Restriction map for
pFN21AE5739
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# NO CUT ENZYMES ( 150 enzymes) #
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Acc65I AccI AccII AccIII AflII
AflIII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspI
AvaI AviII BalI BamHI BanI
BbeI BbrPI BbsI BbvCI BcgI
BclI BcnI BfrI BglI BglII
BlpI Bpu1102I BpuAI BsaAI BsaWI
BseAI BsgI BsiEI BsiWI BsmFI
BsmI BsoBI BspEI BspHI BspLU11I
BsrBI BsrFI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstZ17I Bsu36I CelII CfoI Cfr10I
CpoI DraII DraIII DrdI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HaeII HaeIII HgaI HhaI HinP1I
HincII HindII HpaI KasI KpnI
KspI MfeI MluI MluNI MroI
MscI MunI MvnI MwoI NaeI
NarI NciI NgoMIV NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI PshBI PvuI
RcaI RsrII SacI SacII SalI
SbfI ScaI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
Sse8387I StuI SwaI Tth111I Van91I
XbaI XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AatII 39 GACGTC GACGT^C
AclI 482 AACGTT AA^CGTT
AcyI 39 GACGTC G[GA]^CG[CT]C
Asp700I 1583 GAAAGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 200 GTGCTC G[AT]GC[AT]^C
AvaII 186 GGACC G^G[AT]CC
AvrII 493 CCTAGG C^CTAGG
BanII 1563 GAGCCC G[GA]GC[CT]^C
BciVI 388 GGATAC GTATCC(6/5)
BlnI 493 CCTAGG C^CTAGG
BpmI 776 CTCCAG CTGGAG(16/14)
BsaBI 466 GATACATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 39 GACGTC G[GA]^CG[CT]C
BsaI 1456 GAGACC GGTCTC(1/5)
BsiHKAI 200 GTGCTC G[AT]GC[AT]^C
BsmBI 37 GAGACG CGTCTC(1/5)
Bsp1407I 1231 TGTACA T^GTACA
BspDI 845 ATCGAT AT^CGAT
BspMI 61 GCAGGT ACCTGC(4/8)
BsrDI 923 GCAATG GCAATG(2/0)
BsrGI 1231 TGTACA T^GTACA
BssKI 356 CCAGG ^CC[ACGT]GG
BstNI 356 CCAGG CC^[AT]GG
BstYI 742 GGATCT [GA]^GATC[CT]
Cfr13I 186 GGACC G^G[ACGT]CC
ClaI 845 ATCGAT AT^CGAT
DraI 1123 TTTAAA TTT^AAA
DsaI 1430 CCATGG C^C[GA][CT]GG
EcoRII 356 CCAGG ^CC[AT]GG
FauI 692 GCGGG CCCGC(4/6)
Hin1I 39 GACGTC G[GA]^CG[CT]C
HindIII 1585 AAGCTT A^AGCTT
MamI 466 GATACATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 742 GGATCT [GA]^GATC[CT]
MspA1I 1757 CAGCTG C[AC]G^C[GT]G
MvaI 356 CCAGG CC^[AT]GG
NcoI 1430 CCATGG C^CATGG
NheI 88 GCTAGC G^CTAGC
NspI 1378 GCATGC [GA]CATG^[CT]
Psp1406I 482 AACGTT AA^CGTT
PstI 59 CTGCAG CTGCA^G
PvuII 1757 CAGCTG CAG^CTG
Sau96I 186 GGACC G^G[ACGT]CC
ScrFI 356 CCAGG CC^[ACGT]GG
SexAI 355 ACCAGGT A^CC[AT]GGT
SphI 1378 GCATGC GCATG^C
SspBI 1231 TGTACA T^GTACA
SspI 1522 AATATT AAT^ATT
XhoII 742 GGATCT [GA]^GATC[CT]
XmnI 1583 GAAAGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 17 enzymes) #
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BsaJI 493 CCTAGG C^C[ACGT][ACGT]GG
1430 CCATGG
BstXI 189 CCATAATTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1736 CCAGTAGTTTGG
Cac8I 88 GCTAGC GC[ACGT]^[ACGT]GC
1378 GCATGC
EarI 979 CTCTTC CTCTTC(1/4)
1560 GAAGAG
EcoT14I 493 CCTAGG C^C[AT][AT]GG
1430 CCATGG
HapII 223 CCGG C^CGG
534 CCGG
HpaII 223 CCGG C^CGG
534 CCGG
Ksp632I 979 CTCTTC CTCTTC(1/4)
1560 GAAGAG
MspI 223 CCGG C^CGG
534 CCGG
NdeI 363 CATATG CA^TATG
1768 CATATG
SapI 1560 GAAGAGC GCTCTTC(1/4)
978 GCTCTTC
SfaNI 1054 GATGC GCATC(5/9)
1186 GATGC
SfcI 59 CTGCAG C^T[GA][CT]AG
992 CTGTAG
StyI 493 CCTAGG C^C[AT][AT]GG
1430 CCATGG
TaqI 846 TCGA T^CGA
1212 TCGA
Tsp45I 404 GTGAC ^GT[GC]AC
1753 GTGAC
TthHB8I 846 TCGA T^CGA
1212 TCGA
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# 3 OR MORE CUT ENZYMES ( 41 enzymes) #
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AciI AcsI AfaI AluI AlwI
ApoI BbvI BfaI BmyI BseRI
BsiYI BslI BsmAI Bsp1286I BsrI
BstF5I DdeI DpnII Fnu4HI FokI
HinfI HphI ItaI MaeI MaeII
MaeIII MboI MboII MnlI MseI
MslI NdeII NlaIII NlaIV RsaI
Sau3AI TfiI Tru9I TseI Tsp509I
TspRI