Restriction map for
pFN21AE4041
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# NO CUT ENZYMES ( 152 enzymes) #
##################################
AatII AccI AccII AccIII AciI
AclI AflII AgeI AhdI Alw44I
AocI ApaI ApaLI AscI AseI
AsnI Asp700I AspEI AspI AvaI
AviII AvrII BamHI BbeI BbvCI
BcgI BciVI BclI BcnI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaBI BsaI BsaWI BseAI BsiEI
BsiWI BsmI BsoBI BspDI BspEI
BspHI BspLU11I BsrBI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I CelII ClaI
CpoI DraI DraII DraIII DsaI
EagI Eam1105I EarI EclXI Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRV
EcoT14I EcoT22I FauI FbaI FseI
FspI HindIII HinfI HpaI KasI
Ksp632I KspI MamI MfeI MluI
MroI MseI MslI MspA1I MunI
MvnI NarI NciI NcoI NdeI
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmeI PpuMI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsrII SacII SalI SapI
SbfI ScaI SexAI SfcI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspI StuI
StyI SwaI TfiI Tru9I Tth111I
Van91I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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Acc65I 171 GGTACC G^GTACC
AcyI 481 GGCGTC G[GA]^CG[CT]C
AlwNI 302 CAGCCACTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 346 AGCGCT AGC^GCT
Asp718I 171 GGTACC G^GTACC
AvaII 473 GGTCC G^G[AT]CC
BalI 2 TGGCCA TGG^CCA
BanI 171 GGTACC G^G[CT][GA]CC
BbsI 325 GTCTTC GAAGAC(2/6)
BfaI 118 CTAG C^TAG
BpmI 583 CTGGAG CTGGAG(16/14)
BpuAI 325 GTCTTC GAAGAC(2/6)
BsaHI 481 GGCGTC G[GA]^CG[CT]C
BsgI 250 CTGCAC GTGCAG(16/14)
BsiYI 526 CCTGAGCATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 526 CCTGAGCATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 158 GAGAC GTCTC(1/5)
BsmBI 158 GAGACG CGTCTC(1/5)
BsmFI 474 GTCCC GGGAC(10/14)
Bsp1407I 76 TGTACA T^GTACA
BspMI 174 ACCTGC ACCTGC(4/8)
BsrDI 512 GCAATG GCAATG(2/0)
BsrFI 495 GCCGGC [GA]^CCGG[CT]
BsrGI 76 TGTACA T^GTACA
CfoI 347 GCGC GCG^C
Cfr10I 495 GCCGGC [GA]^CCGG[CT]
Cfr13I 473 GGTCC G^G[ACGT]CC
DrdI 670 GACAATAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 346 AGCGCT AGC^GCT
EcoRI 590 GAATTC G^AATTC
HaeII 346 AGCGCT [GA]GCGC^[CT]
HhaI 347 GCGC GCG^C
Hin1I 481 GGCGTC G[GA]^CG[CT]C
HinP1I 347 GCGC G^CGC
HincII 667 GTTGAC GT[CT]^[GA]AC
HindII 667 GTTGAC GT[CT]^[GA]AC
KpnI 171 GGTACC GGTAC^C
MaeI 118 CTAG C^TAG
MaeIII 289 GTGAC ^GT[ACGT]AC
MluNI 2 TGGCCA TGG^CCA
MscI 2 TGGCCA TGG^CCA
NaeI 495 GCCGGC GCC^GGC
NgoMIV 495 GCCGGC G^CCGGC
Sau96I 473 GGTCC G^G[ACGT]CC
SspBI 76 TGTACA T^GTACA
TaqI 156 TCGA T^CGA
Tsp45I 289 GTGAC ^GT[GC]AC
TthHB8I 156 TCGA T^CGA
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# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AcsI 590 GAATTC [GA]^AATT[CT]
623 GAATTT
AflIII 81 ACACGT A^C[GA][CT]GT
83 ACGTGT
ApoI 590 GAATTC [GA]^AATT[CT]
623 GAATTT
AspHI 265 GAGCTC G[AT]GC[AT]^C
742 GAGCTC
BanII 265 GAGCTC G[GA]GC[CT]^C
742 GAGCTC
BbrPI 82 CACGTG CAC^GTG
253 CACGTG
BbvI 222 GCAGC GCAGC(8/12)
301 GCAGC
BsaAI 82 CACGTG [CT]AC^GT[GA]
253 CACGTG
BseRI 423 CTCCTC GAGGAG(10/8)
262 GAGGAG
BsiHKAI 265 GAGCTC G[AT]GC[AT]^C
742 GAGCTC
BstYI 362 AGATCC [GA]^GATC[CT]
384 AGATCC
EaeI 2 TGGCCA [CT]^GGCC[GA]
429 CGGCCA
Fnu4HI 222 GCAGC GC^[ACGT]GC
301 GCAGC
HgaI 200 GCGTC GACGC(5/10)
482 GCGTC
HphI 165 GGTGA GGTGA(8/7)
462 GGTGA
ItaI 222 GCAGC GC^[ACGT]GC
301 GCAGC
MflI 362 AGATCC [GA]^GATC[CT]
384 AGATCC
PmaCI 82 CACGTG CAC^GTG
253 CACGTG
PmlI 82 CACGTG CAC^GTG
253 CACGTG
SacI 265 GAGCTC GAGCT^C
742 GAGCTC
SmlI 238 CTTGAG C^T[CT][GA]AG
739 CTTGAG
TseI 222 GCAGC G^C[AT]GC
301 GCAGC
Tsp509I 591 AATT ^AATT
624 AATT
TspRI 306 CACTG CA[GC]TG[ACGT][ACGT]^
352 CAGTG
XhoII 362 AGATCC [GA]^GATC[CT]
384 AGATCC
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# 3 OR MORE CUT ENZYMES ( 33 enzymes) #
#########################################
AfaI AluI AlwI BmyI BsaJI
Bsp1286I BsrI BssKI BstAPI BstF5I
BstNI Cac8I DdeI DpnII EcoRII
FokI HaeIII HapII HpaII MaeII
MboI MboII MnlI MspI MvaI
MwoI NdeII NlaIII NlaIV RsaI
Sau3AI ScrFI SfaNI