Restriction map for
pFN21AE4414
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# NO CUT ENZYMES ( 120 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AfaI AflII AgeI AhdI AocI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvrII BamHI
BbsI BbvCI BcgI BciVI BclI
BfaI BfrI BglII BlnI BpuAI
BsaI BseAI BsiEI BsiWI BsmI
Bsp1407I BspDI BspEI BspLU11I BsrBI
BsrDI BsrGI BssHII BstBI BstEII
BstPI BstXI Bsu36I ClaI CpoI
DraI DrdI EagI Eam1105I EarI
EclXI Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoT22I FbaI FseI HincII
HindII HpaI KpnI Ksp632I KspI
MaeI MboII MfeI MroI MslI
MunI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmeI PpuMI PshAI PshBI
Psp1406I PvuI RsaI RsrII SacI
SacII SalI SapI SbfI ScaI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SspBI SspI StuI SwaI
TaqI Tsp509I Tth111I TthHB8I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccI 918 GTATAC GT^[AC][GT]AC
AflIII 1046 ACGCGT A^C[GA][CT]GT
AvaI 947 CCCGAG C^[CT]CG[GA]G
AvaII 249 GGTCC G^G[AT]CC
AviII 506 TGCGCA TGC^GCA
BbrPI 889 CACGTG CAC^GTG
BlpI 1227 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 1227 GCTTAGC GC^T[ACGT]AGC
BsaAI 889 CACGTG [CT]AC^GT[GA]
BsaWI 1073 ACCGGA [AT]^CCGG[AT]
BseRI 214 CTCCTC GAGGAG(10/8)
BsoBI 947 CCCGAG C^[CT]CG[GA]G
BspMI 1139 ACCTGC ACCTGC(4/8)
BsrFI 76 GCCGGC [GA]^CCGG[CT]
BssSI 156 CTCGTG CACGAG(-5/-1)
Bst1107I 918 GTATAC GTA^TAC
BstAPI 318 GCAGATCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 9 GGATCT [GA]^GATC[CT]
BstZ17I 918 GTATAC GTA^TAC
CelII 1227 GCTTAGC GC^T[ACGT]AGC
Cfr10I 76 GCCGGC [GA]^CCGG[CT]
DraIII 777 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoRV 910 GATATC GAT^ATC
FspI 506 TGCGCA TGC^GCA
HindIII 589 AAGCTT A^AGCTT
HphI 760 GGTGA GGTGA(8/7)
MaeIII 761 GTGAC ^GT[ACGT]AC
MflI 9 GGATCT [GA]^GATC[CT]
MluI 1046 ACGCGT A^CGCGT
NaeI 76 GCCGGC GCC^GGC
NcoI 1100 CCATGG C^CATGG
NgoMIV 76 GCCGGC G^CCGGC
NspI 126 GCATGC [GA]CATG^[CT]
PflMI 653 CCACCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 889 CACGTG CAC^GTG
PmlI 889 CACGTG CAC^GTG
PstI 1141 CTGCAG CTGCA^G
PvuII 253 CAGCTG CAG^CTG
SexAI 482 ACCTGGT A^CC[AT]GGT
SfcI 1141 CTGCAG C^T[GA][CT]AG
SfiI 846 GGCCGTGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 33 CTTGAG C^T[CT][GA]AG
SphI 126 GCATGC GCATG^C
Tsp45I 761 GTGAC ^GT[GC]AC
Van91I 653 CCACCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 9 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 27 enzymes) #
###################################
Alw44I 804 GTGCAC G^TGCAC
892 GTGCAC
AlwNI 98 CAGGAACTG CAG[ACGT][ACGT][ACGT]^CTG
1147 CAGAATCTG
Aor51HI 47 AGCGCT AGC^GCT
447 AGCGCT
ApaI 369 GGGCCC GGGCC^C
1169 GGGCCC
ApaLI 804 GTGCAC G^TGCAC
892 GTGCAC
BalI 461 TGGCCA TGG^CCA
584 TGGCCA
BanII 369 GGGCCC G[GA]GC[CT]^C
1169 GGGCCC
BbeI 357 GGCGCC GGCGC^C
396 GGCGCC
BglI 76 GCCGGCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
847 GCCGTGGTGGC
BsaBI 729 GATCCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
906 GATGGATATC
BsmBI 475 CGTCTC CGTCTC(1/5)
36 GAGACG
BsmFI 281 GGGAC GGGAC(10/14)
456 GTCCC
BspHI 260 TCATGA T^CATGA
902 TCATGA
BstF5I 968 GGATG GGATG(2/0)
1209 GGATG
Eco47III 47 AGCGCT AGC^GCT
447 AGCGCT
EcoT14I 438 CCAAGG C^C[AT][AT]GG
1100 CCATGG
FokI 968 GGATG GGATG(9/13)
1209 GGATG
KasI 357 GGCGCC G^GCGCC
396 GGCGCC
MaeII 764 ACGT A^CGT
890 ACGT
MamI 729 GATCCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
906 GATGGATATC
MluNI 461 TGGCCA TGG^CCA
584 TGGCCA
MscI 461 TGGCCA TGG^CCA
584 TGGCCA
NarI 357 GGCGCC GG^CGCC
396 GGCGCC
RcaI 260 TCATGA T^CATGA
902 TCATGA
SfoI 357 GGCGCC GGC^GCC
396 GGCGCC
StyI 438 CCAAGG C^C[AT][AT]GG
1100 CCATGG
TfiI 428 GATTC G^A[AT]TC
1149 GAATC
#########################################
# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AccII AciI AcyI AluI AlwI
AspHI BanI BbvI BcnI BmyI
BpmI BsaHI BsaJI BsgI BsiHKAI
BsiYI BslI BsmAI Bsp1286I BsrI
BssKI BstNI BstUI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EcoO109I EcoRII FauI Fnu4HI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII ItaI
MboI MnlI MseI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PleI Sau3AI Sau96I
ScrFI SfaNI Tru9I TseI TspRI