Restriction map for
pFN21AE5142
##################################
# NO CUT ENZYMES ( 177 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AvaI
AviII AvrII BalI BamHI BanI
BbeI BbrPI BbsI BcgI BciVI
BclI BcnI BfaI BfrI BglI
BglII BlnI BlpI Bpu1102I BpuAI
BsaAI BsaHI BsaWI BseAI BsiEI
BsiHKAI BsiWI BsmBI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrGI BsrI
BssHII BssSI Bst1107I BstAPI BstBI
BstUI BstXI BstYI BstZ17I CelII
CfoI ClaI CpoI DraI DraIII
DrdI EaeI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoRI EcoRV
EcoT22I FauI FbaI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HincII HindII HindIII HpaI KasI
KpnI KspI MaeI MaeII MfeI
MflI MluI MluNI MroI MscI
MspA1I MunI MvnI MwoI NaeI
NarI NciI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TaqI TfiI Tth111I
TthHB8I XbaI XhoI XhoII XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
AciI 162 CCGC CCGC(-3/-1)
AcsI 73 AAATTT [GA]^AATT[CT]
AfaI 159 GTAC GT^AC
AluI 35 AGCT AG^CT
AlwI 145 GATCC GGATC(4/5)
AocI 126 CCTGAGG CC^T[ACGT]AGG
ApoI 73 AAATTT [GA]^AATT[CT]
BanII 190 GAGCCC G[GA]GC[CT]^C
BbvCI 174 CCTCAGC CCTCAGC(-5/-2)
BpmI 31 CTCCAG CTGGAG(16/14)
BsaBI 228 GATCAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 91 GAGACC GGTCTC(1/5)
BsaJI 194 CCATGG C^C[ACGT][ACGT]GG
BseRI 279 CTCCTC GAGGAG(10/8)
BsgI 302 CTGCAC GTGCAG(16/14)
BsiYI 33 CCAGCTCTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 33 CCAGCTCTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 91 GAGAC GTCTC(1/5)
BsmFI 238 GGGAC GGGAC(10/14)
BsrFI 274 ACCGGC [GA]^CCGG[CT]
BssKI 258 CCTGG ^CC[ACGT]GG
BstEII 169 GGTGACC G^GT[ACGT]ACC
BstNI 258 CCTGG CC^[AT]GG
BstPI 169 GGTGACC G^GT[ACGT]ACC
Bsu36I 126 CCTGAGG CC^T[ACGT]AGG
Cac8I 110 GCTTGC GC[ACGT]^[ACGT]GC
Cfr10I 274 ACCGGC [GA]^CCGG[CT]
DraII 130 AGGACCC [GA]G^G[ACGT]CC[CT]
DsaI 194 CCATGG C^C[GA][CT]GG
EarI 287 CTCTTC CTCTTC(1/4)
Eco81I 126 CCTGAGG CC^T[ACGT]AGG
EcoO109I 130 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoO65I 169 GGTGACC G^GT[ACGT]ACC
EcoRII 258 CCTGG ^CC[AT]GG
EcoT14I 194 CCATGG C^C[AT][AT]GG
HaeIII 198 GGCC GG^CC
HapII 275 CCGG C^CGG
HinfI 240 GACTC G^A[ACGT]TC
HpaII 275 CCGG C^CGG
Ksp632I 287 CTCTTC CTCTTC(1/4)
MamI 228 GATCAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 77 TTAA T^TAA
MslI 136 CACAAGTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 275 CCGG C^CGG
MvaI 258 CCTGG CC^[AT]GG
NcoI 194 CCATGG C^CATGG
PflMI 33 CCAGCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 240 GACTC GAGTC(4/5)
PpuMI 130 AGGACCC [GA]G^G[AT]CC[CT]
RsaI 159 GTAC GT^AC
ScrFI 258 CCTGG CC^[ACGT]GG
StyI 194 CCATGG C^C[AT][AT]GG
Tru9I 77 TTAA T^TAA
Tsp509I 74 AATT ^AATT
TspRI 50 CAGTG CA[GC]TG[ACGT][ACGT]^
Van91I 33 CCAGCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 194 CCATGGCCTACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AvaII 131 GGACC G^G[AT]CC
261 GGTCC
BbvI 46 GCAGC GCAGC(8/12)
211 GCTGC
BmyI 61 GTGCCC G[AGT]GC[ACT]^C
190 GAGCCC
Bsp1286I 61 GTGCCC G[AGT]GC[ACT]^C
190 GAGCCC
BstF5I 8 GGATG GGATG(2/0)
207 GGATG
Cfr13I 131 GGACC G^G[ACGT]CC
261 GGTCC
DdeI 127 CTGAG C^T[ACGT]AG
175 CTCAG
DpnII 145 GATC ^GATC
228 GATC
Fnu4HI 46 GCAGC GC^[ACGT]GC
211 GCTGC
FokI 8 GGATG GGATG(9/13)
207 GGATG
HphI 169 GGTGA GGTGA(8/7)
223 GGTGA
ItaI 46 GCAGC GC^[ACGT]GC
211 GCTGC
MaeIII 170 GTGAC ^GT[ACGT]AC
181 GTGAC
MboI 145 GATC ^GATC
228 GATC
MboII 69 GAAGA GAAGA(8/7)
288 TCTTC
NdeII 145 GATC ^GATC
228 GATC
NlaIV 131 GGACCC GG[ACGT]^[ACGT]CC
277 GGCTCC
Sau3AI 145 GATC ^GATC
228 GATC
Sau96I 131 GGACC G^G[ACGT]CC
261 GGTCC
SfaNI 208 GATGC GCATC(5/9)
233 GCATC
TseI 46 GCAGC G^C[AT]GC
211 GCTGC
Tsp45I 170 GTGAC ^GT[GC]AC
181 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 2 enzymes) #
#########################################
MnlI NlaIII