219 GCAATG GCAATG(2/0)
1322 GCAATG
BstEII 12 GGTGACC G^GT[ACGT]ACC
384 GGTGACC
BstPI 12 GGTGACC G^GT[ACGT]ACC
384 GGTGACC
CelII 275 GCTCAGC GC^T[ACGT]AGC
1410 GCTGAGC
EaeI 452 TGGCCG [CT]^GGCC[GA]
1072 TGGCCA
EcoNI 350 CCTGGGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1836 CCTGCAGGAGG
EcoO65I 12 GGTGACC G^GT[ACGT]ACC
384 GGTGACC
MslI 435 CATCAAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1614 CATCGAAGTG
Tsp509I 620 AATT ^AATT
968 AATT
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# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AciI AcyI AluI AlwI AocI
AvaI AvaII BanI BbvCI BbvI
BcnI BmyI BpmI BsaHI BsaJI
BseRI BsgI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI Bsu36I Cac8I
CfoI Cfr13I DdeI DpnII DraII
DraIII EarI Eco81I EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeII MaeIII MboI MboII
MnlI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PleI
PpuMI PstI PvuII Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TaqI
TfiI TseI Tsp45I TspRI TthHB8I