Restriction map for
pFN21AE3556
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# NO CUT ENZYMES ( 171 enzymes) #
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AatII Acc65I AccIII AclI AcyI
AfaI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AvaI
AviII AvrII BalI BamHI BanII
BbeI BbrPI BbvCI BcgI BciVI
BclI BfrI BglI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BseRI BsgI
BsiEI BsiHKAI BsiWI BsmBI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BstAPI BstBI BstEII
BstPI BstXI BstYI Bsu36I CelII
Cfr10I ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoT22I FauI FbaI FseI FspI
HaeII HgaI Hin1I HincII HindII
HpaI KasI KpnI Ksp632I KspI
MaeII MamI MfeI MflI MluI
MluNI MroI MscI MslI MunI
NaeI NarI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI RcaI RsaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tth111I Van91I XbaI
XcmI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AccI 633 GTATAC GT^[AC][GT]AC
AcsI 374 GAATTC [GA]^AATT[CT]
AlwI 580 GATCC GGATC(4/5)
ApoI 374 GAATTC [GA]^AATT[CT]
AvaII 234 GGACC G^G[AT]CC
BanI 567 GGTGCC G^G[CT][GA]CC
BbsI 174 GAAGAC GAAGAC(2/6)
BcnI 540 CCCGG CC^[GC]GG
BfaI 94 CTAG C^TAG
BmyI 568 GTGCCC G[AGT]GC[ACT]^C
BpmI 154 CTGGAG CTGGAG(16/14)
BpuAI 174 GAAGAC GAAGAC(2/6)
BsmAI 157 GAGAC GTCTC(1/5)
Bsp1286I 568 GTGCCC G[AGT]GC[ACT]^C
BssSI 29 CTCGTG CACGAG(-5/-1)
Bst1107I 633 GTATAC GTA^TAC
BstNI 238 CCTGG CC^[AT]GG
BstZ17I 633 GTATAC GTA^TAC
DdeI 430 CTCAG C^T[ACGT]AG
DsaI 612 CCATGG C^C[GA][CT]GG
EcoRI 374 GAATTC G^AATTC
EcoRII 238 CCTGG ^CC[AT]GG
EcoRV 256 GATATC GAT^ATC
EcoT14I 612 CCATGG C^C[AT][AT]GG
HapII 541 CCGG C^CGG
HindIII 460 AAGCTT A^AGCTT
HpaII 541 CCGG C^CGG
MaeI 94 CTAG C^TAG
MseI 476 TTAA T^TAA
MspA1I 443 CAGCTG C[AC]G^C[GT]G
MspI 541 CCGG C^CGG
MvaI 238 CCTGG CC^[AT]GG
NciI 540 CCCGG CC^[GC]GG
NcoI 612 CCATGG C^CATGG
NdeI 179 CATATG CA^TATG
PpuMI 233 GGGACCC [GA]G^G[AT]CC[CT]
PvuII 443 CAGCTG CAG^CTG
SfcI 316 CTACAG C^T[GA][CT]AG
StyI 612 CCATGG C^C[AT][AT]GG
TaqI 438 TCGA T^CGA
Tru9I 476 TTAA T^TAA
Tsp45I 197 GTGAC ^GT[GC]AC
TthHB8I 438 TCGA T^CGA
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# TWO CUT ENZYMES ( 20 enzymes) #
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AccII 6 CGCG CG^CG
248 CGCG
BbvI 442 GCAGC GCAGC(8/12)
676 GCTGC
BsmFI 233 GGGAC GGGAC(10/14)
293 GTCCC
BssKI 238 CCTGG ^CC[ACGT]GG
540 CCCGG
BstUI 6 CGCG CG^CG
248 CGCG
CfoI 7 GCGC GCG^C
247 GCGC
DraII 233 GGGACCC [GA]G^G[ACGT]CC[CT]
601 GGGGCCT
EaeI 2 TGGCCG [CT]^GGCC[GA]
250 CGGCCA
EcoO109I 233 GGGACCC [GA]G^G[ACGT]CC[CT]
601 GGGGCCT
HhaI 7 GCGC GCG^C
247 GCGC
HinP1I 7 GCGC G^CGC
247 GCGC
HphI 260 TCACC GGTGA(8/7)
683 TCACC
MaeIII 197 GTGAC ^GT[ACGT]AC
659 GTAAC
MvnI 6 CGCG CG^CG
248 CGCG
MwoI 75 GCCACCCAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
395 GCTTTTGTGGC
NlaIII 408 CATG CATG^
613 CATG
ScrFI 238 CCTGG CC^[ACGT]GG
540 CCCGG
SfaNI 67 GATGC GCATC(5/9)
279 GATGC
TseI 442 GCAGC G^C[AT]GC
676 GCTGC
Tsp509I 375 AATT ^AATT
474 AATT
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# 3 OR MORE CUT ENZYMES ( 24 enzymes) #
#########################################
AciI AluI BsaJI BsiYI BslI
BsrI BstF5I Cac8I Cfr13I DpnII
Fnu4HI FokI HaeIII HinfI ItaI
MboI MboII MnlI NdeII NlaIV
Sau3AI Sau96I TfiI TspRI