Restriction map for
pFN21AE2265
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# NO CUT ENZYMES ( 134 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AflII AflIII AhdI Alw44I
AocI Aor51HI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AspI AvaI AviII AvrII BalI
BbeI BbrPI BcgI BclI BfrI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BseAI BseRI BsiEI BsiHKAI
BsiWI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrGI BssHII
BssSI Bst1107I BstUI BstXI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DrdI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoRI
EcoRV EcoT22I FbaI FseI FspI
HincII HindII HpaI KasI KpnI
KspI MamI MfeI MluI MluNI
MroI MscI MspA1I MunI MvnI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI PacI PaeR7I
PflFI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI ScaI SexAI SfiI SfoI
SgrAI SmaI SnaBI SpeI SphI
SspBI StuI SwaI Tth111I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AciI 531 GCGG CCGC(-3/-1)
AcsI 1253 AAATTC [GA]^AATT[CT]
AcyI 505 GGCGTC G[GA]^CG[CT]C
AfaI 548 GTAC GT^AC
AgeI 177 ACCGGT A^CCGGT
ApaI 290 GGGCCC GGGCC^C
ApoI 1253 AAATTC [GA]^AATT[CT]
AvaII 469 GGACC G^G[AT]CC
BamHI 270 GGATCC G^GATCC
BanI 857 GGTGCC G^G[CT][GA]CC
BanII 290 GGGCCC G[GA]GC[CT]^C
BcnI 267 CCCGG CC^[GC]GG
BglI 1167 GCCAGAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI 290 GGGCCC G[AGT]GC[ACT]^C
BsaHI 505 GGCGTC G[GA]^CG[CT]C
BsaI 484 GAGACC GGTCTC(1/5)
BsgI 1258 CTGCAC GTGCAG(16/14)
BsmBI 190 GAGACG CGTCTC(1/5)
BsmI 1318 GAATGC GAATGC(1/-1)
Bsp1286I 290 GGGCCC G[AGT]GC[ACT]^C
BsrFI 177 ACCGGT [GA]^CCGG[CT]
BstBI 282 TTCGAA TT^CGAA
BstEII 580 GGTGACC G^GT[ACGT]ACC
BstPI 580 GGTGACC G^GT[ACGT]ACC
CfoI 172 GCGC GCG^C
Cfr10I 177 ACCGGT [GA]^CCGG[CT]
DraII 289 AGGGCCC [GA]G^G[ACGT]CC[CT]
DraIII 139 CACCGTGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO109I 289 AGGGCCC [GA]G^G[ACGT]CC[CT]
EcoO65I 580 GGTGACC G^GT[ACGT]ACC
FauI 531 GCGGG CCCGC(4/6)
HaeII 171 GGCGCT [GA]GCGC^[CT]
HhaI 172 GCGC GCG^C
Hin1I 505 GGCGTC G[GA]^CG[CT]C
HinP1I 172 GCGC G^CGC
HindIII 673 AAGCTT A^AGCTT
MaeII 632 ACGT A^CGT
MseI 973 TTAA T^TAA
NciI 267 CCCGG CC^[GC]GG
NspI 600 GCATGT [GA]CATG^[CT]
NspV 282 TTCGAA TT^CGAA
PinAI 177 ACCGGT A^CCGGT
RsaI 548 GTAC GT^AC
SbfI 210 CCTGCAGG CCTGCA^GG
SfuI 282 TTCGAA TT^CGAA
Sse8387I 210 CCTGCAGG CCTGCA^GG
SspI 901 AATATT AAT^ATT
Tru9I 973 TTAA T^TAA
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
BbsI 433 GAAGAC GAAGAC(2/6)
1245 GAAGAC
BbvCI 1087 CCTCAGC CCTCAGC(-5/-2)
219 GCTGAGG
BciVI 614 GTATCC GTATCC(6/5)
1083 GTATCC
BfaI 757 CTAG C^TAG
1136 CTAG
BpuAI 433 GAAGAC GAAGAC(2/6)
1245 GAAGAC
BsmAI 190 GAGAC GTCTC(1/5)
484 GAGAC
BsmFI 468 GGGAC GGGAC(10/14)
245 GTCCC
BsrDI 746 CATTGC GCAATG(2/0)
957 CATTGC
BsrI 639 CCAGT ACTGG(1/-1)
1270 CCAGT
BstAPI 204 GCAGCACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1186 GCAGAATCTGC
Cac8I 950 GCTGGC GC[ACGT]^[ACGT]GC
961 GCCTGC
EarI 1214 CTCTTC CTCTTC(1/4)
285 GAAGAG
HaeIII 291 GGCC GG^CC
953 GGCC
HgaI 192 GACGC GACGC(5/10)
506 GCGTC
Ksp632I 1214 CTCTTC CTCTTC(1/4)
285 GAAGAG
MaeI 757 CTAG C^TAG
1136 CTAG
NcoI 585 CCATGG C^CATGG
1240 CCATGG
PstI 211 CTGCAG CTGCA^G
1193 CTGCAG
SmlI 325 CTTGAG C^T[CT][GA]AG
832 CTTGAG
XcmI 861 CCAAATACACTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1107 CCAATGGAAAAATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AluI AlwI AlwNI BbvI BpmI
BsaJI BsaWI BsiYI BslI BspMI
BssKI BstF5I BstNI BstYI Cfr13I
DdeI DpnII DsaI EcoRII EcoT14I
Fnu4HI FokI HapII HinfI HpaII
HphI ItaI MaeIII MboI MboII
MflI MnlI MslI MspI MvaI
MwoI NdeII NlaIII NlaIV PflMI
PleI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TaqI TfiI TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII