Restriction map for
pFN21AE4327
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# NO CUT ENZYMES ( 151 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI AhdI Aor51HI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI AviII AvrII BamHI
BbeI BbrPI BbsI BcgI BciVI
BclI BfrI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BsiEI BsiWI
BsmBI BsmI BsoBI Bsp1407I BspEI
BspHI BspLU11I BsrBI BsrDI BsrGI
BssHII Bst1107I BstBI BstUI BstYI
BstZ17I CelII CfoI CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoRI EcoRV
FbaI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI KasI KpnI KspI
MaeII MamI MfeI MflI MluI
MroI MseI MspA1I MunI MvnI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI TfiI Tru9I
Tth111I XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 32 enzymes) #
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AfaI 518 GTAC GT^AC
Alw44I 407 GTGCAC G^TGCAC
ApaLI 407 GTGCAC G^TGCAC
BanI 178 GGCACC G^G[CT][GA]CC
BbvCI 119 CCTCAGC CCTCAGC(-5/-2)
BglI 975 GCCCACGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BspDI 301 ATCGAT AT^CGAT
BsrFI 984 GCCGGT [GA]^CCGG[CT]
BstAPI 400 GCAGTGAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 684 GGTGACC G^GT[ACGT]ACC
BstPI 684 GGTGACC G^GT[ACGT]ACC
BstXI 362 CCAGGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 984 GCCGGT [GA]^CCGG[CT]
ClaI 301 ATCGAT AT^CGAT
EcoO65I 684 GGTGACC G^GT[ACGT]ACC
EcoT22I 1 ATGCAT ATGCA^T
FauI 395 GCGGG CCCGC(4/6)
HinfI 227 GAGTC G^A[ACGT]TC
NsiI 1 ATGCAT ATGCA^T
NspI 478 GCATGT [GA]CATG^[CT]
PflMI 341 CCAGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 227 GAGTC GAGTC(4/5)
PstI 43 CTGCAG CTGCA^G
RsaI 518 GTAC GT^AC
SfcI 43 CTGCAG C^T[GA][CT]AG
SfiI 974 GGCCCACGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
StuI 644 AGGCCT AGG^CCT
TaqI 302 TCGA T^CGA
Tsp509I 453 AATT ^AATT
TthHB8I 302 TCGA T^CGA
Van91I 341 CCAGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 262 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 22 enzymes) #
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AciI 373 GCGG CCGC(-3/-1)
395 GCGG
AlwNI 34 CAGACACTG CAG[ACGT][ACGT][ACGT]^CTG
342 CAGCCCCTG
AocI 162 CCTGAGG CC^T[ACGT]AGG
426 CCTGAGG
ApaI 115 GGGCCC GGGCC^C
973 GGGCCC
AspHI 407 GTGCAC G[AT]GC[AT]^C
435 GTGCTC
BcnI 512 CCCGG CC^[GC]GG
526 CCCGG
BfaI 106 CTAG C^TAG
263 CTAG
BsgI 92 CTGCAC GTGCAG(16/14)
207 CTGCAC
BsiHKAI 407 GTGCAC G[AT]GC[AT]^C
435 GTGCTC
BsmFI 330 GGGAC GGGAC(10/14)
129 GTCCC
BssSI 534 CACGAG CACGAG(-5/-1)
438 CTCGTG
Bsu36I 162 CCTGAGG CC^T[ACGT]AGG
426 CCTGAGG
DsaI 57 CCACGG C^C[GA][CT]GG
977 CCACGG
EarI 355 CTCTTC CTCTTC(1/4)
640 GAAGAG
Eco81I 162 CCTGAGG CC^T[ACGT]AGG
426 CCTGAGG
Ksp632I 355 CTCTTC CTCTTC(1/4)
640 GAAGAG
MaeI 106 CTAG C^TAG
263 CTAG
MboII 640 GAAGA GAAGA(8/7)
356 TCTTC
MslI 379 CACATAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
410 CACCGTCGTG
NciI 512 CCCGG CC^[GC]GG
526 CCCGG
Tsp45I 235 GTCAC ^GT[GC]AC
685 GTGAC
XcmI 473 CCACTGCATGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
868 CCACCCTGGGGGTGG
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# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AluI AlwI AvaII BalI BanII
BbvI BmyI BpmI BsaJI BseRI
BsiYI BslI BsmAI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII DraII EaeI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HpaII HphI ItaI
MaeIII MboI MluNI MnlI MscI
MspI MvaI MwoI NdeII NlaIII
NlaIV Sau3AI Sau96I ScrFI SfaNI
StyI TseI TspRI