Restriction map for
pFN21AE6162
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# NO CUT ENZYMES ( 186 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AfaI AflII
AgeI AhdI Alw44I AlwI AlwNI
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BamHI
BanI BbeI BbrPI BbsI BbvCI
BcgI BciVI BclI BcnI BfaI
BfrI BglI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BsgI BsiEI
BsiWI BsmBI Bsp1407I BspDI BspEI
BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstYI BstZ17I Bsu36I CelII CfoI
Cfr10I ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FbaI FseI
FspI HaeII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HpaI HpaII HphI KasI KpnI
KspI MaeI MamI MflI MluI
MroI MseI MspA1I MspI MvnI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsaI RsrII SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI TaqI TfiI
Tru9I Tth111I TthHB8I Van91I XbaI
XcmI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 34 enzymes) #
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AflIII 176 ACATGT A^C[GA][CT]GT
AvaI 397 CTCGGG C^[CT]CG[GA]G
BalI 2 TGGCCA TGG^CCA
BsmAI 491 GAGAC GTCTC(1/5)
BsmI 121 GCATTC GAATGC(1/-1)
BsoBI 397 CTCGGG C^[CT]CG[GA]G
BspLU11I 176 ACATGT A^CATGT
BsrI 349 ACTGG ACTGG(1/-1)
BstF5I 267 CATCC GGATG(2/0)
DpnII 117 GATC ^GATC
DsaI 74 CCGTGG C^C[GA][CT]GG
EaeI 2 TGGCCA [CT]^GGCC[GA]
EarI 157 CTCTTC CTCTTC(1/4)
FauI 195 CCCGC CCCGC(4/6)
FokI 267 CATCC GGATG(9/13)
HinfI 240 GAGTC G^A[ACGT]TC
Ksp632I 157 CTCTTC CTCTTC(1/4)
MaeII 388 ACGT A^CGT
MaeIII 242 GTCAC ^GT[ACGT]AC
MboI 117 GATC ^GATC
MfeI 505 CAATTG C^AATTG
MluNI 2 TGGCCA TGG^CCA
MscI 2 TGGCCA TGG^CCA
MslI 69 CATTCCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 505 CAATTG C^AATTG
NdeII 117 GATC ^GATC
PleI 240 GAGTC GAGTC(4/5)
SapI 156 GCTCTTC GCTCTTC(1/4)
Sau3AI 117 GATC ^GATC
SfaNI 266 GCATC GCATC(5/9)
SfcI 33 CTACAG C^T[GA][CT]AG
Tsp45I 242 GTCAC ^GT[GC]AC
Tsp509I 506 AATT ^AATT
TspRI 329 CAGTG CA[GC]TG[ACGT][ACGT]^
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# TWO CUT ENZYMES ( 18 enzymes) #
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AspHI 324 GAGCTC G[AT]GC[AT]^C
378 GAGCTC
BanII 324 GAGCTC G[GA]GC[CT]^C
378 GAGCTC
BbvI 139 GCTGC GCAGC(8/12)
153 GCTGC
BmyI 324 GAGCTC G[AGT]GC[ACT]^C
378 GAGCTC
BpmI 237 CTGGAG CTGGAG(16/14)
488 CTGGAG
BseRI 381 CTCCTC GAGGAG(10/8)
424 CTCCTC
BsiHKAI 324 GAGCTC G[AT]GC[AT]^C
378 GAGCTC
BsmFI 10 GGGAC GGGAC(10/14)
452 GTCCC
Bsp1286I 324 GAGCTC G[AGT]GC[ACT]^C
378 GAGCTC
DdeI 317 CTCAG C^T[ACGT]AG
327 CTCAG
DraII 450 GGGTCCC [GA]G^G[ACGT]CC[CT]
496 AGGACCT
EcoO109I 450 GGGTCCC [GA]G^G[ACGT]CC[CT]
496 AGGACCT
MboII 84 GAAGA GAAGA(8/7)
158 TCTTC
NlaIII 177 CATG CATG^
230 CATG
NspI 176 ACATGT [GA]CATG^[CT]
229 ACATGC
PpuMI 450 GGGTCCC [GA]G^G[AT]CC[CT]
496 AGGACCT
SacI 324 GAGCTC GAGCT^C
378 GAGCTC
TseI 139 GCTGC G^C[AT]GC
153 GCTGC
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# 3 OR MORE CUT ENZYMES ( 20 enzymes) #
#########################################
AciI AluI AvaII BsaJI BsiYI
BslI BssKI BstNI Cac8I Cfr13I
EcoRII Fnu4HI HaeIII ItaI MnlI
MvaI MwoI NlaIV Sau96I ScrFI