##################################
# NO CUT ENZYMES (  185 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AcyI        AfaI        AflII       AflIII      
AgeI        AhdI        Alw44I      AlwI        Aor51HI     
ApaLI       ApoI        AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspHI       AspI        
AvaI        AvaII       AviII       AvrII       BalI        
BamHI       BbeI        BbrPI       BbsI        BbvCI       
BcgI        BciVI       BclI        BfaI        BfrI        
BglII       BlnI        BpuAI       BsaAI       BsaBI       
BsaHI       BsaI        BsaWI       BseAI       BseRI       
BsiEI       BsiHKAI     BsiWI       BsmAI       BsmBI       
BsmFI       BsmI        BsoBI       Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BspMI       BsrBI       
BsrDI       BsrFI       BsrGI       BssHII      BssSI       
Bst1107I    BstBI       BstEII      BstF5I      BstPI       
BstXI       BstYI       BstZ17I     Cfr10I      ClaI        
CpoI        DraI        DraIII      DrdI        DsaI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
EcoNI       EcoO65I     EcoRI       EcoRV       EcoT14I     
EcoT22I     FbaI        FokI        FseI        FspI        
HaeII       HgaI        Hin1I       HincII      HindII      
HindIII     HinfI       HpaI        KasI        KpnI        
KspI        MaeI        MaeII       MamI        MfeI        
MflI        MluI        MluNI       MroI        MscI        
MseI        MslI        MunI        NaeI        NarI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PleI        
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PvuI        RcaI        RsaI        
RsrII       SacI        SacII       SalI        SbfI        
ScaI        SexAI       SfaNI       SfoI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StyI        
SwaI        TaqI        TfiI        Tru9I       Tsp509I     
Tth111I     TthHB8I     Van91I      XbaI        XcmI        
XhoI        XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   31 enzymes) #
###################################

AccII           5  CGCG                 CG^CG
AlwNI         358  CAGTTTCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI          304  CCTCAGG              CC^T[ACGT]AGG
ApaI           53  GGGCCC               GGGCC^C
BanI          111  GGCACC               G^G[CT][GA]CC
BpmI          364  CTGGAG               CTGGAG(16/14)
BsgI           71  CTGCAC               GTGCAG(16/14)
BstAPI        178  GCAACCACTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI           5  CGCG                 CG^CG
Bsu36I        304  CCTCAGG              CC^T[ACGT]AGG
CfoI          145  GCGC                 GCG^C
DpnII         351  GATC                 ^GATC
DraII          53  GGGCCCC              [GA]G^G[ACGT]CC[CT]
EaeI           44  CGGCCA               [CT]^GGCC[GA]
EarI          132  CTCTTC               CTCTTC(1/4)
Eco81I        304  CCTCAGG              CC^T[ACGT]AGG
EcoO109I       53  GGGCCCC              [GA]G^G[ACGT]CC[CT]
FauI           29  CCCGC                CCCGC(4/6)
HhaI          145  GCGC                 GCG^C
HinP1I        145  GCGC                 G^CGC
HphI          385  GGTGA                GGTGA(8/7)
Ksp632I       132  CTCTTC               CTCTTC(1/4)
MboI          351  GATC                 ^GATC
MboII         133  TCTTC                GAAGA(8/7)
MvnI            5  CGCG                 CG^CG
NdeII         351  GATC                 ^GATC
NlaIII         33  CATG                 CATG^
PvuII         188  CAGCTG               CAG^CTG
SapI          131  GCTCTTC              GCTCTTC(1/4)
Sau3AI        351  GATC                 ^GATC
StuI          280  AGGCCT               AGG^CCT


###################################
# TWO CUT ENZYMES (   15 enzymes) #
###################################

BanII          53  GGGCCC               G[GA]GC[CT]^C
              204  GGGCTC              
BcnI           51  CCGGG                CC^[GC]GG
              149  CCCGG               
BlpI           94  GCTCAGC              GC^T[ACGT]AGC
              268  GCTGAGC             
Bpu1102I       94  GCTCAGC              GC^T[ACGT]AGC
              268  GCTGAGC             
BsiYI         236  CCATTACTGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              375  CCAGAACCAGG         
BslI          236  CCATTACTGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              375  CCAGAACCAGG         
BsrI          241  ACTGG                ACTGG(1/-1)
               88  CCAGT               
CelII          94  GCTCAGC              GC^T[ACGT]AGC
              268  GCTGAGC             
MaeIII        230  GTCAC                ^GT[ACGT]AC
              386  GTGAC               
MspA1I        107  CAGCGG               C[AC]G^C[GT]G
              188  CAGCTG              
NciI           51  CCGGG                CC^[GC]GG
              149  CCCGG               
PstI          122  CTGCAG               CTGCA^G
              185  CTGCAG              
SfcI          122  CTGCAG               C^T[GA][CT]AG
              185  CTGCAG              
SfiI           36  GGCCCCTCCGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
               45  GGCCAGCCGGGCC       
Tsp45I        230  GTCAC                ^GT[GC]AC
              386  GTGAC               


#########################################
# 3 OR MORE CUT ENZYMES (   27 enzymes) #
#########################################

AciI        AluI        BbvI        BglI        BmyI        
BsaJI       Bsp1286I    BssKI       BstNI       Cac8I       
Cfr13I      DdeI        EcoRII      Fnu4HI      HaeIII      
HapII       HpaII       ItaI        MnlI        MspI        
MvaI        MwoI        NlaIV       Sau96I      ScrFI       
TseI        TspRI